##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2550522
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406074129139053	31.0	31.0	34.0	30.0	34.0
2	31.536587020225664	31.0	31.0	34.0	30.0	34.0
3	31.675198645610585	31.0	31.0	34.0	30.0	34.0
4	35.3802790173933	37.0	35.0	37.0	33.0	37.0
5	35.25443811110039	37.0	35.0	37.0	33.0	37.0
6	35.3327957963115	37.0	35.0	37.0	33.0	37.0
7	35.164789403894574	37.0	35.0	37.0	32.0	37.0
8	35.21848272628113	37.0	35.0	37.0	32.0	37.0
9	36.75484626284345	39.0	35.0	39.0	32.0	39.0
10	36.611918266143164	39.0	35.0	39.0	32.0	39.0
11	36.63896096563762	39.0	35.0	39.0	32.0	39.0
12	36.48805969915178	38.0	35.0	39.0	32.0	39.0
13	36.521860230964485	38.0	35.0	39.0	32.0	39.0
14	37.45000003920766	39.0	36.0	41.0	32.0	41.0
15	37.48363158600475	39.0	36.0	41.0	32.0	41.0
16	37.347983667657054	39.0	36.0	41.0	32.0	41.0
17	37.433753168959136	39.0	36.0	41.0	32.0	41.0
18	37.30434593389118	39.0	36.0	41.0	32.0	41.0
19	37.38571751194461	39.0	36.0	41.0	32.0	41.0
20	37.29481886453048	39.0	36.0	41.0	31.0	41.0
21	37.14509657238793	39.0	36.0	40.0	31.0	41.0
22	37.041224502278354	39.0	36.0	40.0	31.0	41.0
23	36.83346193445891	39.0	35.0	40.0	30.0	41.0
24	37.037039868701385	39.0	36.0	40.0	31.0	41.0
25	37.00040462305363	39.0	36.0	41.0	31.0	41.0
26	36.8001091541261	39.0	36.0	40.0	30.0	41.0
27	36.729231898411385	39.0	35.0	40.0	30.0	41.0
28	36.528997985510415	39.0	35.0	40.0	30.0	41.0
29	36.355630337632846	38.0	35.0	40.0	30.0	41.0
30	36.160960775872546	38.0	35.0	40.0	30.0	41.0
31	35.87178467780321	38.0	35.0	40.0	29.0	41.0
32	35.73008740955773	38.0	34.0	40.0	28.0	41.0
33	35.511053423573685	38.0	34.0	40.0	27.0	41.0
34	35.388768259987565	38.0	34.0	40.0	27.0	41.0
35	35.13162207579468	38.0	34.0	40.0	26.0	41.0
36	34.96177331542327	38.0	33.0	40.0	25.0	41.0
37	34.794484815265264	38.0	33.0	40.0	24.0	41.0
38	34.6880862035301	38.0	33.0	40.0	24.0	41.0
39	34.56676398007937	38.0	33.0	40.0	23.0	41.0
40	34.411964295936286	38.0	33.0	40.0	23.0	41.0
41	34.17899433919801	38.0	33.0	40.0	22.0	41.0
42	33.919134984916816	37.0	33.0	40.0	20.0	41.0
43	33.57091254260892	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	11.0
15	26.0
16	58.0
17	92.0
18	282.0
19	602.0
20	1245.0
21	2555.0
22	4588.0
23	7951.0
24	12555.0
25	18696.0
26	27272.0
27	36439.0
28	49301.0
29	63654.0
30	79431.0
31	97673.0
32	119050.0
33	144558.0
34	174879.0
35	211681.0
36	268081.0
37	353265.0
38	442710.0
39	433860.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44030124029513	21.266430950213326	14.026462034046364	23.266805775445185
2	19.319417750562433	23.82128050650024	34.371081684455184	22.48822005848215
3	19.989476663992704	24.55391484566689	32.75925477216037	22.697353718180043
4	14.122560009284374	16.42800963881119	35.62286465280441	33.82656569910002
5	12.63423722673241	38.69023674369404	34.55237790538565	14.123148124187912
6	32.55106209630813	37.01140394005619	13.25807030874464	17.179463654891038
7	26.12453450705385	33.04703899829133	21.03251804924639	19.79590844540843
8	27.78866443810326	33.88890587887499	18.525305800146008	19.79712388287574
9	24.777241678370153	14.294877676020832	21.48360218025957	39.44427846534945
10	16.792327217722487	27.556633504827637	32.88518977683784	22.76584950061203
11	33.40861204098612	22.3858488576064	23.18831203965306	21.01722706175442
12	21.388405981206983	27.229955279742736	30.51269504830776	20.868943690742523
13	32.19748741630145	20.768807326500223	25.82138087811044	21.21232437908789
14	20.691176159233287	23.181685945073205	27.434619266173748	28.69251862951976
15	22.94067645760358	29.51137061354499	25.219425670509803	22.328527258341627
16	21.977030584327444	28.14815947480555	26.386324054448462	23.488485886418545
17	22.096339494425063	26.710257743316856	26.637370702938455	24.55603205931962
18	23.382233127179454	25.22279752929008	29.131840462462193	22.263128881068265
