FastQCFastQC Report
Wed 25 May 2016
SRR2088476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2858211
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT241000.8431847753717273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211820.7410929424034824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT166180.5814126388849529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92750.3245036842976253No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG57770.20211943764823523No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG48570.16993147111952198No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA46470.16258421789014177No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46430.16244427020958213No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA45300.15849074823377282No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG39910.13963279827836364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71650.023.5219821
CGCGATT500.007037380218.49999827
TAATACT10200.017.5931384
GTATTGG9050.017.580111
TACTGGT9650.016.8704667
CTAATAC10400.016.543273
CTCTAAT8800.016.3977281
TATACCG2050.016.2439045
ATACTGG10100.015.9356446
TCTAATA9900.015.8838392
GTATTAG10000.015.7249991
AATACTG10850.015.3456225
GTATCAA110600.015.3218812
ATGCTAG9400.014.95744715
GGTAAAC9250.014.835
TGCTAGA9950.014.13065316
TCTATAC2650.013.9622643
GCTAGAG10300.013.83009617
ATAGAAC2304.0017767E-1113.6739123
GTGATGC10150.013.669950512
Produced by FastQC (version 0.11.5)