##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088472_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2404890
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389751298396185	31.0	31.0	34.0	30.0	34.0
2	31.576014287555772	31.0	31.0	34.0	30.0	34.0
3	31.684049998128813	31.0	31.0	34.0	30.0	34.0
4	35.371028196715855	37.0	35.0	37.0	33.0	37.0
5	35.25415923389427	37.0	35.0	37.0	33.0	37.0
6	35.32185713275867	37.0	35.0	37.0	33.0	37.0
7	35.1461796589449	37.0	35.0	37.0	32.0	37.0
8	35.208427412480404	37.0	35.0	37.0	32.0	37.0
9	36.73447101530631	39.0	35.0	39.0	32.0	39.0
10	36.593855852034814	39.0	35.0	39.0	32.0	39.0
11	36.62632303348594	39.0	35.0	39.0	32.0	39.0
12	36.471626976701636	38.0	35.0	39.0	32.0	39.0
13	36.49621521150655	38.0	35.0	39.0	32.0	39.0
14	37.44074365147678	39.0	36.0	41.0	32.0	41.0
15	37.4712223843918	39.0	36.0	41.0	32.0	41.0
16	37.3239408039453	39.0	36.0	41.0	32.0	41.0
17	37.415293838803436	39.0	36.0	41.0	32.0	41.0
18	37.29543347096956	39.0	36.0	41.0	32.0	41.0
19	37.379806976618475	39.0	36.0	41.0	32.0	41.0
20	37.28541929152685	39.0	36.0	41.0	31.0	41.0
21	37.13825372470259	39.0	36.0	40.0	31.0	41.0
22	37.04040392699874	39.0	36.0	40.0	31.0	41.0
23	36.842777008511824	39.0	35.0	40.0	30.0	41.0
24	37.03742416493062	39.0	36.0	40.0	31.0	41.0
25	36.99193435042767	39.0	36.0	41.0	31.0	41.0
26	36.80951062210745	39.0	36.0	40.0	30.0	41.0
27	36.74378412318235	39.0	35.0	40.0	30.0	41.0
28	36.552804909995885	39.0	35.0	40.0	30.0	41.0
29	36.378883441654295	39.0	35.0	40.0	30.0	41.0
30	36.20575660425217	38.0	35.0	40.0	30.0	41.0
31	35.919374690734294	38.0	35.0	40.0	29.0	41.0
32	35.77135918898578	38.0	34.0	40.0	28.0	41.0
33	35.55840183958518	38.0	34.0	40.0	27.0	41.0
34	35.44784792651639	38.0	34.0	40.0	27.0	41.0
35	35.20174103597254	38.0	34.0	40.0	26.0	41.0
36	35.0361380354195	38.0	34.0	40.0	25.0	41.0
37	34.87054917272724	38.0	33.0	40.0	25.0	41.0
38	34.76816153753394	38.0	33.0	40.0	24.0	41.0
39	34.66330435071875	38.0	33.0	40.0	24.0	41.0
40	34.518568001031234	38.0	33.0	40.0	23.0	41.0
41	34.29444881054851	38.0	33.0	40.0	22.0	41.0
42	34.05062975853365	38.0	33.0	40.0	21.0	41.0
43	33.70950646391311	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	5.0
14	7.0
15	18.0
16	41.0
17	87.0
18	220.0
19	556.0
20	1166.0
21	2257.0
22	4095.0
23	7240.0
24	11530.0
25	17399.0
26	24946.0
27	34694.0
28	46363.0
29	59891.0
30	74769.0
31	91937.0
32	111807.0
33	136122.0
34	163908.0
35	198087.0
36	249821.0
37	332459.0
38	418748.0
39	416711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54506027302704	21.42551218558853	13.915272632012273	23.114154909372154
2	19.336476928258673	23.80537155545576	34.49721193069122	22.360939585594352
3	19.991933102969366	24.686243445646163	32.72482317278545	22.597000278599022
4	14.054780052310084	16.43630269991559	36.0643106337504	33.444606614023925
5	12.37611699495611	38.978206903434256	34.800302716548366	13.845373385061272
6	32.35062726361705	37.26814947877034	13.381152568308737	17.000070689303875
7	25.944180399103494	33.16579968314559	21.240805192753097	19.64921472499782
8	27.430194312421776	34.17865266186811	18.6799396230181	19.711213402692014
9	24.65796772409549	14.281401644150044	21.63512676255463	39.425503869199844
10	16.678392774721505	27.618394188507583	33.1060048484546	22.597208188316305
11	33.18954297285946	22.534211543979143	23.330256269517523	20.945989213643866
12	21.229952305510853	27.446161778709214	30.598488912174776	20.725397003605153
13	31.608015335420745	21.016512189746724	26.120862076851747	21.25461039798078
14	20.557322788152472	23.227964688613618	27.5631317856534	28.651580737580513
15	22.78781981712261	29.597902606772035	25.595183147669953	22.019094428435395
16	21.793429221294943	28.413191455742258	26.570653959224742	23.22272536373805
17	21.78977001027074	26.82671556703217	26.903558998540472	24.479955424156614
