FastQCFastQC Report
Wed 25 May 2016
SRR2088464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844648
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT349681.2292557813831448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT295431.0385467727465754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238670.8390141767979729No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125060.43963260129197007No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG78320.2753240471228778No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG69480.2442481459920524No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA66500.2337723331674077No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA60180.21155517308292623No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA59040.20754764737148496No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG53850.18930285926413393No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37430.13158042752565519No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37170.1306664304335721No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC35190.1237059910400162No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA34530.12138584457549756No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32540.11439025144763078No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.11407386783883278No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.10117244734673674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92000.024.9751
GTATTGG10300.017.6019421
GTATCAA144900.015.869912
CTAATAC6250.015.6883
GTATTAG6300.015.2698421
TTGGACC15500.015.0387094
ATTGGAC12750.014.9450983
TAATACT7500.014.7999994
TGGACCC15650.014.6581475
TATAGTG2800.014.5357145
CCGTGCG1551.212029E-714.3225819
GGACCCT16900.014.230776
TACACAG4550.014.230775
GTAAGAT3400.014.1470593
TATACTG3050.013.950825
ACCGTTA4450.013.7191028
GCACTAG1356.5746553E-613.7037031
GTCTTAT2304.0017767E-1113.6739131
TAGTACT3850.013.4545454
CGTTCGC3200.013.29687623
Produced by FastQC (version 0.11.5)