##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088471_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1614461
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39069447945785	31.0	31.0	34.0	30.0	34.0
2	31.589944260034773	31.0	31.0	34.0	30.0	34.0
3	31.70233161408049	31.0	31.0	34.0	30.0	34.0
4	35.40760352836024	37.0	35.0	37.0	33.0	37.0
5	35.275205780752835	37.0	35.0	37.0	33.0	37.0
6	35.328755541323076	37.0	35.0	37.0	33.0	37.0
7	35.17182019262156	37.0	35.0	37.0	32.0	37.0
8	35.20028665913887	37.0	35.0	37.0	32.0	37.0
9	36.757826296206595	39.0	35.0	39.0	32.0	39.0
10	36.591378794532666	39.0	35.0	39.0	32.0	39.0
11	36.631603984239945	39.0	35.0	39.0	32.0	39.0
12	36.47082772516648	38.0	35.0	39.0	32.0	39.0
13	36.492817726783116	38.0	35.0	39.0	32.0	39.0
14	37.476917683363055	39.0	36.0	41.0	32.0	41.0
15	37.49324635280753	39.0	36.0	41.0	32.0	41.0
16	37.358910497063725	39.0	36.0	41.0	32.0	41.0
17	37.40406984126591	39.0	36.0	41.0	32.0	41.0
18	37.270884834009614	39.0	36.0	40.0	32.0	41.0
19	37.33975921375617	39.0	36.0	41.0	31.0	41.0
20	37.235078456525116	39.0	36.0	40.0	31.0	41.0
21	37.0810381916937	39.0	36.0	40.0	31.0	41.0
22	36.97748040987054	39.0	36.0	40.0	31.0	41.0
23	36.78563557744659	39.0	35.0	40.0	30.0	41.0
24	37.007915335210946	39.0	36.0	40.0	31.0	41.0
25	36.96278014767777	39.0	36.0	40.0	31.0	41.0
26	36.800462817002085	39.0	36.0	40.0	30.0	41.0
27	36.73828355098079	39.0	35.0	40.0	30.0	41.0
28	36.56123127161325	39.0	35.0	40.0	30.0	41.0
29	36.40549136832664	39.0	35.0	40.0	30.0	41.0
30	36.25615360172838	38.0	35.0	40.0	30.0	41.0
31	36.000605774930456	38.0	35.0	40.0	29.0	41.0
32	35.862030114075225	38.0	35.0	40.0	28.0	41.0
33	35.67698693248087	38.0	34.0	40.0	27.0	41.0
34	35.568324660676225	38.0	34.0	40.0	27.0	41.0
35	35.34471876372362	38.0	34.0	40.0	26.0	41.0
36	35.2119976883926	38.0	34.0	40.0	25.0	41.0
37	35.06683592852352	38.0	34.0	40.0	25.0	41.0
38	35.011260724167386	38.0	33.0	40.0	25.0	41.0
39	34.93913696273865	38.0	33.0	40.0	24.0	41.0
40	34.828179187976666	38.0	33.0	40.0	24.0	41.0
41	34.64700417043211	38.0	33.0	40.0	23.0	41.0
42	34.45757376610522	38.0	33.0	40.0	23.0	41.0
43	34.13150952547011	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	3.0
13	4.0
14	3.0
15	5.0
16	30.0
17	56.0
18	132.0
19	328.0
20	772.0
21	1453.0
22	2734.0
23	4599.0
24	7388.0
25	11040.0
26	16097.0
27	22189.0
28	29891.0
29	38577.0
30	48797.0
31	60409.0
32	73762.0
33	90433.0
34	109500.0
35	133038.0
36	168116.0
37	219497.0
38	291939.0
39	283667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.99931803865191	21.5466338301142	13.377529714251382	24.07651841698251
2	19.44141109633494	23.34624373087984	34.175616506066106	23.036728666719107
3	19.497528896641043	24.810447573524538	32.105451912433935	23.58657161740048
4	14.104335750445504	16.788203617182454	36.407259140976464	32.70020149139558
5	12.66936767131569	38.638096553586614	35.19007272396174	13.502463051135951
6	32.54479358745736	37.883603258301065	13.893057806908931	15.67854534733264
7	26.625480578347823	32.5718614447794	21.63198739393519	19.17067058293759
8	27.004802221918027	34.64735289362827	19.013961935283664	19.333882949170032
9	25.126652176794607	14.276281681626251	21.00502892296562	39.59203721861352
10	16.439666241550587	27.565608583917484	33.72264799211626	22.27207718241568
11	33.994007907282985	22.60066982107341	23.00959886922013	20.39572340242347
12	20.99598565713263	27.05268197869134	30.639389864481085	21.311942499694943
13	30.78259555356246	21.22640311534314	25.703067463382517	22.28793386771189