19	23.150476647525487	24.619509261241422	30.391739416480235	21.838274674752856
20	24.04805761330426	24.17238510391206	30.156493455065274	21.623063827718404
21	22.851282992265897	25.061183553798006	30.750881584240403	21.336651869695693
22	21.540022003338922	25.498937080331007	30.34708189147163	22.613959024858442
23	22.6002363437759	25.697876748367587	31.100653121204207	20.6012337866523
24	22.41611717130846	25.703483443781312	28.813356638366578	23.06704274654365
25	22.371381230979384	25.95931342682008	29.87466879329016	21.79463654891038
26	22.145819561642675	26.633253898613695	29.820013314921418	21.40091322482221
27	21.040359581293554	26.706415392613746	29.5550479470477	22.698177079044996
28	21.676307830318656	26.379423506246958	29.430955702401313	22.513312961033076
29	21.84141128757172	26.279287142004655	28.979126625843648	22.900174944579973
30	21.748763586434464	26.572717271209576	30.684032523538317	20.994486618817636
31	22.477516367237765	26.20173439005819	30.476663208551035	20.84408603415301
32	22.217099087951407	26.13845322643757	29.225938847028175	22.418508838582845
33	21.69516671489209	26.243568963529817	29.89599776045845	22.165266561119644
34	20.89658509120878	26.746367998394057	30.43945513898724	21.91759177140993
35	20.47894509437676	28.796771798086823	29.540149036158088	21.18413407137833
36	22.666536497234684	26.37558115554385	29.386023723771054	21.571858623450417
37	21.727434619266173	27.14460020340934	29.28263312372918	21.845332053595303
38	21.56468362162726	25.693955982344008	30.637571446158862	22.10378894986987
39	21.13641834887133	25.4511037348433	31.37322477516367	22.0392531411217
40	20.04664143261654	26.062468780900538	31.0381561107883	22.852733675694623
41	21.30830473134519	25.023622615292084	31.110062959660805	22.558009693701916
42	20.08392791750081	26.8922989097918	30.540218825793307	22.483554346914083
43	20.39821652195119	26.772597923091823	29.801468091629868	23.027717463327114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	337.5
2	504.0
3	1083.5
4	1663.0
5	1663.0
6	2849.5
7	4036.0
8	4524.5
9	5013.0
10	7893.5
11	10774.0
12	10774.0
13	21074.0
14	31374.0
15	49566.0
16	67758.0
17	61481.0
18	55204.0
19	55204.0
20	58587.0
21	61970.0
22	48749.0
23	35528.0
24	39216.0
25	42904.0
26	42904.0
27	46621.0
28	50338.0
29	54339.0
30	58340.0
31	63801.0
32	69262.0
33	69262.0
34	77110.5
35	84959.0
36	93575.0
37	102191.0
38	109377.5
39	116564.0
40	116564.0
41	121320.5
42	126077.0
43	134488.0
44	142899.0
45	155275.5
46	167652.0
47	167652.0
48	219591.0
49	271530.0
50	256064.0
51	240598.0
52	221828.5
53	203059.0
54	203059.0
55	180614.0
56	158169.0
57	137042.5
58	115916.0
59	103033.0
60	90150.0
61	90150.0
62	79967.5
63	69785.0
64	61002.0
65	52219.0
66	45008.5
67	37798.0
68	37798.0
69	32741.5
70	27685.0
71	23535.5
72	19386.0
73	15896.0
74	12406.0
75	12406.0
76	9862.0
77	7318.0
78	5761.5
79	4205.0
80	3340.5
81	2476.0
82	2476.0
83	1865.5
84	1255.0
85	978.0
86	701.0
87	512.0
88	323.0
89	323.0
90	266.0
91	209.0
92	153.0
93	97.0
94	64.5
95	32.0
96	32.0
97	24.5
98	17.0
99	12.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2550522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.99256894060688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5311449268134	38.96435100752302
2	11.437537872509504	11.20708725459448
3	3.7454183242362062	5.504929963846639
4	1.5990939686457748	3.1337488600554715
5	0.8591264147206642	2.1045405050949277
6	0.5521547262813101	1.6230887095931406
7	0.38132217672947527	1.307736712240077
8	0.25146693043828605	0.985600874062442
9	0.19816058142277282	0.8737556351999347
>10	1.2317013172706048	11.588831301759777
>50	0.12331289022646476	4.233424538874308
>100	0.07916701903112087	7.392995224632566
>500	0.00523670820798404	1.7488299265463976
>1k	0.004350496049709543	3.890472623738099
