FastQCFastQC Report
Wed 25 May 2016
SRR2088468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2609898
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT305931.1721914036487249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT252360.9669343399627112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT202400.7755092344605038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110450.42319661534665337No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61690.23636939068116838No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59640.22851467758510102No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA55440.21242209465657277No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA51630.19782382299997933No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA49150.1883215359374198No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG46790.17927903695853248No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA32880.12598193492619253No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.12456425500153645No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31050.11897016665019093No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28700.109965983344943No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28140.10782030562113921No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27440.10513820846638451No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.10100011571333438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84550.023.1277351
GTATTGG9800.019.0663281
TTGGACC14200.016.8063374
GGACCCT14150.016.3427546
TGGACCC14150.016.081275
ACCGTTA5150.015.8058268
ATTGGAC11350.015.6475773
TACCGTC957.06517E-515.5789487
GCACCGT2300.015.2826096
GTATCAA130100.015.0730212
CTAACCT1853.0559022E-1015.04
TATACTG4000.014.85
ATTAGAG5400.014.7314813
GACCGTT5700.014.2807027
AGAACCG1304.448886E-614.2307695
TATTGGA12800.014.16406252
CGTTTAC5400.014.04629528
ACCGTGG2800.013.8758
CCGTTTA5500.013.7909127
GTATTAG6050.013.760331
Produced by FastQC (version 0.11.5)