18	22.945748038371818	25.546906511316525	29.49153599540935	22.015809454902303
19	22.86566121527388	24.806415262236527	30.543808656528988	21.784114865960603
20	23.759298762105544	24.60765357251267	30.365713192703204	21.267334472678584
21	22.60082581739705	25.469397768712916	30.8663182099805	21.063458203909533
22	21.27606668080453	25.887296300454494	30.471331329083657	22.365305689657323
23	22.242139973138066	26.077907929260796	31.419815459334938	20.2601366382662
24	22.305552436909796	26.05383198399927	28.896623130371868	22.74399244871907
25	22.07784971453996	26.357879154555924	29.99035298911801	21.573918141786113
26	21.851436032417283	26.945265687827717	30.056676188931718	21.146622090823282
27	20.93131078760359	27.01620448336514	29.581935140484596	22.47054958854667
28	21.279601145998363	26.593981429504055	29.790718078581556	22.33569934591603
29	21.73313540328248	26.552981633255573	29.26578762438199	22.448095339079956
30	21.612298275596807	26.614065508193725	30.96869295477132	20.80494326143815
31	22.17319711088657	26.547076997284698	30.672837427075667	20.606888464753066
32	21.766733613595633	26.353221976888758	29.58750712090782	22.292537288607793
33	21.40343217361293	26.31534082639955	30.258473360527926	22.0227536394596
34	20.665851660574912	26.88189480599944	30.862035269804437	21.590218263621207
35	20.331241761577452	28.746886551983668	29.711961877674238	21.209909808764642
36	22.544939685391014	26.428069475111126	29.642270540440517	21.384720299057335
37	21.456989716785383	27.05080066032126	29.638486583585944	21.85372303930741
38	21.23814394837186	25.679802402604697	31.100507715529606	21.98154593349384
39	20.718619146821684	25.563664034529648	31.73034941307087	21.9873674055778
40	19.757036704381488	26.23092948118209	31.33977021818046	22.672263596255963
41	21.10038296969924	25.074993035024473	31.40438855831244	22.420235436963853
42	19.75699512243803	27.070053100141795	30.851722947827138	22.321228829593036
43	20.16341703778551	26.691698996627704	30.122001422102468	23.022882543484318
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	288.5
2	439.0
3	1070.5
4	1702.0
5	1702.0
6	2778.0
7	3854.0
8	4434.5
9	5015.0
10	7929.0
11	10843.0
12	10843.0
13	20579.5
14	30316.0
15	46743.0
16	63170.0
17	57323.0
18	51476.0
19	51476.0
20	55224.5
21	58973.0
22	46573.0
23	34173.0
24	37836.0
25	41499.0
26	41499.0
27	45017.0
28	48535.0
29	52283.0
30	56031.0
31	61599.0
32	67167.0
33	67167.0
34	75255.5
35	83344.0
36	92762.0
37	102180.0
38	109770.5
39	117361.0
40	117361.0
41	121089.5
42	124818.0
43	134173.0
44	143528.0
45	152080.5
46	160633.0
47	160633.0
48	207238.5
49	253844.0
50	238616.0
51	223388.0
52	204927.0
53	186466.0
54	186466.0
55	166518.0
56	146570.0
57	125893.0
58	105216.0
59	93666.5
60	82117.0
61	82117.0
62	72621.5
63	63126.0
64	54523.0
65	45920.0
66	39093.0
67	32266.0
68	32266.0
69	27369.0
70	22472.0
71	18889.0
72	15306.0
73	12624.0
74	9942.0
75	9942.0
76	7899.5
77	5857.0
78	4627.5
79	3398.0
80	2604.0
81	1810.0
82	1810.0
83	1386.0
84	962.0
85	743.0
86	524.0
87	392.5
88	261.0
89	261.0
90	203.0
91	145.0
92	101.0
93	57.0
94	44.0
95	31.0
96	31.0
97	22.5
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2404890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.311225203533446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72789965186828	39.80791639941527
2	11.126450623109301	10.973178247842757
3	3.400069726677884	5.029848119997886
4	1.454505490053856	2.8689379111928592
5	0.7567768214135564	1.8658796134769045
6	0.4744480696593063	1.403736936621107
7	0.3143785845448505	1.0851675227161441
8	0.22213716527440422	0.876308462633654
9	0.17081496031004076	0.7580785478382942
>10	1.1196231594717097	10.726036042747339
>50	0.12835934062155094	4.347432253322914
>100	0.09209761875442324	8.838539956830509
>500	0.0070232613876747355	2.3369051680716417
>1k	0.004569350782342573	3.81955437239062
