##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	579102
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.450347261794985	31.0	31.0	34.0	30.0	34.0
2	31.583738270632807	31.0	31.0	34.0	30.0	34.0
3	31.741442785554185	31.0	31.0	34.0	30.0	34.0
4	35.448520295215694	37.0	35.0	37.0	33.0	37.0
5	35.30482885571109	37.0	35.0	37.0	33.0	37.0
6	35.38245075996975	37.0	35.0	37.0	33.0	37.0
7	35.22371706538745	37.0	35.0	37.0	32.0	37.0
8	35.256913289886754	37.0	35.0	37.0	32.0	37.0
9	36.82119212159516	39.0	37.0	39.0	32.0	39.0
10	36.6658101681569	39.0	35.0	39.0	32.0	39.0
11	36.69852115862145	39.0	35.0	39.0	32.0	39.0
12	36.5584059457574	38.0	35.0	39.0	32.0	39.0
13	36.576815483282736	38.0	35.0	39.0	32.0	39.0
14	37.55167483448512	39.0	36.0	41.0	32.0	41.0
15	37.579276189686794	39.0	36.0	41.0	32.0	41.0
16	37.44271302810213	39.0	36.0	41.0	32.0	41.0
17	37.49243483876761	39.0	36.0	41.0	32.0	41.0
18	37.373656108941084	39.0	36.0	41.0	32.0	41.0
19	37.449950095147315	39.0	36.0	41.0	32.0	41.0
20	37.35034588034578	39.0	36.0	41.0	31.0	41.0
21	37.19541807833508	39.0	36.0	40.0	31.0	41.0
22	37.08537010751129	39.0	36.0	40.0	31.0	41.0
23	36.882319522294864	39.0	36.0	40.0	30.0	41.0
24	37.10851628901299	39.0	36.0	41.0	31.0	41.0
25	37.07031576475301	39.0	36.0	41.0	31.0	41.0
26	36.89687308971477	39.0	36.0	40.0	31.0	41.0
27	36.827622767664415	39.0	35.0	40.0	30.0	41.0
28	36.634836350073044	39.0	35.0	40.0	30.0	41.0
29	36.47695397356597	39.0	35.0	40.0	30.0	41.0
30	36.30401034705458	38.0	35.0	40.0	30.0	41.0
31	36.0631926672676	38.0	35.0	40.0	29.0	41.0
32	35.92580754340341	38.0	35.0	40.0	29.0	41.0
33	35.739306719714314	38.0	34.0	40.0	28.0	41.0
34	35.62077665074546	38.0	34.0	40.0	27.0	41.0
35	35.39303438772444	38.0	34.0	40.0	26.0	41.0
36	35.23845540163909	38.0	34.0	40.0	26.0	41.0
37	35.084038735835826	38.0	34.0	40.0	25.0	41.0
38	35.00420133240776	38.0	33.0	40.0	25.0	41.0
39	34.914735227990924	38.0	33.0	40.0	24.0	41.0
40	34.78846904345003	38.0	33.0	40.0	24.0	41.0
41	34.58977693048893	38.0	33.0	40.0	23.0	41.0
42	34.370566843146804	38.0	33.0	40.0	22.0	41.0
43	34.04201159726611	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	8.0
17	21.0
18	34.0
19	120.0
20	236.0
21	485.0
22	1004.0
23	1573.0
24	2535.0
25	3837.0
26	5501.0
27	7623.0
28	10384.0
29	13354.0
30	16960.0
31	21229.0
32	26380.0
33	32127.0
34	39296.0
35	47654.0
36	60278.0
37	80337.0
38	103851.0
39	104267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41222099043001	21.323013907739917	13.372601027107486	23.892164074722587
2	19.099571405382818	23.52953365728317	34.636903343452445	22.733991593881562
3	19.49397515463597	24.913055040390123	32.34335229372373	23.249617511250175
4	13.958853535301207	16.446498198935593	36.4541997782774	33.1404484874858
5	12.581375992484917	38.532935475961054	35.29619997858754	13.58948855296649
6	32.53261083539687	37.69854015354808	13.791698180976752	15.977150830078294
7	26.31056359674116	32.732230246139714	21.692378890074632	19.264827267044492
8	27.019247041108475	34.39808531139592	19.195754806579842	19.386912840915763
9	24.80322982825133	14.462909815541995	21.240472317484656	39.493388038722024
10	16.332183276866598	27.505171800477292	33.85327627948099	22.309368643175123
11	33.47234166001844	22.6343545696613	23.159823312646132	20.733480457674123
12	20.970571678219034	27.16015486045636	30.906299753756677	20.962973707567926
13	31.047898297709214	21.176407610403693	25.85157709695356	21.924116994933534
14	20.68547509765119	22.998193755158848	27.280340941664853	29.03599020552511