14	20.817288246665605	22.689553974979884	27.066122997086957	29.427034781267558
15	23.23797230159168	29.60158219987971	24.693628399818888	22.466817098709726
16	22.62934812299585	28.223846844240896	25.87427011244	23.272534920323253
17	22.26941375480733	27.216885387754797	26.546506852751477	23.967194004686394
18	22.891107310737144	26.266041731574813	28.485915732866886	22.356935224821164
19	23.20966564073087	25.523874531499985	29.28085596369315	21.985603864075998
20	23.70363855181389	25.207236346991348	29.204483725528213	21.884641375666554
21	22.847996947588083	25.97492289996476	29.406408702347097	21.770671450100064
22	22.131968502181223	26.28617228907976	28.947989452826672	22.633869755912343
23	22.719656900971906	26.36539377538386	29.635401536488033	21.279547787156208
24	22.817522380534434	26.5397553734652	27.63089353041046	23.011828715589907
25	22.692589043649864	26.31577969365627	28.836435194160774	22.15519606853309
26	22.431449257677947	27.028463369508458	28.658481065817014	21.881606306996577
27	21.87318244293297	27.0351529086178	28.215422980177284	22.87624166827195
28	21.992603104070028	26.746201983200585	28.6450400474214	22.616154865307987
29	22.58636164020066	26.689836422186726	28.173675300920863	22.55012663669175
30	22.401098570978178	26.79216159448881	29.293367879434683	21.513371955098325
31	22.61677426707737	26.64443427249094	29.268405988128542	21.470385472303143
32	22.173344540376014	26.569238897687836	28.56643796288669	22.690978599049465
33	22.111280483083828	26.537773287803173	29.01438932250454	22.33655690660846
34	21.68476042468663	26.63774473338161	29.490399582275444	22.18709525965632
35	21.41036544084992	28.053015836245038	28.68226609376132	21.85435262914372
36	23.02465033221614	26.441951834079607	28.41375542673375	22.1196424069705
37	21.946457672250986	26.72619530604951	28.671550443150995	22.655796578548507
38	21.94305096251938	25.78910236914983	29.747513256746373	22.520333411584424
39	21.459607881515876	25.832832134068273	30.3105494651156	22.39701051930025
40	20.825712110729217	26.17108744032838	30.13160429394083	22.871596155001576
41	21.52284880216989	25.16338270171903	30.331733005628504	22.982035490482584
42	20.672967634399345	26.616870893753397	29.82140788783377	22.88875358401349
43	21.00824981216641	26.06120556643982	29.49969060881619	23.430854012577573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	191.0
2	267.0
3	572.0
4	877.0
5	877.0
6	1392.5
7	1908.0
8	2213.5
9	2519.0
10	3840.5
11	5162.0
12	5162.0
13	9691.5
14	14221.0
15	22037.5
16	29854.0
17	27505.0
18	25156.0
19	25156.0
20	27452.0
21	29748.0
22	24788.0
23	19828.0
24	22686.0
25	25544.0
26	25544.0
27	28739.5
28	31935.0
29	35658.5
30	39382.0
31	45046.5
32	50711.0
33	50711.0
34	57722.5
35	64734.0
36	71304.0
37	77874.0
38	83762.0
39	89650.0
40	89650.0
41	92711.5
42	95773.0
43	101666.5
44	107560.0
45	109535.0
46	111510.0
47	111510.0
48	131480.5
49	151451.0
50	144506.5
51	137562.0
52	128225.0
53	118888.0
54	118888.0
55	107976.5
56	97065.0
57	85418.0
58	73771.0
59	66570.5
60	59370.0
61	59370.0
62	52754.0
63	46138.0
64	40078.5
65	34019.0
66	29246.0
67	24473.0
68	24473.0
69	20864.5
70	17256.0
71	14582.5
72	11909.0
73	9734.0
74	7559.0
75	7559.0
76	6190.5
77	4822.0
78	3765.5
79	2709.0
80	2108.0
81	1507.0
82	1507.0
83	1161.5
84	816.0
85	611.5
86	407.0
87	308.5
88	210.0
89	210.0
90	166.0
91	122.0
92	84.0
93	46.0
94	33.0
95	20.0
96	20.0
