##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1713056
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39484231688865	31.0	31.0	34.0	30.0	34.0
2	31.5290264883343	31.0	31.0	34.0	30.0	34.0
3	31.678264458371473	31.0	31.0	34.0	30.0	34.0
4	35.38887461939365	37.0	35.0	37.0	33.0	37.0
5	35.25140450750005	37.0	35.0	37.0	33.0	37.0
6	35.322657869912014	37.0	35.0	37.0	33.0	37.0
7	35.16084237759886	37.0	35.0	37.0	32.0	37.0
8	35.19974303233519	37.0	35.0	37.0	32.0	37.0
9	36.75324682905871	39.0	35.0	39.0	32.0	39.0
10	36.586246450787364	39.0	35.0	39.0	32.0	39.0
11	36.62040003362412	39.0	35.0	39.0	32.0	39.0
12	36.469775652401324	38.0	35.0	39.0	32.0	39.0
13	36.48912061251938	38.0	35.0	39.0	32.0	39.0
14	37.44721246707638	39.0	36.0	41.0	32.0	41.0
15	37.46684288196066	39.0	36.0	41.0	32.0	41.0
16	37.330974585769525	39.0	36.0	41.0	32.0	41.0
17	37.38776724170138	39.0	36.0	41.0	32.0	41.0
18	37.252386962247584	39.0	36.0	40.0	31.0	41.0
19	37.33650446920591	39.0	36.0	41.0	31.0	41.0
20	37.227644630414886	39.0	36.0	40.0	31.0	41.0
21	37.064801734444174	39.0	36.0	40.0	31.0	41.0
22	36.96122018194385	39.0	36.0	40.0	31.0	41.0
23	36.76019639754917	39.0	35.0	40.0	30.0	41.0
24	36.97448594791997	39.0	36.0	40.0	31.0	41.0
25	36.93954429977771	39.0	36.0	40.0	30.0	41.0
26	36.74206097173706	39.0	35.0	40.0	30.0	41.0
27	36.687858423775985	39.0	35.0	40.0	30.0	41.0
28	36.49106100442717	39.0	35.0	40.0	30.0	41.0
29	36.317797550109276	38.0	35.0	40.0	30.0	41.0
30	36.13568266303028	38.0	35.0	40.0	29.0	41.0
31	35.88420401901631	38.0	35.0	40.0	28.0	41.0
32	35.748402854314165	38.0	34.0	40.0	27.0	41.0
33	35.55124350867689	38.0	34.0	40.0	27.0	41.0
34	35.435810621485814	38.0	34.0	40.0	27.0	41.0
35	35.185646003399775	38.0	34.0	40.0	26.0	41.0
36	35.030583355126744	38.0	33.0	40.0	25.0	41.0
37	34.873243489996824	38.0	33.0	40.0	25.0	41.0
38	34.786190877589526	38.0	33.0	40.0	24.0	41.0
39	34.69282848896942	38.0	33.0	40.0	24.0	41.0
40	34.574010423477105	38.0	33.0	40.0	23.0	41.0
41	34.368257079745206	38.0	33.0	40.0	23.0	41.0
42	34.15452734761736	38.0	33.0	40.0	22.0	41.0
43	33.81122683671754	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	4.0
15	8.0
16	25.0
17	72.0
18	168.0
19	381.0
20	897.0
21	1697.0
22	3210.0
23	5331.0
24	8497.0
25	12763.0
26	17806.0
27	24567.0
28	32723.0
29	42813.0
30	53074.0
31	65402.0
32	79792.0
33	97401.0
34	117053.0
35	141894.0
36	178361.0
37	234024.0
38	302772.0
39	292316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.338461789923976	21.30058795509312	13.464825434778549	23.89612482020436
2	19.292539181439487	23.510498197373582	34.439738105467654	22.757224515719276
3	19.571280798759645	24.79171725851344	32.27413464591934	23.362867296807575
4	14.02370967440644	16.597822838249304	36.28217641454803	33.09629107279622
5	12.687734668335418	38.56587291950759	35.01333289746512	13.733059514691872
6	32.41943053817272	37.75696766480488	13.707316048045131	16.116285748977266
7	26.438423495787646	32.6597028935423	21.609392804438386	19.292480806231673
8	27.08755580669867	34.3226958137971	18.998795135710683	19.59095324379355
9	25.001167504156314	14.232634543178973	21.172454373937573	39.59374357872714
10	16.514754917527508	27.451058225767284	33.51268143014589	22.52150542655932
11	33.702809482001754	22.58653540806605	23.061826350101803	20.648828759830383
12	21.2630526964676	27.16846384473129	30.52801542973493	21.040468029066183
13	31.26027403657557	21.003166271271926	25.739789008648874	21.996770683503634
14	20.902118786542882	22.67357284291932	27.229874563353444	29.194433807184357
15	23.254172659854667	29.411706330674537	24.92837362001009	22.405747389460707
16	22.48350316627127	28.204682158668486	25.999675433844544	23.3121392412157
17	22.23949479760148	26.81184970018493	26.674551211402314	24.274104290811273
18	23.09685147479125	25.866930211271555	28.76555115536211	22.270667158575083