15	23.022023754019152	29.694423434904387	25.063287641900732	22.220265169175725
16	22.241850313070927	28.499815231168256	26.074163100800895	23.184171354959922
17	21.86816830195717	27.118538703026413	26.835514296272507	24.177778698743918
18	22.815324416078685	25.98661375716195	29.002144699897425	22.195917126861932
19	22.88785049956657	25.426436102793637	29.752962345148177	21.932751052491618
20	23.65041046309631	25.1719040859814	29.61309061270726	21.56459483821503
21	22.734682318486207	25.794246954767896	29.96225190035607	21.508818826389824
22	21.780964320620548	26.189686790928025	29.477017865591897	22.55233102285953
23	22.48204979433675	26.412963519380007	30.083301387320365	21.02168529896288
24	22.399853566383815	26.46304105321688	28.233022852623545	22.904082527775763
25	22.38465762508159	26.50914692057703	29.20625382057047	21.899941633770908
26	22.15861799821102	27.117675297270598	29.114042085850162	21.609664618668216
27	21.434047887936842	27.25754702971152	28.742950292003826	22.565454790347815
28	21.80911134825989	26.883865018597756	28.954312021025657	22.35271161211669
29	22.162416983536577	26.92427240796958	28.465279000935933	22.44803160755791
30	21.952782066026366	26.975040666411097	29.889898498019345	21.18227876954319
31	22.514341169603973	26.83499625281902	29.634848437753625	21.015814139823384
32	22.00873075900273	26.65765271057603	28.847940431910096	22.485676098511142
33	21.72035323656282	26.718436475784923	29.406391274766797	22.15481901288547
34	21.087649498706618	27.038241967736255	29.89007117917051	21.98403735438662
35	20.913586898335698	28.601006385748974	28.978487382188284	21.506919333727044
36	22.684777465800497	26.834305528214376	28.728445075306254	21.75247193067888
37	21.778546784504286	27.36357325652476	28.892837531212116	21.965042427758842
38	21.63815700860988	26.152733024579433	30.039267693774153	22.16984227303653
39	21.184005581054805	26.09471215778913	30.663164692921107	22.058117568234955
40	20.39019032916481	26.482208660995816	30.467344267503826	22.660256742335548
41	21.401929193820777	25.540751024862633	30.421238400143668	22.63608138117292
42	20.364115475339407	27.03547906931767	30.107476748483	22.49292870685993
43	20.646794519790987	26.639175827401733	29.50136590790569	23.212663744901587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	52.5
2	86.0
3	200.0
4	314.0
5	314.0
6	518.0
7	722.0
8	852.0
9	982.0
10	1519.5
11	2057.0
12	2057.0
13	3911.5
14	5766.0
15	9021.5
16	12277.0
17	11345.5
18	10414.0
19	10414.0
20	11150.5
21	11887.0
22	9939.5
23	7992.0
24	9079.0
25	10166.0
26	10166.0
27	11428.0
28	12690.0
29	13977.0
30	15264.0
31	17169.0
32	19074.0
33	19074.0
34	21084.5
35	23095.0
36	25091.5
37	27088.0
38	28915.5
39	30743.0
40	30743.0
41	31409.0
42	32075.0
43	33801.5
44	35528.0
45	36963.5
46	38399.0
47	38399.0
48	46679.5
49	54960.0
50	52460.0
51	49960.0
52	46461.0
53	42962.0
54	42962.0
55	38940.5
56	34919.0
57	30422.0
58	25925.0
59	23300.0
60	20675.0
61	20675.0
62	18287.5
63	15900.0
64	13856.0
65	11812.0
66	10196.0
67	8580.0
68	8580.0
69	7265.5
70	5951.0
71	5071.5
72	4192.0
73	3455.0
74	2718.0
75	2718.0
76	2217.0
77	1716.0
78	1370.0
79	1024.0
80	800.0
81	576.0
82	576.0
83	440.5
84	305.0
85	219.5
86	134.0
87	110.5
88	87.0
89	87.0
90	66.0
91	45.0
92	30.0
93	15.0
94	10.0
95	5.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	579102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.9971267103896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.12718282229132	59.7126516088511
2	6.510053290919668	8.723097304462675