97	13.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1614461.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.566042490932155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.69979636605026	47.34566237724369
2	9.617226248795015	10.88016857268494
3	2.707578092421339	4.594709322702675
4	1.16788264670305	2.642499976713082
5	0.6288156698230056	1.778480694908605
6	0.3987162951637345	1.3532281736435312
7	0.2675082873552235	1.0592319604448441
8	0.1982236574487822	0.8970182263964637
9	0.15182235307109024	0.7729190707403335
>10	0.9908793874947339	10.815505963868143
>50	0.09720365424771553	3.82185606373198
>100	0.06554900547824559	7.14027946518873
>500	0.006488772761483308	2.4063168629196325
>1k	0.00186964638890197	1.9040884235620785
>5k	2.1995839869434945E-4	0.934220794479424
>10k+	2.1995839869434945E-4	1.6538140507718588
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14185	0.8786214098699195	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12399	0.7679962538580988	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9580	0.5933868950690044	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5437	0.3367687420135884	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3079	0.19071380479305478	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2971	0.18402426568371735	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2658	0.1646369903020265	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2638	0.1633981867632603	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2513	0.15565566464597164	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2314	0.14332956943524805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2388035387661889E-4	0.0
10	0.0	0.0	0.0	1.8582053081492831E-4	0.0
11	0.0	0.0	0.0	3.7164106162985663E-4	0.0
12	0.0	0.0	0.0	5.574615924447849E-4	0.0
13	0.0	0.0	0.0	6.194017693830945E-4	0.0
14	6.194017693830944E-5	0.0	0.0	6.813419463214039E-4	0.0
15	6.194017693830944E-5	0.0	0.0	7.432821232597133E-4	0.0
16	6.194017693830944E-5	0.0	0.0	0.001238803538766189	0.0
17	1.2388035387661889E-4	0.0	0.0	0.0017962651312109737	0.0
18	1.2388035387661889E-4	0.0	0.0	0.0020440258389642116	0.0
19	1.2388035387661889E-4	0.0	0.0	0.0027253677852856154	0.0
20	1.2388035387661889E-4	0.0	0.0	0.00439775256261997	0.0
21	1.2388035387661889E-4	0.0	0.0	0.0065037185785224915	0.0
22	1.2388035387661889E-4	0.0	0.0	0.011582813087463865	0.0
23	1.2388035387661889E-4	0.0	0.0	0.014184300518872862	0.0
24	1.2388035387661889E-4	0.0	0.0	0.018520112904554523	0.0
25	1.2388035387661889E-4	0.0	0.0	0.022422344051668017	0.0
26	1.2388035387661889E-4	0.0	0.0	0.030288746522833317	0.0
27	1.8582053081492831E-4	0.0	0.0	0.07377075073352654	0.0
28	1.8582053081492831E-4	0.0	0.0	0.16296460552469214	0.0
29	1.8582053081492831E-4	0.0	0.0	0.2771822917989348	0.0
30	1.8582053081492831E-4	0.0	0.0	0.4317230332600168	0.0
31	1.8582053081492831E-4	0.0	0.0	0.6218174362836885	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTT	45	1.3235361E-4	24.666668	7
GGTATCA	4115	0.0	23.737545	1
TTATGCG	200	0.0	20.35	4
TATGCGG	205	0.0	19.853657	5
TCGGGTA	145	7.2759576E-12	19.13793	25
TAATACT	595	0.0	18.033615	4
GTATTAG	365	0.0	17.739725	1
ATACTGG	515	0.0	17.60194	6
CTCTAAT	490	0.0	17.367346	1
CGGGTAA	150	2.5102054E-10	17.266666	26
CTAATAC	580	0.0	16.905172	3
ACCGTTA	320	0.0	16.765625	8
GTCGGGT	190	1.8189894E-12	16.55263	24
TCTAATA	585	0.0	16.128206	2
AATACTG	635	0.0	15.732284	5
TAGCGAA	285	0.0	15.578948	10
CGAATTA	95	7.062507E-5	15.578946	15
TAGAGGT	515	0.0	15.446601	19
GTATCAA	6325	0.0	15.384981	2
GTATTGG	450	0.0	15.211111	1
>>END_MODULE