##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2221879
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125810181382516	31.0	31.0	33.0	30.0	34.0
2	31.273536497712072	31.0	31.0	34.0	30.0	34.0
3	31.400870614466406	31.0	31.0	34.0	28.0	34.0
4	35.15371089064706	35.0	35.0	37.0	33.0	37.0
5	34.98913532195048	35.0	35.0	37.0	32.0	37.0
6	35.086292727911825	36.0	35.0	37.0	32.0	37.0
7	34.91836774189774	36.0	35.0	37.0	32.0	37.0
8	34.965414408255356	36.0	35.0	37.0	32.0	37.0
9	36.470741205979266	38.0	35.0	39.0	32.0	39.0
10	36.27248063463402	38.0	35.0	39.0	32.0	39.0
11	36.32036037966064	38.0	35.0	39.0	32.0	39.0
12	36.15017694482913	38.0	35.0	39.0	31.0	39.0
13	36.19223639091057	38.0	35.0	39.0	32.0	39.0
14	37.07845386719979	39.0	36.0	40.0	31.0	41.0
15	37.10382113517433	39.0	36.0	40.0	31.0	41.0
16	36.94716138907654	38.0	36.0	40.0	31.0	41.0
17	37.01127424130657	38.0	36.0	40.0	31.0	41.0
18	36.87516691953072	38.0	36.0	40.0	31.0	41.0
19	36.958715573620346	39.0	36.0	40.0	31.0	41.0
20	36.85713353427437	39.0	36.0	40.0	31.0	41.0
21	36.69411340581553	39.0	35.0	40.0	30.0	41.0
22	36.581332736841205	38.0	35.0	40.0	30.0	41.0
23	36.37843824978768	38.0	35.0	40.0	30.0	41.0
24	36.5996294127628	38.0	35.0	40.0	30.0	41.0
25	36.56230109740449	38.0	35.0	40.0	30.0	41.0
26	36.336877030657384	38.0	35.0	40.0	30.0	41.0
27	36.26778911002804	38.0	35.0	40.0	30.0	41.0
28	36.06140658424694	38.0	35.0	40.0	29.0	41.0
29	35.89096750993191	38.0	34.0	40.0	28.0	41.0
30	35.72182508588452	38.0	34.0	40.0	27.0	41.0
31	35.44562777721019	38.0	34.0	40.0	27.0	41.0
32	35.30545182703469	38.0	34.0	40.0	26.0	41.0
33	35.11313307340319	38.0	33.0	40.0	26.0	41.0
34	34.991325360201884	38.0	33.0	40.0	25.0	41.0
35	34.73669268218476	38.0	33.0	40.0	25.0	41.0
36	34.595748913419676	38.0	33.0	40.0	24.0	41.0
37	34.4442442635265	37.0	33.0	40.0	24.0	41.0
38	34.348209781000676	37.0	33.0	40.0	24.0	41.0
39	34.25494727660687	37.0	33.0	40.0	23.0	41.0
40	34.12681023584093	37.0	33.0	40.0	23.0	41.0
41	33.90427471522977	37.0	33.0	40.0	21.0	41.0
42	33.6835885302485	37.0	32.0	40.0	20.0	41.0
43	33.34357676543142	37.0	31.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	3.0
14	13.0
15	16.0
16	41.0
17	135.0
18	331.0
19	707.0
20	1435.0
21	2905.0
22	5115.0
23	8551.0
24	13006.0
25	19554.0
26	27432.0
27	37468.0
28	49502.0
29	62239.0
30	77705.0
31	94416.0
32	112995.0
33	136428.0
34	162524.0
35	195276.0
36	241247.0
37	307704.0
38	387230.0
39	277896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.01248492829718	22.581247673703203	14.077319241956921	23.3289481560427
2	19.959007668734436	24.16778771481255	33.56757951265573	22.30562510379728
3	20.36983112041655	25.00284668967122	32.18856652409965	22.438755665812586
4	14.798645650820768	17.209172956763172	35.58564620305606	32.40653518936
5	12.641237439122472	39.04852604484763	34.77084035629303	13.53939615973687
6	32.665010110811615	37.709119173456344	13.434980032666044	16.190890683066
7	26.44986518167731	32.75263864503873	21.293958851944684	19.50353732133928
8	27.396721423623877	34.51790128985422	18.68999166921331	19.395385617308595
9	24.859319521900158	14.144154564672515	21.111545678230005	39.884980235197325
10	16.47677483787371	27.827257919985744	33.512130948625014	22.183836293515533
11	33.998296036822886	22.395548992541897	22.94206840246476	20.664086568170454
12	21.056457169809878	27.21331809698008	30.595275440291754	21.134949292918293
13	31.609192039710532	20.79190630992957	25.79100842125066	21.807893229109236
14	20.739293183832245	22.727025189040447	27.24257261534044	29.29110901178687
15	23.15184580258421	29.622900256944686	24.812872348134167	22.412381592336935
16	22.351847242806652	28.082672368747353	26.155609733923402	23.409870654522592
17	22.18302616839171	26.88683767207845	26.783501711839392	24.146634447690445
18	23.034782722191444	25.69334333687838	28.88996205463934	22.381911886290837
