FastQCFastQC Report
Wed 25 May 2016
SRR2088469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713056
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT174891.0209240094894738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149420.8722423551827844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118940.6943147217604094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.3695734406814488No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG38260.22334354510302057No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG35670.20822436627874397No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA35440.20688173649898192No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA31900.18621691293221004No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31760.18539966002278968No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG29100.16987185474380287No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19400.11324790316253526No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.11178852296714176No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA18510.10805250966693442No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.10110585993686137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49100.023.6242371
GTATTGG6450.021.511631
GTATTAG4000.019.4251
ATTGGAC6850.019.1751823
GTACTAT1850.019.01
TATACCG1306.9667294E-1018.55
TTACCGG500.007036258518.49999831
TTGGACC8900.018.0842694
CCGTATA852.7243781E-517.4117642
CGTATAC752.0680117E-417.2666663
TATTAGA4500.017.2666662
CTCGCGG650.00158036217.07692310
TAACGGC3250.016.50769236
TTAACGG3250.016.50769235
CGCCTAT904.448004E-516.44444536
GGACCCT9000.016.4444456
TGGACCC9050.016.353595
TCTAATA4600.016.0869562
ATACTGG4300.015.9186046
CTCTAAT4300.015.9186041
Produced by FastQC (version 0.11.5)