##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137060
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16692171037588	31.0	31.0	33.0	30.0	34.0
2	31.30550454681371	31.0	31.0	34.0	30.0	34.0
3	31.41991451638436	31.0	31.0	34.0	28.0	34.0
4	35.15368142402336	35.0	35.0	37.0	33.0	37.0
5	35.01926459465639	35.0	35.0	37.0	32.0	37.0
6	35.12386593495506	36.0	35.0	37.0	32.0	37.0
7	34.934510052239986	36.0	35.0	37.0	32.0	37.0
8	35.01201431762616	36.0	35.0	37.0	32.0	37.0
9	36.48489877403127	38.0	35.0	39.0	32.0	39.0
10	36.31987318171424	38.0	35.0	39.0	32.0	39.0
11	36.36335197790794	38.0	35.0	39.0	32.0	39.0
12	36.19735546057376	38.0	35.0	39.0	32.0	39.0
13	36.23978242133221	38.0	35.0	39.0	32.0	39.0
14	37.05815260408422	39.0	36.0	40.0	31.0	41.0
15	37.11223682127592	39.0	36.0	40.0	31.0	41.0
16	36.94727279123353	38.0	36.0	40.0	31.0	41.0
17	37.072223101683285	39.0	36.0	40.0	31.0	41.0
18	36.953401755404286	39.0	36.0	40.0	31.0	41.0
19	37.04736601410656	39.0	36.0	40.0	31.0	41.0
20	36.97039206374334	39.0	36.0	40.0	31.0	41.0
21	36.80565757303924	39.0	36.0	40.0	30.0	41.0
22	36.70531546268447	39.0	35.0	40.0	30.0	41.0
23	36.48043287073681	38.0	35.0	40.0	30.0	41.0
24	36.67807151777391	39.0	35.0	40.0	30.0	41.0
25	36.628412748667614	39.0	35.0	40.0	30.0	41.0
26	36.39902467767752	38.0	35.0	40.0	30.0	41.0
27	36.323865055494	38.0	35.0	40.0	30.0	41.0
28	36.10163755650537	38.0	35.0	40.0	29.0	41.0
29	35.90324609079556	38.0	34.0	40.0	29.0	41.0
30	35.68587057850949	38.0	34.0	40.0	27.0	41.0
31	35.3419617258544	38.0	34.0	40.0	27.0	41.0
32	35.19408210648515	38.0	34.0	40.0	26.0	41.0
33	34.965705415721246	38.0	33.0	40.0	25.0	41.0
34	34.81385942694317	37.0	33.0	40.0	25.0	41.0
35	34.541158777900904	37.0	33.0	40.0	24.0	41.0
36	34.35857474539602	37.0	33.0	40.0	24.0	41.0
37	34.16909046136527	37.0	33.0	40.0	23.0	41.0
38	34.020064024765624	37.0	33.0	40.0	22.0	41.0
39	33.858762950064204	37.0	33.0	40.0	21.0	41.0
40	33.679118076442755	37.0	32.0	40.0	21.0	41.0
41	33.40467785341143	37.0	32.0	40.0	18.0	41.0
42	33.098047596432906	37.0	31.0	40.0	16.0	41.0
43	32.74091252880235	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	1.0
15	3.0
16	31.0
17	63.0
18	144.0
19	330.0
20	741.0
21	1505.0
22	2680.0
23	4573.0
24	7105.0
25	10454.0
26	14651.0
27	19917.0
28	26120.0
29	33226.0
30	40491.0
31	48823.0
32	58653.0
33	70687.0
34	83426.0
35	99198.0
36	124258.0
37	159848.0
38	188462.0
39	141667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.59539514185707	22.328549065132886	14.932369444004715	22.14368634900533
2	19.71197650080031	24.736689356762177	33.8729706435896	21.678363498847904
3	20.49258614321144	24.70731535714914	33.475454241640726	21.3246442579987
4	14.431076636237314	16.98353648883964	35.27166552336728	33.31372135155576
5	12.094436529294848	39.68462526163967	34.34436177510422	13.876576433961269
6	32.19601428244771	37.34130125059363	12.691942377710937	17.770742089247708
7	25.28468154714791	33.86927690711132	20.746486553040295	20.099554992700472
8	27.99368546954426	34.16653474750673	18.18048300001759	19.65929678293142
9	24.253425500853076	14.330202451937451	21.99611278208714	39.42025926512233
10	17.03041176367122	28.1046734561061	32.37683147767048	22.488083302552194
11	33.00080910418096	22.497581482067787	23.299210243962502	21.202399169788755
12	21.22245088209945	28.017430918333247	30.473589784180255	20.28652841538705
13	32.84408914217368	20.71711255342726	26.150598912986123	20.28819939141294
14	20.25442808646861	23.73551087893339	27.895537614549802	28.114523420048194
15	22.470669973440277	30.174748913865585	25.71157194870983	21.64300916398431
16	21.289026084815223	28.44115526005664	26.988549416917312	23.281269238210825
17	21.72180887552108	26.827695987898615	27.04888044606265	24.40161469051765
18	23.222433292878126	25.0051008741843	29.926389108754154	21.84607672418342
19	22.685962042460382	24.49659648567358	31.51232124953828	21.30512022232776
20	23.78053928552583	24.014036198617486	31.22526515751148	20.980159358345208
21	22.55958348723902	24.895168240901974	32.10393470881044	20.441313563048563
22	20.863103090426186	25.43858723374316	31.470634794997626	22.227674880833025
23	22.134627900022867	25.75959052292755	32.5068158232635	19.59896575378608
