FastQCFastQC Report
Wed 25 May 2016
SRR2088465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221879
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT247321.113111920136065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210060.9454160195042125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT153810.6922519183087829No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89730.40384737422694933No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG55640.2504186771646881No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA49410.22237934649006538No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG49080.22089411709638554No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45750.2059068023056161No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA44360.19965083607163125No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG35450.1595496424422752No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26560.11953846271556642No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25810.11616294136629403No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.11125718367201813No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA22470.10113061962420096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.023.824671
TTCGTTA1705.456968E-1217.41176430
CGTTAAC650.00158056117.07692310
GTATCAA95550.016.0120352
CTAATAC5550.015.9999993
TAATACT6350.015.7322854
GTATTAG5500.015.1363631
GTATTGG8200.014.8902441
ACGTTCG1752.240995E-914.822
TTAACGG3150.014.68253935
TTAGACT2550.014.5098044
ACGGGTA1956.730261E-1014.230775
TTGGACC13000.014.2307694
CTCTAAT4450.014.1348321
ATTGGAC11050.014.0633483
GTACACT1356.5731E-613.7037044
TCTATAC2051.4260877E-913.5365853
TGCGGTA1652.5980626E-713.45454536
CGTTCGC2202.8558134E-1013.45454523
TACCCCG4000.013.41255
Produced by FastQC (version 0.11.5)