##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088473_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2974337
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420529684430512	31.0	31.0	34.0	30.0	34.0
2	31.61380435370975	31.0	31.0	34.0	30.0	34.0
3	31.72470369026778	31.0	31.0	34.0	30.0	34.0
4	35.418477126162905	37.0	35.0	37.0	33.0	37.0
5	35.29195447590505	37.0	35.0	37.0	33.0	37.0
6	35.35623468356141	37.0	35.0	37.0	33.0	37.0
7	35.18886494704534	37.0	35.0	37.0	32.0	37.0
8	35.23121757890918	37.0	35.0	37.0	32.0	37.0
9	36.778749011964685	39.0	37.0	39.0	32.0	39.0
10	36.6252274036197	39.0	35.0	39.0	32.0	39.0
11	36.66632664691325	39.0	35.0	39.0	32.0	39.0
12	36.513686915773164	38.0	35.0	39.0	32.0	39.0
13	36.53571199228601	38.0	35.0	39.0	32.0	39.0
14	37.50104880516229	39.0	36.0	41.0	32.0	41.0
15	37.53194980931885	39.0	36.0	41.0	32.0	41.0
16	37.394716536828206	39.0	36.0	41.0	32.0	41.0
17	37.46284466084374	39.0	36.0	41.0	32.0	41.0
18	37.33859142390388	39.0	36.0	41.0	32.0	41.0
19	37.421337931781096	39.0	36.0	41.0	32.0	41.0
20	37.32283228161435	39.0	36.0	41.0	31.0	41.0
21	37.167615841782556	39.0	36.0	40.0	31.0	41.0
22	37.07021531184933	39.0	36.0	40.0	31.0	41.0
23	36.875053163108284	39.0	36.0	40.0	30.0	41.0
24	37.079376008838274	39.0	36.0	41.0	31.0	41.0
25	37.03399715634106	39.0	36.0	41.0	31.0	41.0
26	36.862303767192486	39.0	36.0	40.0	30.0	41.0
27	36.79903689460878	39.0	35.0	40.0	30.0	41.0
28	36.612730837158	39.0	35.0	40.0	30.0	41.0
29	36.44940637190742	39.0	35.0	40.0	30.0	41.0
30	36.278159132606696	38.0	35.0	40.0	30.0	41.0
31	36.01013301451719	38.0	35.0	40.0	29.0	41.0
32	35.861400372587234	38.0	35.0	40.0	29.0	41.0
33	35.66023520535837	38.0	34.0	40.0	27.0	41.0
34	35.544439651592945	38.0	34.0	40.0	27.0	41.0
35	35.304340765689965	38.0	34.0	40.0	26.0	41.0
36	35.15490141164233	38.0	34.0	40.0	25.0	41.0
37	34.99876443052687	38.0	33.0	40.0	25.0	41.0
38	34.913322532046635	38.0	33.0	40.0	24.0	41.0
39	34.81704258797843	38.0	33.0	40.0	24.0	41.0
40	34.70024008711857	38.0	33.0	40.0	24.0	41.0
41	34.48577447679937	38.0	33.0	40.0	23.0	41.0
42	34.2623048430625	38.0	33.0	40.0	22.0	41.0
43	33.91678851454963	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	6.0
14	14.0
15	20.0
16	57.0
17	109.0
18	239.0
19	654.0
20	1381.0
21	2743.0
22	4963.0
23	8640.0
24	13738.0
25	20597.0
26	29385.0
27	41265.0
28	55053.0
29	71824.0
30	89740.0
31	110364.0
32	135594.0
33	164838.0
34	201200.0
35	243809.0
36	307793.0
37	408913.0
38	528572.0
39	532822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3678409675837	21.391086484147557	13.658606943328882	23.582465604939856
2	19.309849556388535	23.519157378602358	34.468017578371246	22.702975486637865
3	19.67846952110672	24.76716659880841	32.48915640695725	23.065207473127625
4	13.956522075339814	16.61923312657577	36.13430488878698	33.28993990929743
5	12.511830367574353	38.76373121135904	35.02471307050949	13.699725350557115
6	32.478464948659145	37.48976662698275	13.588440045630337	16.443328378727763
7	26.224701504906804	32.82180196796799	21.527318525103244	19.426178002021963
8	27.17993959662271	34.46240288171784	18.8336089689904	19.52404855266905
9	24.858481066536843	14.2704743947979	21.361634542420717	39.50940999624454
10	16.532827315801807	27.664148346337353	33.441334993311116	22.361689344549728
11	33.605976726914264	22.559178734622204	23.06500574749936	20.769838790964172
12	21.146561401751047	27.371074629404806	30.629010767777824	20.853353201066323
13	31.195422711010888	21.128843167401676	25.836211565804412	21.83952255578302
14	20.681180377341235	22.945315208061494	27.49671607487652	28.87678833972075
15	23.021231286165623	29.5597976960916	25.301571409023254	22.117399608719523
16	22.190760495532285	28.319353186945527	26.286093337775778	23.20379297974641
