FastQCFastQC Report
Wed 25 May 2016
SRR2088477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230691
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT235761.0568922365311915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT213250.9559818011548888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163900.7347499048501115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87960.3943172765748371No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG62500.28018223949439885No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA54080.24243608818971343No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG51420.2305115320768318No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA51340.23015289881027898No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA46980.21060738578314972No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG41890.18778934419872587No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA30010.13453230411563055No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA25820.11574888677992604No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC25410.11391089128884278No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25210.1130143081224607No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA23380.10481057215006472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65600.023.6326241
TATACCG1351.8189894E-1220.5555555
TTAACGG4750.017.52631635
GTATTAG7650.017.1699351
TAACGGC4950.016.81818236
TCTATCG1655.4569682E-1116.81818229
GTATTGG9850.016.3401011
TAGGGGT3200.015.6093764
ATTAGAG7000.015.5928563
GCACCGC6650.015.30075310
GTATCAA102050.015.1734442
AACGGCC5750.015.12173837
ACACCGT1353.976711E-715.0740756
GTGACAC6550.014.96946624
TAATACT8200.014.89024454
CACATGT6400.014.74218828
AATACTG8000.014.568755
GACACAT7000.014.53571426
GGCACCG6950.014.3741019
TTTAACG6200.014.32258134
Produced by FastQC (version 0.11.5)