FastQCFastQC Report
Wed 25 May 2016
SRR2088473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2974337
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331061.1130547748960524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT286000.9615588280682383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225780.7590935391651988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127420.4283979925610313No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG72660.2442897358302035No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG67970.22852151588740616No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63270.2127196750065645No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA63260.21268605406852015No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA56350.18945398587987844No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55810.18763845522548386No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36940.12419574513580674No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA34570.11622758281929721No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA33110.11131892586482299No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.11125168398873428No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32980.11088185367024651No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA29890.10049298381454423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98600.022.9092311
TAATACT8350.017.724554
GTATTGG9950.017.6633171
AATACTG9850.016.7157345
TACTGGT8300.016.4939777
CTCTAAT8050.016.316771
GCGCATA700.002593791815.85714226
CTAATAC9100.015.6538463
ATACTGG9000.015.4166676
ATGCTAG8250.015.24848615
GTATCAA150500.014.9843852
ACCGTTA4850.014.8762888
TGCTAGA8100.014.84567916
TAACACT3250.014.8000014
TCTAATA9300.014.7204312
GACCGTG2151.2732926E-1114.6279077
TTGGACC16000.014.5687494
ATTGGAC12900.014.3410853
GTATTAG8300.014.265061
GATGCTA8300.014.2650614
Produced by FastQC (version 0.11.5)