17	21.9882615856912	26.921529066813882	26.860338959573177	24.229870387921746
18	22.86331374017134	25.865260056274725	29.15695161644427	22.114474587109665
19	23.009800167230544	25.09063364373304	30.100825831101186	21.79874035793523
20	23.678554245870593	24.872635481453514	29.94701003954831	21.501800233127586
21	22.681996021298193	25.711444264721848	30.24956486100936	21.356994852970594
22	21.63514087341145	26.119232622261702	29.804558125054427	22.441068379272423
23	22.463426303071913	26.19545128880823	30.69174743816857	20.649374969951285
24	22.509419746316574	26.31756253578529	28.25049750583071	22.92252021206743
25	22.360680716408396	26.33269195790524	29.523991397074372	21.78263592861199
26	22.14274979600496	27.023266025336067	29.508290419007665	21.32569375965131
27	21.32824895094268	26.923210113716102	29.028217044672477	22.720323890668745
28	21.586659480751507	26.683795413902324	29.38436364137621	22.345181463969954
29	22.099849479060378	26.553749625546804	28.791290294273985	22.555110601118837
30	21.8494407325061	26.734058716278618	30.324068859715624	21.09243169149965
31	22.30870274619184	26.588446433608564	30.08700762556496	21.015843194634638
32	21.812995635666034	26.3896794478904	29.245778134757426	22.551546781686138
33	21.57637147370994	26.45234887640506	29.832026431436653	22.139253218448346
34	21.097945525338922	26.716441344743387	30.36061481936983	21.824998310547862
35	20.78469924557977	28.529786638165078	29.239894470599666	21.445619645655487
36	22.7755630918756	26.52994600141141	29.03349553194544	21.660995374767552
37	21.648219418310703	27.03274712986457	29.175409511430615	22.14362394039411
38	21.528898709191328	25.83368999545109	30.43864901657075	22.198762278786834
39	20.987063671668675	25.78386376526937	31.049406977084303	22.17966558597765
40	20.229886525972006	26.31561252137871	30.748196993145026	22.706303959504254
41	21.292207305359142	25.138509859508183	30.94340015943049	22.625882675702183
42	20.073448301251673	26.941230936507864	30.503671910748515	22.481648851491943
43	20.500972149423554	26.428478010393576	29.863125799127673	23.207424041055198
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	358.0
2	528.0
3	1255.5
4	1983.0
5	1983.0
6	3167.5
7	4352.0
8	4878.5
9	5405.0
10	8405.0
11	11405.0
12	11405.0
13	21911.0
14	32417.0
15	50504.5
16	68592.0
17	62558.0
18	56524.0
19	56524.0
20	60619.5
21	64715.0
22	52042.0
23	39369.0
24	44217.5
25	49066.0
26	49066.0
27	54649.0
28	60232.0
29	66230.5
30	72229.0
31	81648.5
32	91068.0
33	91068.0
34	102273.0
35	113478.0
36	125054.0
37	136630.0
38	146056.0
39	155482.0
40	155482.0
41	159910.0
42	164338.0
43	175125.0
44	185912.0
45	192734.0
46	199556.0
47	199556.0
48	246717.5
49	293879.0
50	278397.5
51	262916.0
52	243141.5
53	223367.0
54	223367.0
55	201050.0
56	178733.0
57	154440.5
58	130148.0
59	116632.5
60	103117.0
61	103117.0
62	92013.5
63	80910.0
64	70208.5
65	59507.0
66	51339.0
67	43171.0
68	43171.0
69	36995.0
70	30819.0
71	25958.5
72	21098.0
73	17400.5
74	13703.0
75	13703.0
76	11169.5
77	8636.0
78	6781.5
79	4927.0
80	3858.0
81	2789.0
82	2789.0
83	2139.5
84	1490.0
85	1131.5
86	773.0
87	598.0
88	423.0
89	423.0
90	358.5
91	294.0
92	198.5
93	103.0
94	75.5
95	48.0
96	48.0
97	29.0
98	10.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2974337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.313179283808324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27890706995721	38.30216050695222
2	11.727137086615246	11.3315055310288
3	3.8616878938850534	5.597112586661422
4	1.6302099578536948	3.1504250385614068
5	0.8472270641613445	2.04661165224608
6	0.519221500365876	1.505114486111071
7	0.32611133677154475	1.1028832835948224
8	0.23768141281666025	0.9186515767872131
9	0.18082008833745367	0.7862394011365311
>10	1.1554312258888124	10.836527256196726