24	21.896997519919793	25.73179955323378	29.727015285033332	22.644187641813097
25	21.699206726118234	26.07056795595659	30.936538089458782	21.293687228466396
26	21.59481469755334	26.905000615622747	30.874008407647796	20.62617627917612
27	20.29224491231773	26.949853130001934	30.569890770935572	22.188011186744763
28	21.003816861027563	26.60651152973458	30.496543718009605	21.893127891228257
29	21.23836912739873	26.41259036462456	29.662550788876572	22.686489719100138
30	21.176103283907622	26.65321091235291	31.939651381633333	20.231034422106134
31	21.988285578597434	26.41320598737094	31.600091463950893	19.998416970080733
32	21.581183051026333	26.27064534853042	30.271841415580532	21.876330184862717
33	21.039874764744166	26.409688143105903	30.88236328777725	21.668073804372682
34	20.095245633475805	27.22697131198002	31.513816333350924	21.163966721193255
35	19.622535310361812	29.584454646192814	30.33753715723005	20.45547288621533
36	22.38800063321197	26.671327810317834	30.197439009375053	20.74323254709514
37	21.189822876541257	27.809438376163087	29.86579424128894	21.13494450600672
38	21.095104919705204	25.981214711624716	31.582941973158846	21.34073839551123
39	20.50560216699207	25.70717464337854	32.36363956167661	21.42358362795279
40	19.137072801787063	26.610820888959246	31.79366084463441	22.45844546461928
41	20.910330149684274	25.480537526603698	31.759801593583454	21.849330730128578
42	19.542240515012402	27.613494450600673	31.181116211985294	21.66314882240163
43	19.777056619703444	27.539443828821696	30.22373489525619	22.45976465621867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	123.0
2	183.0
3	432.0
4	681.0
5	681.0
6	1219.0
7	1757.0
8	2066.5
9	2376.0
10	3892.0
11	5408.0
12	5408.0
13	10907.5
14	16407.0
15	27061.0
16	37715.0
17	33183.0
18	28651.0
19	28651.0
20	30628.0
21	32605.0
22	24916.5
23	17228.0
24	18553.0
25	19878.0
26	19878.0
27	21270.0
28	22662.0
29	24154.5
30	25647.0
31	28162.5
32	30678.0
33	30678.0
34	33644.0
35	36610.0
36	40919.5
37	45229.0
38	47939.0
39	50649.0
40	50649.0
41	52889.5
42	55130.0
43	59055.0
44	62980.0
45	68653.5
46	74327.0
47	74327.0
48	102135.0
49	129943.0
50	121067.5
51	112192.0
52	102037.0
53	91882.0
54	91882.0
55	79576.5
56	67271.0
57	56918.5
58	46566.0
59	40957.5
60	35349.0
61	35349.0
62	31071.5
63	26794.0
64	23299.5
65	19805.0
66	16905.5
67	14006.0
68	14006.0
69	12081.5
70	10157.0
71	8384.5
72	6612.0
73	5370.5
74	4129.0
75	4129.0
76	3311.5
77	2494.0
78	1982.5
79	1471.0
80	1100.5
81	730.0
82	730.0
83	566.5
84	403.0
85	297.5
86	192.0
87	143.0
88	94.0
89	94.0
90	81.5
91	69.0
92	45.5
93	22.0
94	16.0
95	10.0
96	10.0
97	6.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1137060.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.406684948408966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.64511965204049	45.20615237676907
2	8.91047212249433	9.517575547752713
3	2.464522634098916	3.9486595160263143
4	1.0405039877642168	2.2227947464834683
5	0.5958129906982302	1.5910198341194848
6	0.40529812611656246	1.2987377599012662
7	0.26908654875173404	1.0059714373126984
8	0.2020766589942653	0.863379556986704
9	0.17375563270821587	0.8351741100653235
>10	1.1033918012741437	11.411268774539337
>50	0.1133250285445139	4.213769384697059
>100	0.0652386917076084	6.287493303715336
>500	0.006771610392471306	2.5871153362513346
>1k	0.003963869498031984	4.285367350190883
>5k	1.65161229084666E-4	0.5968976979342919
>10k+	4.95483687253998E-4	4.128623267254687
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19106	1.680298313193675	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15836	1.3927145445271139	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11864	1.0433926090091992	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6767	0.5951313035371923	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4082	0.3589960072467592	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4013	0.35292772588957483	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3805	0.3346349357113961	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3577	0.31458322340070005	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3440	0.3025346067929573	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2975	0.26163966721193255	