FastQCFastQC Report
Wed 25 May 2016
SRR2088467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800050
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT399951.4283673505830252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT346131.236156497205407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT276290.9867323797789325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146540.5233477973607614No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG97150.34695809003410655No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG89220.318637167193443No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86240.3079945000982125No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA80030.28581632470848733No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA79840.2851377653970465No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG69620.24863841717112195No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46290.16531847645577757No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA44290.1581757468616632No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.15710433742254604No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42580.15206871305869538No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC42340.15121158550740166No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.13224763843502793No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA36200.12928340565347046No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG33790.12067641649256262No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33700.12035499366082748No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG31870.11381939608221282No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31460.11235513651541937No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC30270.10810521240692131No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30030.10724808485562758No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28340.10121247834860092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104550.023.3926331
GTATTGG14550.019.0721651
ATTGGAC16300.017.5920243
ACCGTTA4700.016.5319168
TTGGACC20200.016.3019814
TATACTG3200.016.18755
TTCGTTA2650.016.05660430
TTAACGG2900.015.94827635
TATTGGA17650.015.4079312
CGTTCGC3150.015.26984123
GTATTAG5000.015.1700011
ACGTTCG3050.015.16393522
CCGTTTA5050.015.01980227
GACCGTT5250.014.7999997
TAGTACT5250.014.7999994
GTATCAA165950.014.748722
TGGACCC21300.014.6784045
CCGTTAT5250.014.44761859
ATTAGAG4900.014.3469383
CGCCGTT5350.014.17757125
Produced by FastQC (version 0.11.5)