19	23.25212952758112	25.038469261950574	29.673869388975028	22.035531821493283
20	23.88929492088992	24.9306502531149	29.375922328283487	21.80413249771169
21	22.871231296583417	25.62858423775989	29.74146787962565	21.758716586031046
22	21.98188500551062	26.04304821325164	29.27685960062018	22.698207180617562
23	22.713676610688736	26.155011861842226	30.0576280051557	21.07368352231334
24	22.766097547307268	26.3028762632395	27.907669101302	23.023357088151233
25	22.704453327853848	26.14870733939813	29.10126697551043	22.045572357237592
26	22.368445631666447	26.88178319914819	29.020709188724712	21.72906198046065
27	21.650372200325034	26.917859077578317	28.603559953673436	22.828208768423217
28	21.946859880821176	26.62376478060262	28.848677451291728	22.58069788728448
29	22.467041357667235	26.563755066968035	28.345191283880972	22.62401229148376
30	22.24235518278445	26.747403470756353	29.64719191900323	21.363049427455962
31	22.653666897054155	26.545075000467	29.51018530625969	21.291072796219154
32	22.14901322548708	26.37426914239815	28.695909532437934	22.780808099676836
33	21.90914949657221	26.371700633254253	29.355666131171425	22.36348373900211
34	21.53846692694226	26.65768077634356	29.755477929501428	22.048374367212748
35	21.2137256458633	28.35925970896456	28.790243868268174	21.636770776903965
36	22.98640558160387	26.50794836829619	28.58347888218482	21.92216716791512
37	21.93325845740011	26.95586133786636	28.676704089066558	22.434176115666972
38	21.834370855360245	25.804585489324346	29.948174490501184	22.41286916481423
39	21.38114574187884	25.826184316216168	30.542434106065418	22.250235835839575
40	20.59821745465414	26.25033857620533	30.29935974072068	22.85208422841985
41	21.55300235368838	25.157496310686867	30.47868837913063	22.810812956494125
42	20.425893841182074	26.845240319055534	29.993181775727123	22.73568406403527
43	20.888108736667103	26.273396783292547	29.50884267647993	23.32965180356042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	206.0
2	296.0
3	669.5
4	1043.0
5	1043.0
6	1700.0
7	2357.0
8	2602.0
9	2847.0
10	4350.0
11	5853.0
12	5853.0
13	11112.0
14	16371.0
15	26107.5
16	35844.0
17	32911.0
18	29978.0
19	29978.0
20	32275.0
21	34572.0
22	28356.5
23	22141.0
24	24591.5
25	27042.0
26	27042.0
27	30445.0
28	33848.0
29	37258.5
30	40669.0
31	45816.5
32	50964.0
33	50964.0
34	58206.5
35	65449.0
36	71787.5
37	78126.0
38	84094.5
39	90063.0
40	90063.0
41	93306.5
42	96550.0
43	102935.5
44	109321.0
45	112676.5
46	116032.0
47	116032.0
48	141148.5
49	166265.0
50	159036.0
51	151807.0
52	140537.0
53	129267.0
54	129267.0
55	117136.5
56	105006.0
57	91476.5
58	77947.0
59	70004.0
60	62061.0
61	62061.0
62	55086.0
63	48111.0
64	41876.0
65	35641.0
66	30800.5
67	25960.0
68	25960.0
69	22305.5
70	18651.0
71	15702.0
72	12753.0
73	10530.0
74	8307.0
75	8307.0
76	6730.0
77	5153.0
78	4131.0
79	3109.0
80	2391.0
81	1673.0
82	1673.0
83	1303.0
84	933.0
85	691.5
86	450.0
87	339.5
88	229.0
89	229.0
90	193.0
91	157.0
92	108.0
93	59.0
94	42.5
95	26.0
96	26.0
97	16.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1713056.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.939989932712585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89315037207194	44.712556965603916
2	9.912890940179723	10.694024750347442
3	2.889726093973779	4.6761538925172745
4	1.1783109804172482	2.5423232968524427
5	0.6778287094489214	1.828103688188919
6	0.4295211245202534	1.3901019079505914
7	0.30205345410984324	1.1404932191678205
8	0.21660960530623594	0.934713594363777
9	0.17250908968565465	0.8374624704851064
>10	1.1429475620495617	11.947963662085108
>50	0.10439777906401826	3.9444926867902232
>100	0.07059159425177212	7.359055859444122
>500	0.006308463280793977	2.2863191439730937
>1k	0.002719165207238783	2.7343133185529895
>5k	1.0876660828955133E-4	0.37143152426209025