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2074	0.18240022514203297	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1959	0.1722864228800591	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1925	0.16929625525477987	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1891	0.16630608762950064	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1747	0.15364184827537686	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1746	0.15355390216875098	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1569	0.13798744129597385	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1428	0.12558704026172762	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1403	0.12338838759608112	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1356	0.11925492058466572	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1317	0.11582502242625718	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1275	0.11213128594797109	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1264	0.11116387877508664	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1235	0.10861344168293668	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1221	0.10738219619017467	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1161	0.10210542979262308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.794610662585967E-5	0.0
4	0.0	0.0	0.0	8.794610662585967E-5	0.0
5	0.0	0.0	0.0	8.794610662585967E-5	0.0
6	0.0	0.0	0.0	8.794610662585967E-5	0.0
7	0.0	0.0	0.0	8.794610662585967E-5	0.0
8	0.0	0.0	0.0	8.794610662585967E-5	0.0
9	8.794610662585967E-5	0.0	0.0	2.6383831987757903E-4	0.0
10	8.794610662585967E-5	0.0	0.0	3.517844265034387E-4	0.0
11	1.7589221325171935E-4	0.0	0.0	5.276766397551581E-4	8.794610662585967E-5
12	1.7589221325171935E-4	0.0	0.0	5.276766397551581E-4	8.794610662585967E-5
13	1.7589221325171935E-4	0.0	0.0	7.035688530068774E-4	8.794610662585967E-5
14	1.7589221325171935E-4	0.0	0.0	9.674071728844564E-4	8.794610662585967E-5
15	1.7589221325171935E-4	8.794610662585967E-5	0.0	0.0012312454927620353	8.794610662585967E-5
16	1.7589221325171935E-4	8.794610662585967E-5	0.0	0.0021986526656464916	1.7589221325171935E-4
17	1.7589221325171935E-4	8.794610662585967E-5	0.0	0.0028142754120275095	1.7589221325171935E-4
18	1.7589221325171935E-4	8.794610662585967E-5	0.0	0.0033419520517826676	1.7589221325171935E-4
19	4.3973053312929835E-4	8.794610662585967E-5	0.0	0.0044852514379188435	1.7589221325171935E-4
20	4.3973053312929835E-4	8.794610662585967E-5	0.0	0.008266934022830809	1.7589221325171935E-4
21	4.3973053312929835E-4	8.794610662585967E-5	0.0	0.012400401034246214	3.517844265034387E-4
22	4.3973053312929835E-4	8.794610662585967E-5	0.0	0.022514203296220076	3.517844265034387E-4
23	4.3973053312929835E-4	8.794610662585967E-5	0.0	0.028230700226900954	3.517844265034387E-4
24	4.3973053312929835E-4	8.794610662585967E-5	0.0	0.03570611929009903	3.517844265034387E-4
25	4.3973053312929835E-4	1.7589221325171935E-4	0.0	0.03913601744850755	3.517844265034387E-4
26	4.3973053312929835E-4	1.7589221325171935E-4	0.0	0.04898598139060384	3.517844265034387E-4
27	4.3973053312929835E-4	1.7589221325171935E-4	0.0	0.10571122016428333	3.517844265034387E-4
28	4.3973053312929835E-4	1.7589221325171935E-4	0.0	0.2163474222996148	3.517844265034387E-4
29	4.3973053312929835E-4	1.7589221325171935E-4	0.0	0.36954954004186236	3.517844265034387E-4
30	4.3973053312929835E-4	1.7589221325171935E-4	0.0	0.583434471355953	3.517844265034387E-4
31	4.3973053312929835E-4	1.7589221325171935E-4	0.0	0.8047948217332419	3.517844265034387E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	5.9400765E-5	27.75	5
GGTATCA	5195	0.0	24.037537	1
ATTTCGG	80	2.7257556E-8	23.125	29
ACGTTTA	55	5.143048E-4	20.181818	26
TGTACTA	60	9.2367333E-4	18.5	5
CGTAAAC	105	4.797148E-7	17.619047	32
GACGAGT	105	4.797148E-7	17.619047	25
GTGTAAA	95	3.6063193E-6	17.526316	1
TATAGCC	85	2.7230748E-5	17.411764	3
GCCGTTT	285	0.0	16.877193	26
GTATTGG	560	0.0	16.848215	1
CGTTTAC	265	0.0	16.754715	28
CCGTTTA	265	0.0	16.754715	27
CGCCGTT	280	0.0	16.517859	25
TGCGACG	90	4.4458902E-5	16.444445	22
AGACCGT	90	4.4458902E-5	16.444445	6
CTCGAGT	90	4.4458902E-5	16.444445	30
GGTCGTA	125	1.6575541E-7	16.279999	29
ACTATTC	80	3.3825263E-4	16.1875	3
TCACGTT	80	3.3825263E-4	16.1875	24
>>END_MODULE