FastQCFastQC Report
Wed 25 May 2016
SRR2088461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3968784
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360530.9084142649234628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT310040.7811964571516111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT248860.6270434470608629No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137080.34539546621836814No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG84890.21389423057541052No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA77790.1960046200549085No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG76290.19222512487452076No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68040.17143790138238815No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA66270.1669780970695306No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG60240.15178452644437188No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42630.10741325302661975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98650.023.7040041
TATACCG2600.016.3653855
GTATTGG13150.015.7566541
GTACGTA1353.9789848E-715.07407513
AGTTCCG5050.015.01980132
TTAACGG7900.014.98734235
TAATACT13600.014.8272054
GTATCAA159450.014.6306052
CTAATAC13250.014.5207553
CTCTAAT12150.014.465021
ATTGGAC16300.014.3006133
ACCGTTA6800.014.14705858
TAACGGC8300.014.04216936
TTGGACC20850.013.8417274
TCTAATA13400.013.6679112
GTATTAG13100.013.5572511
CCGTTTA7250.013.26896627
TGGACCC19250.013.0701295
GGACCGT8700.012.9712646
GGTAAAC11600.012.91810335
Produced by FastQC (version 0.11.5)