>10k+	3.2629982486865396E-4	2.600490019415124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17489	1.0209240094894738	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14942	0.8722423551827844	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11894	0.6943147217604094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6331	0.3695734406814488	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3826	0.22334354510302057	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3567	0.20822436627874397	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3544	0.20688173649898192	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3190	0.18621691293221004	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3176	0.18539966002278968	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2910	0.16987185474380287	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1940	0.11324790316253526	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1915	0.11178852296714176	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1851	0.10805250966693442	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1732	0.10110585993686137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	5.8375207815739825E-5	0.0	0.0
3	0.0	0.0	5.8375207815739825E-5	0.0	0.0
4	0.0	0.0	5.8375207815739825E-5	0.0	0.0
5	0.0	0.0	5.8375207815739825E-5	0.0	0.0
6	0.0	0.0	5.8375207815739825E-5	0.0	0.0
7	0.0	0.0	5.8375207815739825E-5	0.0	0.0
8	5.8375207815739825E-5	0.0	5.8375207815739825E-5	0.0	0.0
9	5.8375207815739825E-5	0.0	5.8375207815739825E-5	1.7512562344721948E-4	0.0
10	5.8375207815739825E-5	0.0	5.8375207815739825E-5	1.7512562344721948E-4	0.0
11	5.8375207815739825E-5	0.0	5.8375207815739825E-5	4.086264547101788E-4	0.0
12	5.8375207815739825E-5	0.0	5.8375207815739825E-5	5.253768703416584E-4	0.0
13	5.8375207815739825E-5	0.0	5.8375207815739825E-5	5.253768703416584E-4	0.0
14	1.1675041563147965E-4	0.0	5.8375207815739825E-5	8.172529094203576E-4	0.0
15	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.0011675041563147964	0.0
16	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.0015177554032092355	0.0
17	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.0016928810266564549	0.0
18	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.0019263818579194141	0.0
19	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.0025101339360768123	0.0
20	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.004203014962733267	0.0
21	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.0077055274316776565	0.0
22	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.013484673005435899	0.0
23	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.01722068630564325	0.0
24	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.021890702930902433	0.0
25	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.02556834102329404	0.0
26	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.03233986512991986	0.0
27	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.07833952888872284	0.0
28	1.1675041563147965E-4	0.0	5.8375207815739825E-5	0.17191498701735378	0.0
29	1.7512562344721948E-4	0.0	5.8375207815739825E-5	0.29602067883361666	0.0
30	1.7512562344721948E-4	0.0	5.8375207815739825E-5	0.46805241626660193	0.0
31	2.335008312629593E-4	0.0	5.8375207815739825E-5	0.66950525843872	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4910	0.0	23.624237	1
GTATTGG	645	0.0	21.51163	1
GTATTAG	400	0.0	19.425	1
ATTGGAC	685	0.0	19.175182	3
GTACTAT	185	0.0	19.0	1
TATACCG	130	6.9667294E-10	18.5	5
TTACCGG	50	0.0070362585	18.499998	31
TTGGACC	890	0.0	18.084269	4
CCGTATA	85	2.7243781E-5	17.411764	2
CGTATAC	75	2.0680117E-4	17.266666	3
TATTAGA	450	0.0	17.266666	2
CTCGCGG	65	0.001580362	17.076923	10
TAACGGC	325	0.0	16.507692	36
TTAACGG	325	0.0	16.507692	35
CGCCTAT	90	4.448004E-5	16.444445	36
GGACCCT	900	0.0	16.444445	6
TGGACCC	905	0.0	16.35359	5
TCTAATA	460	0.0	16.086956	2
ATACTGG	430	0.0	15.918604	6
CTCTAAT	430	0.0	15.918604	1
>>END_MODULE