FastQCFastQC Report
Wed 25 May 2016
SRR2088466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461484
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT198491.3581400822725394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160081.095325025795698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT131610.9005230300160658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74220.5078399763528031No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG38350.2624045148629749No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG37360.25563057823417845No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA34180.23387187269925638No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32480.22223986030637352No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA31950.21861340938388651No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG30700.21006045909500204No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.14731601577574577No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA20570.1407473499538825No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18920.129457455572555No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC18300.12521519222926833No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17870.12227297732989209No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17220.11782544317967217No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.11159889536936429No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA15050.10297752147816876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.022.5372491
TAAGCGA450.003826297420.5555575
CCGTATA701.2194851E-418.52
TAAGAGT1103.853711E-818.54
CGTATAC500.007035774618.4999983
TAGCGAA2500.017.0210
GGACCCT9150.016.9836066
GTTATCG904.447285E-516.44444511
GTCGGGT1801.0913936E-1116.44444524
TTCGTTA1352.2206223E-816.44444330
GTATTGG6800.016.323531
GTTCCGA1708.54925E-1116.3235333
TTGGACC9000.016.0333334
TCGGGTA1851.8189894E-1116.025
GTATTAG3150.015.8571431
TAATACT3650.015.7123294
CTTAGAC957.061772E-515.5789483
CTCTAAT3800.015.5789481
ACCGTTA2750.015.4727278
TGGACCC9700.015.2577325
Produced by FastQC (version 0.11.5)