FastQCFastQC Report
Wed 25 May 2016
SRR2088470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579102
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58891.0169192991908163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49910.8618516254476759No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40920.706611270553374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.3785170833462844No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG12770.22051383003339653No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG11990.20704470024278968No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA11790.2035910772195572No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11590.20013745419632467No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA11000.18994926627778871No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG9640.16646462971980758No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6400.1105159367434407No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA6390.11034325559227907No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA6360.1098252121387942No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC6170.10654427026672329No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.10429941530162216No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA5820.10050042997606638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG358.864059E-426.4285725
GTCCGTG400.001930036723.1257
GGTATCA16350.021.2721711
CTAGTGA450.003823549520.55555729
GACCTAG450.003823549520.5555576
GTAAGAC555.1388604E-420.1818183
ACGGGCG701.2178719E-418.55
CGTGTTT500.007030770618.518
GAATTAG609.229272E-418.51
TAGTACC500.007030770618.54
TTATACC852.71934E-517.4117644
TTACACT752.0650354E-417.2666664
ACTGAGC650.001578654517.0769238
GGACCGT1201.0385338E-716.9583346
CGGGTAA803.3789416E-416.187526
CGTTATT803.3789416E-416.187510
TTGGACC2750.016.1454544
TGGACCC2550.015.9607845
ATTATAC700.002590410915.8571433
TACACTT700.002590410915.8571435
Produced by FastQC (version 0.11.5)