FastQCFastQC Report
Wed 25 May 2016
SRR2088475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319654
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT162411.2307013808164866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142221.0777067322192029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106600.8077874958132966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59660.4520881988763721No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36780.2787094192871768No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG34680.2627961571745321No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32360.24521579141199135No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA31520.23885048656693345No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31270.23695605060114244No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG26960.20429597455090498No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18830.14268891694338062No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16670.1263209901989461No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16220.12291100546052224No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA16090.12192589875831088No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15610.1182885817039921No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA15100.11442393233377841No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14030.10631574640019277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.024.092091
TATCGCG400.001931338623.12516
CTATATC1700.019.5882362
GTATTGG5250.019.028571
CTATCCT701.2193712E-418.54
AATCGAC609.2378026E-418.519
TCGCGCA752.0675584E-417.26666618
TACTGGT3350.016.0149257
AATACTG3050.015.7704915
CTCTAAT2350.015.7446811
GTATCAA70550.015.7072992
GTTTAGG2250.015.6222221
ATACTGG2850.015.5789476
ATTGGAC6200.015.51612953
CTAATAC2750.015.4727273
TATCCTC1201.9359304E-615.4166675
TAATACT3150.015.2698414
TCTATCG1101.4521136E-515.13636429
TCTAATA3450.015.0144932
TACCCCG1853.0559022E-1015.0000015
Produced by FastQC (version 0.11.5)