##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1461484
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.392736424073064	31.0	31.0	34.0	30.0	34.0
2	31.52266873944566	31.0	31.0	34.0	30.0	34.0
3	31.66196619326657	31.0	31.0	34.0	30.0	34.0
4	35.368176456259526	37.0	35.0	37.0	33.0	37.0
5	35.235765153775205	37.0	35.0	37.0	33.0	37.0
6	35.32164430127186	37.0	35.0	37.0	33.0	37.0
7	35.15333524007105	37.0	35.0	37.0	32.0	37.0
8	35.20281918926242	37.0	35.0	37.0	32.0	37.0
9	36.73813397888721	39.0	35.0	39.0	32.0	39.0
10	36.59056548001894	39.0	35.0	39.0	32.0	39.0
11	36.62713379003807	39.0	35.0	39.0	32.0	39.0
12	36.47766722044169	38.0	35.0	39.0	32.0	39.0
13	36.50708457978329	38.0	35.0	39.0	32.0	39.0
14	37.442733550281766	39.0	36.0	41.0	32.0	41.0
15	37.47475921734347	39.0	36.0	41.0	32.0	41.0
16	37.331077863322484	39.0	36.0	41.0	32.0	41.0
17	37.41672300209924	39.0	36.0	41.0	32.0	41.0
18	37.29433302040939	39.0	36.0	40.0	32.0	41.0
19	37.386916312460485	39.0	36.0	41.0	32.0	41.0
20	37.28935725604933	39.0	36.0	41.0	31.0	41.0
21	37.14138642639947	39.0	36.0	40.0	31.0	41.0
22	37.030848096865924	39.0	36.0	40.0	31.0	41.0
23	36.833118939379425	39.0	35.0	40.0	30.0	41.0
24	37.039452364856544	39.0	36.0	40.0	31.0	41.0
25	36.9992124443374	39.0	36.0	41.0	31.0	41.0
26	36.79059572325116	39.0	35.0	40.0	30.0	41.0
27	36.722978835211336	39.0	35.0	40.0	30.0	41.0
28	36.519264665230686	39.0	35.0	40.0	30.0	41.0
29	36.33632047973156	38.0	35.0	40.0	30.0	41.0
30	36.136630301802825	38.0	35.0	40.0	30.0	41.0
31	35.864267415859494	38.0	35.0	40.0	29.0	41.0
32	35.71514228003865	38.0	34.0	40.0	28.0	41.0
33	35.5091010233434	38.0	34.0	40.0	27.0	41.0
34	35.38925366271543	38.0	34.0	40.0	27.0	41.0
35	35.13174622506986	38.0	34.0	40.0	26.0	41.0
36	34.95231490731339	38.0	33.0	40.0	25.0	41.0
37	34.785192995612675	38.0	33.0	40.0	24.0	41.0
38	34.6668612177759	38.0	33.0	40.0	24.0	41.0
39	34.54592729034324	38.0	33.0	40.0	23.0	41.0
40	34.39464886375766	38.0	33.0	40.0	23.0	41.0
41	34.16176365940373	38.0	33.0	40.0	21.0	41.0
42	33.91735181500447	38.0	33.0	40.0	20.0	41.0
43	33.55281754709596	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	5.0
15	7.0
16	26.0
17	56.0
18	141.0
19	295.0
20	742.0
21	1436.0
22	2703.0
23	4407.0
24	7062.0
25	10747.0
26	15363.0
27	21277.0
28	28395.0
29	36596.0
30	45817.0
31	56742.0
32	68071.0
33	83782.0
34	101003.0
35	121919.0
36	153654.0
37	202027.0
38	251531.0
39	247678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1600811230229	21.611047401134737	14.233067211136078	22.995804264706283
2	19.25631755120138	23.84788338428611	34.553440201876995	22.342358862635514
3	19.817459513754514	24.726852979574186	32.80268548954351	22.653002017127797
4	14.143774410120125	16.557348558041003	35.743326646066606	33.55555038577227
5	12.421278645541108	39.00877464276037	34.61454247874079	13.955404232957733
6	32.410891942710286	37.34669691902204	13.36607174625244	16.87633939201524
7	25.9131129728413	33.19468430718366	21.268860965977048	19.62334175399799
8	27.329413117078253	34.34700619370448	18.719602814673305	19.603977874543958
9	24.634823234465788	14.442306587003348	21.656891214683156	39.2659789638477
10	16.779998959961244	27.83910053069346	32.87952519493884	22.501375314406452
11	33.11948676824378	22.788959714919905	23.07887051791193	21.012682998924383
12	21.13536651786814	27.703827068924465	30.496946938864877	20.663859474342516
13	31.622515196882073	21.092328072014475	25.978115395036827	21.30704133606663
14	20.488489781619233	23.376239493555865	27.773003330860963	28.36226739396394
15	22.72642054240758	29.850959709446016	25.554436449526648	21.86818329861976
16	21.610978977532426	28.41173765843485	26.72400108382986	23.25328228020286
17	21.775811435499808	27.033960002299036	27.02410700356624	24.16612155863492
18	22.861694004176577	25.70147877089315	29.486535603537227	21.95029162139305
