##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088467_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2800050
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409477330761952	31.0	31.0	34.0	30.0	34.0
2	31.53179728933412	31.0	31.0	34.0	30.0	34.0
3	31.66728094141176	31.0	31.0	34.0	30.0	34.0
4	35.366168104140996	37.0	35.0	37.0	33.0	37.0
5	35.25006124890627	37.0	35.0	37.0	33.0	37.0
6	35.33602578525384	37.0	35.0	37.0	33.0	37.0
7	35.160826413814036	37.0	35.0	37.0	32.0	37.0
8	35.22448349136623	37.0	35.0	37.0	32.0	37.0
9	36.75584114569383	39.0	37.0	39.0	32.0	39.0
10	36.61704076712916	39.0	35.0	39.0	32.0	39.0
11	36.64795307226657	39.0	35.0	39.0	32.0	39.0
12	36.4951447295584	38.0	35.0	39.0	32.0	39.0
13	36.523293155479365	38.0	35.0	39.0	32.0	39.0
14	37.44486491312655	39.0	36.0	41.0	32.0	41.0
15	37.475769718397885	39.0	36.0	41.0	32.0	41.0
16	37.333117265763114	39.0	36.0	41.0	32.0	41.0
17	37.427238799307155	39.0	36.0	41.0	32.0	41.0
18	37.29891751933001	39.0	36.0	40.0	32.0	41.0
19	37.390411242656384	39.0	36.0	41.0	32.0	41.0
20	37.29914251531223	39.0	36.0	41.0	31.0	41.0
21	37.14902983875288	39.0	36.0	40.0	31.0	41.0
22	37.04700880341422	39.0	36.0	40.0	31.0	41.0
23	36.83973536186854	39.0	35.0	40.0	30.0	41.0
24	37.04188139497509	39.0	36.0	40.0	31.0	41.0
25	37.00217996107212	39.0	36.0	41.0	31.0	41.0
26	36.79551186585954	39.0	35.0	40.0	30.0	41.0
27	36.72937840395707	39.0	35.0	40.0	30.0	41.0
28	36.52378636095784	39.0	35.0	40.0	30.0	41.0
29	36.346142033177976	38.0	35.0	40.0	30.0	41.0
30	36.14946661666756	38.0	35.0	40.0	30.0	41.0
31	35.85378296816128	38.0	35.0	40.0	29.0	41.0
32	35.70673523687077	38.0	34.0	40.0	28.0	41.0
33	35.493244763486366	38.0	34.0	40.0	27.0	41.0
34	35.364942411742646	38.0	34.0	40.0	27.0	41.0
35	35.10128783414582	38.0	34.0	40.0	25.0	41.0
36	34.92612703344583	38.0	33.0	40.0	25.0	41.0
37	34.75780932483349	38.0	33.0	40.0	24.0	41.0
38	34.63665791682291	38.0	33.0	40.0	24.0	41.0
39	34.51424260281066	38.0	33.0	40.0	23.0	41.0
40	34.34372886198461	38.0	33.0	40.0	23.0	41.0
41	34.09745790253746	38.0	33.0	40.0	21.0	41.0
42	33.829215906858806	37.0	33.0	40.0	20.0	41.0
43	33.479341083194946	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	7.0
14	13.0
15	25.0
16	38.0
17	126.0
18	286.0
19	682.0
20	1354.0
21	2748.0
22	5180.0
23	8719.0
24	13758.0
25	20844.0
26	29529.0
27	40919.0
28	54811.0
29	70293.0
30	87545.0
31	107731.0
32	130686.0
33	159321.0
34	192711.0
35	233147.0
36	294952.0
37	389942.0
38	482760.0
39	471919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70257673970107	21.256477562900663	14.08299851788361	22.95794717951465
2	19.396475062945303	23.876252209781967	34.402492812628346	22.32477991464438
3	20.061106051677648	24.38767164872056	32.96326851306227	22.58795378653953
4	13.97192907269513	16.219996071498723	35.670970161247126	34.13710469455903
5	12.401099980357493	38.844413492616205	34.67059516794343	14.083891359082873
6	32.58423956715058	36.89659113230121	12.97716112212282	17.542008178425387
7	26.096569704112426	33.1809431974429	20.804092784057428	19.918394314387243
8	27.81936036856485	33.83211014089034	18.45392046570597	19.894609024838843
9	24.524562061391762	14.245888466277387	21.65607757004339	39.57347190228746
10	16.798200032142283	27.530686952018712	32.760914983661	22.910198032178
11	33.198300030356606	22.269495187585935	23.385082409242692	21.14712237281477
12	21.511008731986927	27.301548186639522	30.48406278459313	20.703380296780416
13	32.40406421313905	20.61688184139569	26.07367725576329	20.905376689701967
14	20.621060338208245	23.1239085016339	27.612042642095673	28.64298851806218
15	22.68491634077963	29.415689005553475	25.62554240102855	22.273852252638346
16	21.847217013981894	28.170925519187158	26.526276316494346	23.455581150336602
17	21.99314297958965	26.490741236763633	26.65052409778397	24.865591685862753
18	23.45486687737719	24.999517865752395	29.444009928394138	22.101605328476275
19	23.059873930822665	24.225245977750397	30.80005714183675	21.914822949590185
20	24.097819681791396	23.834145818824666	30.607239156443633	21.460795342940305