>50	0.13049134091833453	4.355067658483815
>100	0.09083248039285664	8.711333157844011
>500	0.00879624655162173	2.961128829527649
>1k	0.004747180678638679	3.843355938364146
>5k	4.188688834092953E-4	1.2793760252567223
>10k+	2.7924592227286345E-4	3.272507071247462
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33106	1.1130547748960524	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28600	0.9615588280682383	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22578	0.7590935391651988	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12742	0.4283979925610313	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7266	0.2442897358302035	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6797	0.22852151588740616	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6327	0.2127196750065645	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6326	0.21268605406852015	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5635	0.18945398587987844	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5581	0.18763845522548386	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3694	0.12419574513580674	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3457	0.11622758281929721	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3311	0.11131892586482299	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3309	0.11125168398873428	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3298	0.11088185367024651	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2989	0.10049298381454423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.3620938044344E-5	0.0
3	0.0	0.0	0.0	3.3620938044344E-5	0.0
4	0.0	0.0	0.0	3.3620938044344E-5	0.0
5	3.3620938044344E-5	0.0	0.0	3.3620938044344E-5	0.0
6	6.7241876088688E-5	0.0	0.0	3.3620938044344E-5	0.0
7	6.7241876088688E-5	0.0	0.0	3.3620938044344E-5	0.0
8	1.00862814133032E-4	0.0	0.0	3.3620938044344E-5	0.0
9	2.01725628266064E-4	0.0	0.0	3.3620938044344E-4	0.0
10	2.01725628266064E-4	0.0	0.0	3.69830318487784E-4	0.0
11	2.01725628266064E-4	0.0	0.0	4.37072194576472E-4	0.0
12	2.01725628266064E-4	0.0	0.0	5.71555946753848E-4	0.0
13	2.35346566310408E-4	0.0	0.0	6.387978228425361E-4	0.0
14	4.03451256532128E-4	0.0	0.0	7.73281575019912E-4	0.0
15	4.03451256532128E-4	0.0	0.0	0.001075870017419008	0.0
16	4.03451256532128E-4	0.0	0.0	0.001580184088084168	0.0
17	4.03451256532128E-4	0.0	0.0	0.00235346566310408	0.0
18	4.03451256532128E-4	0.0	0.0	0.002756916919636208	0.0
19	5.0431407066516E-4	0.0	0.0	0.003160368176168336	0.0
20	5.0431407066516E-4	0.0	0.0	0.005345729149050696	0.0
21	5.0431407066516E-4	0.0	0.0	0.008539718263263376	0.0
22	5.0431407066516E-4	0.0	0.0	0.013414754279693256	0.0
23	5.0431407066516E-4	0.0	0.0	0.016944952774349376	0.0
24	5.0431407066516E-4	0.0	0.0	0.022559649427754826	0.0
25	5.0431407066516E-4	0.0	0.0	0.02696399231156389	0.0
26	5.37935008709504E-4	0.0	0.0	0.03627699214984718	0.0
27	5.37935008709504E-4	0.0	0.0	0.09420586840025189	0.0
28	5.37935008709504E-4	0.0	0.0	0.2040118520530794	0.0
29	6.05176884798192E-4	0.0	0.0	0.34273184242404275	0.0
30	6.05176884798192E-4	0.0	0.0	0.5327237633126307	0.0
31	7.06039698931224E-4	0.0	0.0	0.7517305537334875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9860	0.0	22.909231	1
TAATACT	835	0.0	17.72455	4
GTATTGG	995	0.0	17.663317	1
AATACTG	985	0.0	16.715734	5
TACTGGT	830	0.0	16.493977	7
CTCTAAT	805	0.0	16.31677	1
GCGCATA	70	0.0025937918	15.857142	26
CTAATAC	910	0.0	15.653846	3
ATACTGG	900	0.0	15.416667	6
ATGCTAG	825	0.0	15.248486	15
GTATCAA	15050	0.0	14.984385	2
ACCGTTA	485	0.0	14.876288	8
TGCTAGA	810	0.0	14.845679	16
TAACACT	325	0.0	14.800001	4
TCTAATA	930	0.0	14.720431	2
GACCGTG	215	1.2732926E-11	14.627907	7
TTGGACC	1600	0.0	14.568749	4
ATTGGAC	1290	0.0	14.341085	3
GTATTAG	830	0.0	14.26506	1
GATGCTA	830	0.0	14.26506	14
>>END_MODULE