3	1.6129532769777448	3.241897052268483
4	0.7366281645036423	1.9740788190276894
5	0.4298973525200031	1.4400943699621833
6	0.2648021347558999	1.0644589305253613
7	0.2016199411651314	0.9455569721907121
8	0.14929879183381622	0.8002072059358613
9	0.13098089401286245	0.7897809197537871
>10	0.7351747899318748	9.227540372376072
>50	0.06389773737010578	2.9741437181400934
>100	0.033112985351061934	4.2073029373823845
>500	0.002069561584440975	0.8993489162543715
>1k	0.002069561584440975	2.9791691118705708
>5k	2.5869519805512187E-4	1.0206717609986498
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5889	1.0169192991908163	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4991	0.8618516254476759	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4092	0.706611270553374	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2192	0.3785170833462844	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1277	0.22051383003339653	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1199	0.20704470024278968	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1179	0.2035910772195572	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1159	0.20013745419632467	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1100	0.18994926627778871	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	964	0.16646462971980758	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	640	0.1105159367434407	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	639	0.11034325559227907	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	636	0.1098252121387942	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	617	0.10654427026672329	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	604	0.10429941530162216	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	582	0.10050042997606638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.726811511616261E-4	0.0
9	0.0	0.0	0.0	0.0010360869069697565	0.0
10	0.0	0.0	0.0	0.0010360869069697565	0.0
11	0.0	0.0	0.0	0.0010360869069697565	0.0
12	0.0	0.0	0.0	0.0010360869069697565	0.0
13	0.0	0.0	0.0	0.0010360869069697565	0.0
14	0.0	0.0	0.0	0.0012087680581313828	0.0
15	0.0	0.0	0.0	0.0012087680581313828	0.0
16	0.0	0.0	0.0	0.002072173813939513	0.0
17	0.0	0.0	0.0	0.0027628984185860176	0.0
18	0.0	0.0	0.0	0.0027628984185860176	0.0
19	0.0	0.0	0.0	0.00310826072090927	0.0
20	0.0	0.0	0.0	0.004835072232525531	0.0
21	0.0	0.0	0.0	0.0079433329534348	0.0
22	0.0	0.0	0.0	0.012951086337121958	0.0
23	0.0	0.0	0.0	0.015713984755707976	0.0
24	0.0	0.0	0.0	0.020203694685910253	0.0
25	0.0	0.0	0.0	0.025384129220759038	0.0
26	0.0	0.0	0.0	0.03263673756954733	0.0
27	0.0	0.0	0.0	0.078051880325055	0.0
28	0.0	0.0	0.0	0.16491049935935292	0.0
29	0.0	0.0	0.0	0.2818156386957738	0.0
30	0.0	0.0	0.0	0.4413730223691163	0.0
31	0.0	0.0	0.0	0.604556710216853	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACCG	35	8.864059E-4	26.428572	5
GTCCGTG	40	0.0019300367	23.125	7
GGTATCA	1635	0.0	21.272171	1
CTAGTGA	45	0.0038235495	20.555557	29
GACCTAG	45	0.0038235495	20.555557	6
GTAAGAC	55	5.1388604E-4	20.181818	3
ACGGGCG	70	1.2178719E-4	18.5	5
CGTGTTT	50	0.0070307706	18.5	18
GAATTAG	60	9.229272E-4	18.5	1
TAGTACC	50	0.0070307706	18.5	4
TTATACC	85	2.71934E-5	17.411764	4
TTACACT	75	2.0650354E-4	17.266666	4
ACTGAGC	65	0.0015786545	17.076923	8
GGACCGT	120	1.0385338E-7	16.958334	6
CGGGTAA	80	3.3789416E-4	16.1875	26
CGTTATT	80	3.3789416E-4	16.1875	10
TTGGACC	275	0.0	16.145454	4
TGGACCC	255	0.0	15.960784	5
ATTATAC	70	0.0025904109	15.857143	3
TACACTT	70	0.0025904109	15.857143	5
>>END_MODULE