FastQCFastQC Report
Wed 25 May 2016
SRR2088471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614461
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141850.8786214098699195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT123990.7679962538580988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95800.5933868950690044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54370.3367687420135884No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30790.19071380479305478No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29710.18402426568371735No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26580.1646369903020265No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA26380.1633981867632603No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25130.15565566464597164No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23140.14332956943524805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTT451.3235361E-424.6666687
GGTATCA41150.023.7375451
TTATGCG2000.020.354
TATGCGG2050.019.8536575
TCGGGTA1457.2759576E-1219.1379325
TAATACT5950.018.0336154
GTATTAG3650.017.7397251
ATACTGG5150.017.601946
CTCTAAT4900.017.3673461
CGGGTAA1502.5102054E-1017.26666626
CTAATAC5800.016.9051723
ACCGTTA3200.016.7656258
GTCGGGT1901.8189894E-1216.5526324
TCTAATA5850.016.1282062
AATACTG6350.015.7322845
TAGCGAA2850.015.57894810
CGAATTA957.062507E-515.57894615
TAGAGGT5150.015.44660119
GTATCAA63250.015.3849812
GTATTGG4500.015.2111111
Produced by FastQC (version 0.11.5)