19	22.87264178054635	25.09743520969097	30.607930021813445	21.421992987949235
20	23.59984782590846	24.76010685029737	30.46779848428036	21.172246839513807
21	22.505343883340494	25.55156265822958	30.9431372495354	20.99995620889452
22	21.21439577853743	26.038465012275193	30.49509950160248	22.252039707584892
23	22.117176787429763	26.162517003265172	31.375779686948334	20.34452652235673
24	22.140440812215527	26.287595348289823	28.973427009806468	22.59853682968818
25	21.907937411562493	26.512914270700193	30.098926844221353	21.48022147351596
26	21.658122839524758	27.149732737409373	30.18404580549633	21.00809861756954
27	20.893831201710043	27.131395211990007	29.692080104879697	22.282693481420253
28	21.208100807124815	26.765807904841925	29.91206198630981	22.114029301723452
29	21.57998308568551	26.750891559538115	29.321497874762915	22.347627480013465
30	21.49801161011684	26.87466985611885	30.960653691727043	20.66666484203727
31	21.99983030946627	26.686162831751837	30.686275046459627	20.62773181232227
32	21.532360258477002	26.522561998626053	29.739360814076647	22.205716928820294
33	21.166704527726612	26.53049913649414	30.407517290644304	21.895279045134945
34	20.595572719236063	26.93454050814104	30.92931568186857	21.54057109075433
35	20.282534738662893	28.770482605351823	29.853491382731523	21.09349127325376
36	22.338869258917647	26.583527428285226	29.76987774070739	21.30772557208974
37	21.269818896409404	27.256952522230826	29.67559001672273	21.79763856463704
38	21.112239340286994	25.836341691048275	31.052546589630815	21.998872379033912
39	20.612747043416142	25.792345314762255	31.782900120699235	21.812007521122368
40	19.589472070854008	26.41370004734913	31.442561122803948	22.554266758992913
41	20.843060888795225	25.36394514069261	31.504279212088537	22.28871475842363
42	19.780647615711153	27.12865826789756	30.87170300872264	22.218991107668643
43	20.065563495734473	26.96300472670245	30.119248654107743	22.85218312345534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	204.0
2	287.0
3	678.5
4	1070.0
5	1070.0
6	1691.5
7	2313.0
8	2721.5
9	3130.0
10	4811.5
11	6493.0
12	6493.0
13	12687.5
14	18882.0
15	29845.5
16	40809.0
17	36597.0
18	32385.0
19	32385.0
20	33943.5
21	35502.0
22	28103.5
23	20705.0
24	22757.5
25	24810.0
26	24810.0
27	27261.5
28	29713.0
29	32074.0
30	34435.0
31	38558.5
32	42682.0
33	42682.0
34	47693.0
35	52704.0
36	57972.0
37	63240.0
38	67547.5
39	71855.0
40	71855.0
41	74242.5
42	76630.0
43	82358.0
44	88086.0
45	93522.5
46	98959.0
47	98959.0
48	125780.0
49	152601.0
50	142711.0
51	132821.0
52	121776.0
53	110731.0
54	110731.0
55	98916.0
56	87101.0
57	74997.0
58	62893.0
59	55820.0
60	48747.0
61	48747.0
62	43084.5
63	37422.0
64	32246.0
65	27070.0
66	23333.5
67	19597.0
68	19597.0
69	16803.0
70	14009.0
71	11739.5
72	9470.0
73	7746.5
74	6023.0
75	6023.0
76	4867.0
77	3711.0
78	2909.0
79	2107.0
80	1641.0
81	1175.0
82	1175.0
83	867.5
84	560.0
85	445.5
86	331.0
87	245.0
88	159.0
89	159.0
90	123.5
91	88.0
92	63.0
93	38.0
94	26.5
95	15.0
96	15.0
97	8.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1461484.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.625820977952635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.50605167399434	45.61586629319277
2	9.646567768763633	10.539033679763406
3	2.7269452172769726	4.468848637669682
4	1.149805215367942	2.5123621541682186
5	0.634496543269948	1.7329947291896977
6	0.41382934922969433	1.3563460767866353
7	0.2904740731545233	1.1107169303212994
8	0.2133300740661946	0.9322664348122659
9	0.16650596590348277	0.8185972576684261
>10	1.0795754974799205	11.1524910768424
>50	0.0968059514527733	3.720129894859503
>100	0.06668010004029806	6.837697991189495
>500	0.005284055493148774	2.0355719061775144
>1k	0.0031452711268742696	3.288226254193907
>5k	1.258108450749708E-4	0.5100784863651544
>10k+	3.774325352249124E-4	3.368772196799669