21	22.891484080641415	24.698201817824682	31.30683380653917	21.103480294994732
22	21.274084391350154	25.221835324369206	30.78937876109355	22.714701523187088
23	22.47081302119605	25.438152890126965	31.78318244317066	20.307851645506332
24	22.319387153800825	25.512580132497636	29.05198121462117	23.116051499080374
25	22.21353190121605	25.809074837949318	30.323279941429615	21.65411331940501
26	22.085177050409815	26.520704987410937	30.262316744343853	21.131801217835395
27	20.799735719005017	26.680952125854894	29.877180764629202	22.642131390510883
28	21.437581471759433	26.112497991107304	29.947965214906873	22.50195532222639
29	21.669434474384385	26.040856413278334	29.29861966750594	22.991089444831342
30	21.6509705183836	26.32352993696541	31.16647917001482	20.859020374636167
31	22.456241852824057	26.048463420296063	30.861591757290046	20.63370296958983
32	22.089391260870343	25.909930179818218	29.616256852556205	22.384421706755237
33	21.59293584043142	25.881037838610027	30.438420742486745	22.087605578471813
34	20.702737451116946	26.687487723433513	30.906948090212676	21.702826735236872
35	20.235138658238245	29.01898180389636	29.72975482580668	21.016124712058712
36	22.773664755986502	26.247709862323887	29.578293244763486	21.400332136926124
37	21.636899341083197	27.138801092837628	29.51283012803343	21.71146943804575
38	21.450295530436954	25.487116301494616	31.069623756718627	21.992964411349796
39	20.986339529651257	25.251870502312457	31.794182246745596	21.96760772129069
40	19.770075534365457	25.984250281244982	31.346225960250713	22.899448224138855
41	21.311333726183463	24.89355547222371	31.3525472759415	22.442563525651327
42	19.955536508276637	26.948590203746363	30.75237942179604	22.34349386618096
43	20.304887412724774	26.826806664166714	29.913715826503097	22.95459009660542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	362.0
2	552.0
3	1286.0
4	2020.0
5	2020.0
6	3275.5
7	4531.0
8	5142.0
9	5753.0
10	8974.5
11	12196.0
12	12196.0
13	23910.5
14	35625.0
15	57555.5
16	79486.0
17	71783.0
18	64080.0
19	64080.0
20	67816.5
21	71553.0
22	54879.5
23	38206.0
24	41689.5
25	45173.0
26	45173.0
27	48965.0
28	52757.0
29	56792.5
30	60828.0
31	66585.0
32	72342.0
33	72342.0
34	81044.5
35	89747.0
36	98946.5
37	108146.0
38	116040.0
39	123934.0
40	123934.0
41	129249.5
42	134565.0
43	145542.0
44	156519.0
45	170964.0
46	185409.0
47	185409.0
48	249640.5
49	313872.0
50	294214.5
51	274557.0
52	251798.5
53	229040.0
54	229040.0
55	202717.0
56	176394.0
57	150218.0
58	124042.0
59	110017.0
60	95992.0
61	95992.0
62	84742.0
63	73492.0
64	64014.0
65	54536.0
66	46570.0
67	38604.0
68	38604.0
69	33108.5
70	27613.0
71	23312.0
72	19011.0
73	15808.5
74	12606.0
75	12606.0
76	10095.5
77	7585.0
78	5864.5
79	4144.0
80	3215.0
81	2286.0
82	2286.0
83	1780.0
84	1274.0
85	973.5
86	673.0
87	503.5
88	334.0
89	334.0
90	291.5
91	249.0
92	166.5
93	84.0
94	66.0
95	48.0
96	48.0
97	30.5
98	13.0
99	10.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2800050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.03097422425367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7404166490909	36.24498089181298
2	11.691247917220108	10.763190630538363
3	3.7719882720291262	5.208848847718795
4	1.742029697392409	3.207492963942176
5	0.9382246412146272	2.1593697138155075
6	0.5771397974019957	1.593978428280135
7	0.38229647307898623	1.231823536882533
8	0.2933458496847749	1.0802396196505333
9	0.2316644103858074	0.9597364652831422
>10	1.388264929246491	12.25884798586971
>50	0.14015773547136964	4.425219571092998
>100	0.0904780525107647	7.958777723552004
>500	0.006839089170713111	2.1036458585243842
>1k	0.005129316878033016	4.826000565479164
>5k	4.663015343666378E-4	1.796205335631593
>10k+	3.1086768957775855E-4	4.181641861926161