>5k	5.077056424825081E-4	1.513025716877574
>10k+	3.384704283216721E-4	3.749454728024653
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30458	1.2665028338094466	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26635	1.10753506397382	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20998	0.8731376487074253	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11768	0.4893363106004849	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7171	0.2981841165292383	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6496	0.27011630469584885	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6023	0.25044804544074784	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5728	0.23818137212097018	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5686	0.23643493049578151	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5157	0.21443808240709555	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3490	0.14512098266448778	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3203	0.13318696489236515	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3145	0.13077521217186647	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3145	0.13077521217186647	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3143	0.13069204828495273	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3102	0.12898718860322095	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2770	0.11518198337553902	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2634	0.10952683906540425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.158194345687329E-5	0.0	0.0	0.0	0.0
6	4.158194345687329E-5	0.0	0.0	0.0	0.0
7	4.158194345687329E-5	0.0	0.0	0.0	0.0
8	4.158194345687329E-5	0.0	0.0	0.0	0.0
9	4.158194345687329E-5	0.0	0.0	3.326555476549863E-4	0.0
10	4.158194345687329E-5	0.0	0.0	3.326555476549863E-4	0.0
11	1.2474583037061987E-4	0.0	0.0	4.989833214824795E-4	0.0
12	1.2474583037061987E-4	0.0	0.0	7.900569256805924E-4	0.0
13	1.2474583037061987E-4	0.0	0.0	9.148027560512123E-4	0.0
14	1.2474583037061987E-4	0.0	0.0	0.001164294416792452	0.0
15	1.2474583037061987E-4	0.0	0.0	0.0014137860775336917	0.0
16	1.2474583037061987E-4	0.0	0.0	0.001995933285929918	0.0
17	1.6632777382749315E-4	0.0	0.0	0.002869154098524257	0.0
18	1.6632777382749315E-4	0.0	0.0	0.0031186457592654966	0.0
19	2.0790971728436644E-4	0.0	0.0	0.0043661040629716956	0.0
20	2.4949166074123973E-4	0.0	0.0	0.007651077596064685	0.0
21	2.91073604198113E-4	0.0	0.0	0.012183509432863874	0.0
22	2.91073604198113E-4	0.0	0.0	0.019959332859299177	0.0
23	2.91073604198113E-4	0.0	4.158194345687329E-5	0.024616510526468988	0.0
24	3.326555476549863E-4	0.0	4.158194345687329E-5	0.031810186744508064	0.0
25	3.326555476549863E-4	0.0	4.158194345687329E-5	0.036758438015875985	0.0
26	3.326555476549863E-4	0.0	4.158194345687329E-5	0.04628070306749997	0.0
27	3.326555476549863E-4	0.0	4.158194345687329E-5	0.112229665390101	0.0
28	3.742374911118596E-4	0.0	4.158194345687329E-5	0.23672600409997963	0.0
29	3.742374911118596E-4	0.0	4.158194345687329E-5	0.3927414559501682	0.0
30	3.742374911118596E-4	0.0	4.158194345687329E-5	0.6150801076140696	0.0
31	3.742374911118596E-4	0.0	4.158194345687329E-5	0.8658608086024725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9420	0.0	23.213375	1
GTATTGG	990	0.0	18.313131	1
GTATTAG	445	0.0	17.876404	1
ACCGTTA	425	0.0	16.97647	8
TAGGTCG	90	4.4492073E-5	16.444445	21
CGTTTAC	455	0.0	16.263737	28
CTAATAC	670	0.0	16.014925	3
ATTGGAC	1255	0.0	15.772909	3
TTGGACC	1585	0.0	15.757099	4
TAAACGT	155	7.219569E-9	15.5161295	4
CCGTTTA	465	0.0	15.516129	27
TATACCG	215	0.0	15.488372	5
GTATCAA	14325	0.0	15.226177	2
TGTTACG	225	1.8189894E-12	14.8	16
CGCCGTT	475	0.0	14.8	25
ATACTGG	640	0.0	14.742188	6
CGAACTA	465	0.0	14.720429	24
TGGACCC	1585	0.0	14.706626	5
TATACTG	265	0.0	14.6603775	5
GACCGTT	505	0.0	14.653466	7
>>END_MODULE