FastQCFastQC Report
Wed 25 May 2016
SRR2088462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550522
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT339591.3314529339484231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT287301.1264360785752876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT226170.886759651553682No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125350.49146802105608184No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76960.3017421531749187No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG71020.27845280299483793No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA69900.2740615450484254No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA64220.2517915940344761No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA60150.2358340763184948No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG57730.22634582254142482No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37920.14867544761425308No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA36340.14248063729699253No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35820.14044183896472956No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34630.13577612739666625No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32430.1271504421447845No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.1247979825306349No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28590.1120947006142272No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27920.10946778737842686No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG25510.10001874126159274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97950.023.0801431
GTATTGG11300.018.1725671
ATTGGAC12350.016.3279343
TATACTG3300.016.2575765
TTGGACC15250.015.649184
TATTGGA13300.015.0225572
TTCGTTA2600.014.94230730
ACCGTTA5200.014.9423078
GTATCAA152050.014.8316342
CGAACTA2750.014.824
GCGCGTA750.004106231514.79999911
CTAATAC4300.014.6279073
CCGTTTA5100.014.50980427
TGGACCC15750.014.44761855
ACGTTCG2750.014.12727422
TACACCG2650.013.9622645
TTGCGAC1203.3034605E-513.87500115
GGTCTAG1203.3034605E-513.8750011
GTATACT2151.9826984E-1013.7674424
TAGTACC2304.0017767E-1113.6739134
Produced by FastQC (version 0.11.5)