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39995	1.4283673505830252	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34613	1.236156497205407	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27629	0.9867323797789325	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14654	0.5233477973607614	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9715	0.34695809003410655	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8922	0.318637167193443	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8624	0.3079945000982125	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8003	0.28581632470848733	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7984	0.2851377653970465	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6962	0.24863841717112195	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4629	0.16531847645577757	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4429	0.1581757468616632	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4399	0.15710433742254604	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4258	0.15206871305869538	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4234	0.15121158550740166	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3703	0.13224763843502793	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3620	0.12928340565347046	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3379	0.12067641649256262	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3370	0.12035499366082748	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3187	0.11381939608221282	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3146	0.11235513651541937	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3027	0.10810521240692131	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3003	0.10724808485562758	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2834	0.10121247834860092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.5713647970571955E-5	0.0	0.0	0.0	0.0
3	3.5713647970571955E-5	0.0	0.0	0.0	0.0
4	3.5713647970571955E-5	0.0	0.0	3.5713647970571955E-5	0.0
5	3.5713647970571955E-5	0.0	0.0	7.142729594114391E-5	0.0
6	7.142729594114391E-5	0.0	0.0	7.142729594114391E-5	0.0
7	1.0714094391171586E-4	0.0	0.0	7.142729594114391E-5	0.0
8	1.0714094391171586E-4	0.0	0.0	7.142729594114391E-5	0.0
9	1.0714094391171586E-4	0.0	0.0	2.1428188782343172E-4	0.0
10	1.7856823985285976E-4	0.0	0.0	3.214228317351476E-4	0.0
11	1.7856823985285976E-4	0.0	0.0	4.999910715880073E-4	0.0
12	1.7856823985285976E-4	0.0	0.0	6.428456634702952E-4	0.0
13	1.7856823985285976E-4	0.0	0.0	8.571275512937269E-4	0.0
14	4.2856377564686344E-4	0.0	0.0	9.999821431760146E-4	0.0
15	4.2856377564686344E-4	0.0	0.0	0.0013214049749111622	0.0
16	4.999910715880073E-4	0.0	0.0	0.0018213960464991696	0.0
17	4.999910715880073E-4	0.0	0.0	0.0026428099498223244	0.0
18	4.999910715880073E-4	0.0	0.0	0.003178514669380904	0.0
19	4.999910715880073E-4	0.0	0.0	0.004107069516615775	0.0
20	4.999910715880073E-4	0.0	0.0	0.006749879466438099	0.0
21	4.999910715880073E-4	0.0	0.0	0.012106926662023893	0.0
22	5.357047195585793E-4	0.0	0.0	0.01942822449599114	0.0
23	5.357047195585793E-4	0.0	0.0	0.023892430492312638	0.0
24	5.357047195585793E-4	0.0	0.0	0.03003517794325101	0.0
25	5.357047195585793E-4	0.0	0.0	0.03503508865913109	0.0
26	5.357047195585793E-4	0.0	0.0	0.0445706326672738	0.0
27	5.357047195585793E-4	0.0	3.5713647970571955E-5	0.10335529722683523	0.0
28	5.357047195585793E-4	0.0	3.5713647970571955E-5	0.2174961161407832	0.0
29	5.714183675291513E-4	0.0	3.5713647970571955E-5	0.3633149408046285	0.0
30	6.071320154997232E-4	0.0	3.5713647970571955E-5	0.5703826717380047	0.0
31	6.071320154997232E-4	0.0	3.5713647970571955E-5	0.7909858752522276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10455	0.0	23.392633	1
GTATTGG	1455	0.0	19.072165	1
ATTGGAC	1630	0.0	17.592024	3
ACCGTTA	470	0.0	16.531916	8
TTGGACC	2020	0.0	16.301981	4
TATACTG	320	0.0	16.1875	5
TTCGTTA	265	0.0	16.056604	30
TTAACGG	290	0.0	15.948276	35
TATTGGA	1765	0.0	15.407931	2
CGTTCGC	315	0.0	15.269841	23
GTATTAG	500	0.0	15.170001	1
ACGTTCG	305	0.0	15.163935	22
CCGTTTA	505	0.0	15.019802	27
GACCGTT	525	0.0	14.799999	7
TAGTACT	525	0.0	14.799999	4
GTATCAA	16595	0.0	14.74872	2
TGGACCC	2130	0.0	14.678404	5
CCGTTAT	525	0.0	14.4476185	9
ATTAGAG	490	0.0	14.346938	3
CGCCGTT	535	0.0	14.177571	25
>>END_MODULE