>5k	4.833884499677269E-4	1.5787505225359302
>10k+	3.222589666451513E-4	3.861856339702934
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33959	1.3314529339484231	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28730	1.1264360785752876	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22617	0.886759651553682	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12535	0.49146802105608184	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7696	0.3017421531749187	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7102	0.27845280299483793	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6990	0.2740615450484254	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6422	0.2517915940344761	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6015	0.2358340763184948	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5773	0.22634582254142482	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3792	0.14867544761425308	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3634	0.14248063729699253	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3582	0.14044183896472956	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3463	0.13577612739666625	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3243	0.1271504421447845	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3183	0.1247979825306349	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2859	0.1120947006142272	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2792	0.10946778737842686	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2551	0.10001874126159274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.9207660235826235E-5	0.0
3	0.0	0.0	0.0	3.9207660235826235E-5	0.0
4	0.0	0.0	0.0	3.9207660235826235E-5	0.0
5	0.0	0.0	0.0	3.9207660235826235E-5	0.0
6	0.0	0.0	0.0	3.9207660235826235E-5	0.0
7	0.0	0.0	0.0	7.841532047165247E-5	0.0
8	0.0	0.0	0.0	7.841532047165247E-5	0.0
9	0.0	0.0	0.0	7.841532047165247E-5	0.0
10	0.0	0.0	0.0	1.9603830117913116E-4	0.0
11	0.0	0.0	0.0	3.528689421224361E-4	0.0
12	0.0	0.0	0.0	5.489072433015673E-4	0.0
13	0.0	0.0	0.0	5.881149035373935E-4	0.0
14	3.9207660235826235E-5	0.0	0.0	6.66530224009046E-4	0.0
15	3.9207660235826235E-5	0.0	0.0	9.017761854240034E-4	0.0
16	7.841532047165247E-5	0.0	0.0	0.0013722681082539183	0.0
17	7.841532047165247E-5	0.0	0.0	0.0021956289732062693	0.0
18	7.841532047165247E-5	0.0	0.0	0.002666120896036184	0.0
19	1.5683064094330494E-4	0.0	0.0	0.0036855200621676663	0.0
20	1.5683064094330494E-4	0.0	0.0	0.00666530224009046	0.0
21	1.5683064094330494E-4	0.0	0.0	0.011174183167210477	0.0
22	1.5683064094330494E-4	0.0	0.0	0.01736899348447102	0.0
23	1.5683064094330494E-4	0.0	0.0	0.021015305886402862	0.0
24	1.5683064094330494E-4	0.0	0.0	0.02666120896036184	0.0
25	1.5683064094330494E-4	0.0	0.0	0.030778013285123595	0.0
26	1.5683064094330494E-4	0.0	0.0	0.039442906197241195	0.0
27	1.5683064094330494E-4	0.0	0.0	0.09707816674390576	0.0
28	1.5683064094330494E-4	0.0	0.0	0.21187819591440496	0.0
29	1.5683064094330494E-4	0.0	0.0	0.3647880708341273	0.0
30	1.5683064094330494E-4	0.0	0.0	0.5725886700840064	0.0
31	1.5683064094330494E-4	0.0	0.0	0.7896030694893046	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9795	0.0	23.080143	1
GTATTGG	1130	0.0	18.172567	1
ATTGGAC	1235	0.0	16.327934	3
TATACTG	330	0.0	16.257576	5
TTGGACC	1525	0.0	15.64918	4
TATTGGA	1330	0.0	15.022557	2
TTCGTTA	260	0.0	14.942307	30
ACCGTTA	520	0.0	14.942307	8
GTATCAA	15205	0.0	14.831634	2
CGAACTA	275	0.0	14.8	24
GCGCGTA	75	0.0041062315	14.799999	11
CTAATAC	430	0.0	14.627907	3
CCGTTTA	510	0.0	14.509804	27
TGGACCC	1575	0.0	14.4476185	5
ACGTTCG	275	0.0	14.127274	22
TACACCG	265	0.0	13.962264	5
TTGCGAC	120	3.3034605E-5	13.875001	15
GGTCTAG	120	3.3034605E-5	13.875001	1
GTATACT	215	1.9826984E-10	13.767442	4
TAGTACC	230	4.0017767E-11	13.673913	4
>>END_MODULE