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19849	1.3581400822725394	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16008	1.095325025795698	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13161	0.9005230300160658	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7422	0.5078399763528031	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3835	0.2624045148629749	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3736	0.25563057823417845	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3418	0.23387187269925638	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3248	0.22223986030637352	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3195	0.21861340938388651	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3070	0.21006045909500204	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2153	0.14731601577574577	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2057	0.1407473499538825	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1892	0.129457455572555	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1830	0.12521519222926833	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1787	0.12227297732989209	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1722	0.11782544317967217	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1631	0.11159889536936429	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1505	0.10297752147816876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.842360231107559E-5	0.0	0.0	0.0	0.0
3	6.842360231107559E-5	0.0	0.0	0.0	0.0
4	6.842360231107559E-5	0.0	0.0	6.842360231107559E-5	0.0
5	6.842360231107559E-5	0.0	0.0	1.3684720462215117E-4	0.0
6	6.842360231107559E-5	0.0	0.0	1.3684720462215117E-4	0.0
7	6.842360231107559E-5	0.0	0.0	1.3684720462215117E-4	0.0
8	6.842360231107559E-5	0.0	0.0	1.3684720462215117E-4	0.0
9	6.842360231107559E-5	0.0	0.0	4.1054161386645354E-4	0.0
10	6.842360231107559E-5	0.0	0.0	4.7896521617752914E-4	0.0
11	6.842360231107559E-5	0.0	0.0	5.473888184886047E-4	0.0
12	6.842360231107559E-5	0.0	0.0	7.526596254218315E-4	0.0
13	1.3684720462215117E-4	0.0	0.0	0.0010263540346661338	0.0
14	1.3684720462215117E-4	0.0	0.0	0.0012316248415993607	0.0
15	2.0527080693322677E-4	0.0	0.0	0.0013684720462215118	0.0
16	2.0527080693322677E-4	0.0	0.0	0.0024632496831987214	0.0
17	2.7369440924430234E-4	0.0	0.0	0.0036264509224870063	0.0
18	2.7369440924430234E-4	0.0	0.0	0.004105416138664535	0.0
19	2.7369440924430234E-4	0.0	0.0	0.005131770173330669	0.0
20	2.7369440924430234E-4	0.0	0.0	0.008005561470395843	0.0
21	2.7369440924430234E-4	0.0	0.0	0.01286363723448221	0.0
22	2.7369440924430234E-4	0.0	0.0	0.02080077510256698	0.0
23	2.7369440924430234E-4	0.0	0.0	0.026890475708252707	0.0
24	2.7369440924430234E-4	0.0	0.0	0.03523815519020393	0.0
25	2.7369440924430234E-4	0.0	0.0	0.04002780735197922	0.0
26	2.7369440924430234E-4	0.0	0.0	0.049949229687085185	0.0
27	2.7369440924430234E-4	0.0	0.0	0.10653554879834469	0.0
28	2.7369440924430234E-4	0.0	0.0	0.23195601183454626	0.0
29	2.7369440924430234E-4	0.0	0.0	0.39035665118468627	0.0
30	2.7369440924430234E-4	0.0	0.0	0.6123912406841265	0.0
31	2.7369440924430234E-4	0.0	0.0	0.8747273319447904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5705	0.0	22.537249	1
TAAGCGA	45	0.0038262974	20.555557	5
CCGTATA	70	1.2194851E-4	18.5	2
TAAGAGT	110	3.853711E-8	18.5	4
CGTATAC	50	0.0070357746	18.499998	3
TAGCGAA	250	0.0	17.02	10
GGACCCT	915	0.0	16.983606	6
GTTATCG	90	4.447285E-5	16.444445	11
GTCGGGT	180	1.0913936E-11	16.444445	24
TTCGTTA	135	2.2206223E-8	16.444443	30
GTATTGG	680	0.0	16.32353	1
GTTCCGA	170	8.54925E-11	16.32353	33
TTGGACC	900	0.0	16.033333	4
TCGGGTA	185	1.8189894E-11	16.0	25
GTATTAG	315	0.0	15.857143	1
TAATACT	365	0.0	15.712329	4
CTTAGAC	95	7.061772E-5	15.578948	3
CTCTAAT	380	0.0	15.578948	1
ACCGTTA	275	0.0	15.472727	8
TGGACCC	970	0.0	15.257732	5
>>END_MODULE