FastQCFastQC Report
Wed 25 May 2016
SRR2088472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404890
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT304581.2665028338094466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT266351.10753506397382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT209980.8731376487074253No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117680.4893363106004849No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG71710.2981841165292383No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG64960.27011630469584885No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA60230.25044804544074784No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA57280.23818137212097018No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA56860.23643493049578151No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG51570.21443808240709555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34900.14512098266448778No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA32030.13318696489236515No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC31450.13077521217186647No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.13077521217186647No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31430.13069204828495273No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31020.12898718860322095No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27700.11518198337553902No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26340.10952683906540425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94200.023.2133751
GTATTGG9900.018.3131311
GTATTAG4450.017.8764041
ACCGTTA4250.016.976478
TAGGTCG904.4492073E-516.44444521
CGTTTAC4550.016.26373728
CTAATAC6700.016.0149253
ATTGGAC12550.015.7729093
TTGGACC15850.015.7570994
TAAACGT1557.219569E-915.51612954
CCGTTTA4650.015.51612927
TATACCG2150.015.4883725
GTATCAA143250.015.2261772
TGTTACG2251.8189894E-1214.816
CGCCGTT4750.014.825
ATACTGG6400.014.7421886
CGAACTA4650.014.72042924
TGGACCC15850.014.7066265
TATACTG2650.014.66037755
GACCGTT5050.014.6534667
Produced by FastQC (version 0.11.5)