19	23.0699331511752	24.988084409637068	29.909324495168278	22.032657944019455
20	23.827895218416483	24.76593009790362	29.696531629310147	21.70964305436975
21	23.048959911858386	25.451475980465183	29.96045239187192	21.539111715804506
22	21.872163155599385	25.84857231199359	29.66457669387037	22.61468783853666
23	22.733146134420462	26.027519950456345	30.347827221914425	20.89150669320877
24	22.60573145522326	26.053173912710818	28.207071582205874	23.13402304986005
25	22.687104023216385	26.053849016980674	29.249972658277073	22.00907430152587
26	22.44019588825494	26.77801086377791	29.201680199506814	21.580113048460333
27	21.70406219240562	26.763833674110966	28.800578249310604	22.73152588417281
28	22.014700170441326	26.513324983043628	29.103114976108056	22.368859870406986
29	22.339110275582065	26.448424959234956	28.532021770762494	22.68044299442049
30	22.16443829749505	26.54676514787709	29.90995459248681	21.378841962141053
31	22.667661020244577	26.44414929885921	29.757471041402344	21.13071863949387
32	22.248556289518916	26.243058240345224	28.897433208559065	22.6109522615768
33	22.027212102909292	26.37191314198478	29.3822030812659	22.218671673840024
34	21.535511159698615	26.836519900498633	29.732132127807137	21.89583681199561
35	21.1633486792035	28.53530727820912	28.872319329720476	21.4290247128669
36	23.01804013629905	26.523811602702036	28.69728729602287	21.76086096497604
37	22.116550901286704	27.128164945075767	28.672758507551492	22.082525646086037
38	22.035493381952843	25.898845076622084	29.884345637183664	22.181315904241412
39	21.502566071329717	25.97630203985005	30.474431775987803	22.046700112832426
40	20.73470247479723	26.478714637475758	30.090432467294576	22.69615042043244
41	21.84511397785388	25.41403019696392	30.198764199130558	22.542091626051644
42	20.81670514010889	27.018753046408015	29.631046515134262	22.53349529834883
43	21.153357136009657	26.596362808235728	29.121477812248102	23.12880224350651
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	238.0
2	345.0
3	667.0
4	989.0
5	989.0
6	1694.0
7	2399.0
8	2928.0
9	3457.0
10	5391.5
11	7326.0
12	7326.0
13	14743.0
14	22160.0
15	35857.5
16	49555.0
17	44869.5
18	40184.0
19	40184.0
20	44318.5
21	48453.0
22	39479.0
23	30505.0
24	34292.0
25	38079.0
26	38079.0
27	42061.0
28	46043.0
29	50365.5
30	54688.0
31	61248.0
32	67808.0
33	67808.0
34	76272.5
35	84737.0
36	92777.5
37	100818.0
38	107090.0
39	113362.0
40	113362.0
41	117347.5
42	121333.0
43	127673.0
44	134013.0
45	140286.0
46	146559.0
47	146559.0
48	179923.0
49	213287.0
50	203638.0
51	193989.0
52	180787.5
53	167586.0
54	167586.0
55	151370.5
56	135155.0
57	118310.5
58	101466.0
59	91104.5
60	80743.0
61	80743.0
62	72509.5
63	64276.0
64	56154.0
65	48032.0
66	41403.5
67	34775.0
68	34775.0
69	30070.0
70	25365.0
71	21337.5
72	17310.0
73	14281.5
74	11253.0
75	11253.0
76	9093.5
77	6934.0
78	5467.5
79	4001.0
80	3149.0
81	2297.0
82	2297.0
83	1739.5
84	1182.0
85	918.0
86	654.0
87	491.5
88	329.0
89	329.0
90	257.5
91	186.0
92	130.0
93	74.0
94	51.5
95	29.0
96	29.0
97	19.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2221879.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.144615651830385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8700815790537	43.50954018902809
2	10.492823419218386	11.152741354337717
3	3.1709718339055053	5.055602380670634
4	1.2955613549850007	2.7540844105616977
5	0.73648756773327	1.9570174359768013
6	0.43209063523150365	1.3777974441680103
7	0.31533061252303946	1.1730686944055229
8	0.24169099877727696	1.0275660189220313
9	0.15382916858351603	0.7357672836370424
>10	1.105746598104442	11.225169429008867
>50	0.10408878076088846	3.790443600676844
>100	0.07176588061159024	7.317299406803988
>500	0.0058721282617863356	2.1846849080202753
>1k	0.003233925709389573	3.3174610384913676
>5k	1.7020661628366172E-4	0.6574769068377507
>10k+	2.553099244254926E-4	2.7642794984533596
