FastQCFastQC Report
Wed 25 May 2016
SRR2088474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2612090
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331061.2674142162023514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT289091.1067382823715877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT227680.8716391854798266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123280.47195923570780485No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81070.3103644973948065No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA72060.27587104579091837No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG70410.26955426497555596No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA66910.25615503294296904No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61660.23605618489408864No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55400.2120907013158046No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38910.14896117668227357No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36700.14050051874169725No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC35240.13491112480810385No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA34830.13334150048428653No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA34010.13020225183665188No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.12304323357924116No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA29080.11132847643075085No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.10596878361771608No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG27060.10359520537194353No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26860.10282953497008143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91550.024.4713291
TCTAACG2000.017.5752
GTATTGG10600.017.4528311
GTACGAC650.001580662217.0769233
GTATCAA141100.015.8384122
TGTTACG2150.015.48837216
ATTGGAC13200.014.9962123
TAATACT7650.014.99346354
TTGGACC16050.014.9844254
TGGACCC16500.014.9121225
GTATTAG5250.014.8000011
TAGTACT5000.014.7999994
ACCGTTA3900.014.7051298
AATACTG7200.014.3888895
CTAATAC6650.014.1879693
CTCTAAT5750.014.1565221
GGACCCT17550.014.1253576
TCGAGTA1203.3035212E-513.8749992
ATACTGG5900.013.796616
ATTAGAG3900.013.7564113
Produced by FastQC (version 0.11.5)