FastQCFastQC Report
Wed 25 May 2016
SRR2088460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2892648
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331351.1454902221079093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT278940.9643067528437611No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224320.7754832250588388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125420.43358196365406365No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG74750.25841374408500445No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG69280.23950373498607502No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA68470.23670353254180942No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA60600.2094966273117227No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA59480.2056247424505159No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55810.1929374054499545No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37450.1294661500466009No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37260.1288093124362176No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35970.12434973076572053No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34510.11930245228593316No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33240.11491201141652907No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32400.11200809777062401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96900.022.8911271
GTATTGG10950.020.2739731
GTATTAG9100.016.2637351
ATTGGAC14650.016.1638223
TAATACT9200.015.885874
TTAACGG5850.015.81196535
TGGACCC16450.015.5197575
AATACTG9650.015.1450785
TTGGACC18350.015.0217994
AGTACCG3250.014.85
GTATCAA154300.014.3875572
GGACCCT17400.014.2471276
ATACTGG8200.014.2134156
TAACGGC6400.014.164062536
ACCGTTA5100.014.14705858
GGTAAAC7500.014.06000135
CTAATAC9000.013.97777753
GTGACAC7950.013.96226324
ATTAGAG8750.013.9542863
TATTAGA9750.013.6615382
Produced by FastQC (version 0.11.5)