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24732	1.113111920136065	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21006	0.9454160195042125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15381	0.6922519183087829	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8973	0.40384737422694933	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5564	0.2504186771646881	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4941	0.22237934649006538	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4908	0.22089411709638554	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4575	0.2059068023056161	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4436	0.19965083607163125	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3545	0.1595496424422752	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2656	0.11953846271556642	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2581	0.11616294136629403	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2472	0.11125718367201813	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2247	0.10113061962420096	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.500695132363193E-5
2	0.0	0.0	0.0	0.0	4.500695132363193E-5
3	0.0	0.0	0.0	0.0	4.500695132363193E-5
4	0.0	0.0	0.0	0.0	4.500695132363193E-5
5	0.0	0.0	0.0	0.0	9.001390264726387E-5
6	0.0	0.0	0.0	0.0	9.001390264726387E-5
7	0.0	0.0	0.0	4.500695132363193E-5	9.001390264726387E-5
8	0.0	0.0	0.0	4.500695132363193E-5	9.001390264726387E-5
9	0.0	0.0	0.0	9.001390264726387E-5	9.001390264726387E-5
10	9.001390264726387E-5	0.0	0.0	9.001390264726387E-5	9.001390264726387E-5
11	9.001390264726387E-5	0.0	0.0	1.350208539708958E-4	9.001390264726387E-5
12	9.001390264726387E-5	0.0	0.0	2.700417079417916E-4	9.001390264726387E-5
13	9.001390264726387E-5	0.0	0.0	3.1504865926542357E-4	9.001390264726387E-5
14	9.001390264726387E-5	0.0	0.0	4.950764645599513E-4	9.001390264726387E-5
15	9.001390264726387E-5	0.0	0.0	5.850903672072152E-4	1.350208539708958E-4
16	9.001390264726387E-5	0.0	0.0	6.300973185308471E-4	1.350208539708958E-4
17	9.001390264726387E-5	0.0	0.0	0.0010351598804435346	1.350208539708958E-4
18	9.001390264726387E-5	0.0	0.0	0.001350208539708958	1.350208539708958E-4
19	1.350208539708958E-4	0.0	0.0	0.0019352989069161732	1.350208539708958E-4
20	1.350208539708958E-4	0.0	0.0	0.0030604726900069715	1.350208539708958E-4
21	1.350208539708958E-4	0.0	0.0	0.006210959282661207	1.350208539708958E-4
22	2.250347566181597E-4	0.0	0.0	0.01021657795046445	1.350208539708958E-4
23	2.250347566181597E-4	0.0	0.0	0.01210686990605699	1.350208539708958E-4
24	2.250347566181597E-4	0.0	0.0	0.015932460768565707	1.350208539708958E-4
25	2.250347566181597E-4	0.0	0.0	0.01836283614004183	1.350208539708958E-4
26	2.250347566181597E-4	0.0	0.0	0.023808677250201294	1.350208539708958E-4
27	2.250347566181597E-4	0.0	0.0	0.058959106233957836	1.350208539708958E-4
28	2.250347566181597E-4	0.0	0.0	0.1320503951835361	1.350208539708958E-4
29	2.250347566181597E-4	0.0	0.0	0.22440465929962883	1.350208539708958E-4
30	2.250347566181597E-4	0.0	0.0	0.35361961654977614	1.350208539708958E-4
31	2.250347566181597E-4	0.0	0.0	0.49359123516627146	1.8002780529452773E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6445	0.0	23.82467	1
TTCGTTA	170	5.456968E-12	17.411764	30
CGTTAAC	65	0.001580561	17.076923	10
GTATCAA	9555	0.0	16.012035	2
CTAATAC	555	0.0	15.999999	3
TAATACT	635	0.0	15.732285	4
GTATTAG	550	0.0	15.136363	1
GTATTGG	820	0.0	14.890244	1
ACGTTCG	175	2.240995E-9	14.8	22
TTAACGG	315	0.0	14.682539	35
TTAGACT	255	0.0	14.509804	4
ACGGGTA	195	6.730261E-10	14.23077	5
TTGGACC	1300	0.0	14.230769	4
CTCTAAT	445	0.0	14.134832	1
ATTGGAC	1105	0.0	14.063348	3
GTACACT	135	6.5731E-6	13.703704	4
TCTATAC	205	1.4260877E-9	13.536585	3
TGCGGTA	165	2.5980626E-7	13.454545	36
CGTTCGC	220	2.8558134E-10	13.454545	23
TACCCCG	400	0.0	13.4125	5
>>END_MODULE