FastQCFastQC Report
Wed 25 May 2016
SRR2088463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137060
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT191061.680298313193675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158361.3927145445271139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118641.0433926090091992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.5951313035371923No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG40820.3589960072467592No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG40130.35292772588957483No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA38050.3346349357113961No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA35770.31458322340070005No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34400.3025346067929573No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG29750.26163966721193255No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20740.18240022514203297No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA19590.1722864228800591No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19250.16929625525477987No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC18910.16630608762950064No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17470.15364184827537686No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.15355390216875098No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA15690.13798744129597385No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14280.12558704026172762No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.12338838759608112No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC13560.11925492058466572No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG13170.11582502242625718No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA12750.11213128594797109No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC12640.11116387877508664No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG12350.10861344168293668No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT12210.10738219619017467No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT11610.10210542979262308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG405.9400765E-527.755
GGTATCA51950.024.0375371
ATTTCGG802.7257556E-823.12529
ACGTTTA555.143048E-420.18181826
TGTACTA609.2367333E-418.55
CGTAAAC1054.797148E-717.61904732
GACGAGT1054.797148E-717.61904725
GTGTAAA953.6063193E-617.5263161
TATAGCC852.7230748E-517.4117643
GCCGTTT2850.016.87719326
GTATTGG5600.016.8482151
CGTTTAC2650.016.75471528
CCGTTTA2650.016.75471527
CGCCGTT2800.016.51785925
TGCGACG904.4458902E-516.44444522
AGACCGT904.4458902E-516.4444456
CTCGAGT904.4458902E-516.44444530
GGTCGTA1251.6575541E-716.27999929
ACTATTC803.3825263E-416.18753
TCACGTT803.3825263E-416.187524
Produced by FastQC (version 0.11.5)