##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088460_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2892648
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.404613350812127	31.0	31.0	34.0	30.0	34.0
2	31.535373470951185	31.0	31.0	34.0	30.0	34.0
3	31.677072011527155	31.0	31.0	34.0	30.0	34.0
4	35.37899668400718	37.0	35.0	37.0	33.0	37.0
5	35.24948904947992	37.0	35.0	37.0	33.0	37.0
6	35.33048196669626	37.0	35.0	37.0	33.0	37.0
7	35.16870390037087	37.0	35.0	37.0	32.0	37.0
8	35.21537290399662	37.0	35.0	37.0	32.0	37.0
9	36.756863261620495	39.0	35.0	39.0	32.0	39.0
10	36.604813306008886	39.0	35.0	39.0	32.0	39.0
11	36.64005955788606	39.0	35.0	39.0	32.0	39.0
12	36.49365322016367	38.0	35.0	39.0	32.0	39.0
13	36.51866455925505	38.0	35.0	39.0	32.0	39.0
14	37.47414825447134	39.0	36.0	41.0	32.0	41.0
15	37.497268938356825	39.0	36.0	41.0	32.0	41.0
16	37.36274825004632	39.0	36.0	41.0	32.0	41.0
17	37.43154438424585	39.0	36.0	41.0	32.0	41.0
18	37.307818994914	39.0	36.0	41.0	32.0	41.0
19	37.393655916654915	39.0	36.0	41.0	32.0	41.0
20	37.301144141976486	39.0	36.0	41.0	31.0	41.0
21	37.141524651461225	39.0	36.0	40.0	31.0	41.0
22	37.04538713317348	39.0	36.0	40.0	31.0	41.0
23	36.857552664548194	39.0	36.0	40.0	30.0	41.0
24	37.06502519490792	39.0	36.0	40.0	31.0	41.0
25	37.01874752821636	39.0	36.0	41.0	31.0	41.0
26	36.8369372975903	39.0	36.0	40.0	30.0	41.0
27	36.771938721890805	39.0	35.0	40.0	30.0	41.0
28	36.58324414169992	39.0	35.0	40.0	30.0	41.0
29	36.41651766824031	39.0	35.0	40.0	30.0	41.0
30	36.23548561733055	38.0	35.0	40.0	30.0	41.0
31	35.98246416432279	38.0	35.0	40.0	29.0	41.0
32	35.8444968070778	38.0	35.0	40.0	29.0	41.0
33	35.66403378496105	38.0	34.0	40.0	27.0	41.0
34	35.551455966989415	38.0	34.0	40.0	27.0	41.0
35	35.31315562764637	38.0	34.0	40.0	26.0	41.0
36	35.160292576213905	38.0	34.0	40.0	25.0	41.0
37	35.009853601267764	38.0	33.0	40.0	25.0	41.0
38	34.918120697713654	38.0	33.0	40.0	25.0	41.0
39	34.82586094125521	38.0	33.0	40.0	24.0	41.0
40	34.688476440963434	38.0	33.0	40.0	24.0	41.0
41	34.485510162314945	38.0	33.0	40.0	23.0	41.0
42	34.2628162845946	38.0	33.0	40.0	22.0	41.0
43	33.92313444290491	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	5.0
14	8.0
15	15.0
16	47.0
17	106.0
18	273.0
19	565.0
20	1373.0
21	2620.0
22	4875.0
23	8366.0
24	13268.0
25	20336.0
26	28990.0
27	40103.0
28	54025.0
29	69657.0
30	87823.0
31	108241.0
32	132592.0
33	161924.0
34	197329.0
35	238638.0
36	302311.0
37	397633.0
38	510666.0
39	510850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.294447163982625	21.55018515906533	13.749235994147924	23.40613168280413
2	19.202889532359276	23.690369516097363	34.63352609788678	22.473214853656582
3	19.63705919282263	24.837795680635875	32.70629540822112	22.81884971832038
4	13.79338239564579	16.55092496563702	36.58039277506285	33.07529986365434
5	12.249848581645606	38.97746977855584	35.20995295659894	13.562728683199616
6	32.08509988080126	37.940634325365544	13.480727693103342	16.49353810072985
7	26.10480086066469	33.01137919304388	21.483498856411153	19.400321089880276
8	27.162897110191075	34.32394816099297	18.78361971453146	19.729535014284487
9	24.617651369955833	14.293719802755122	21.567193796134198	39.521435031154844
10	16.525031735627703	27.626209618315123	33.504214823234626	22.344543822822548
11	33.38263072451262	22.648106510021268	23.344008673022092	20.625254092444017
12	21.171431850677994	27.505870054012792	30.537694181939866	20.785003913369344
13	31.181360469714946	21.139799934177958	26.017718021688086	21.66112157441901
14	20.574954159648875	23.151036697171588	27.486648911308947	28.78736023187059
15	22.84457009632696	29.684738689256353	25.425941905133286	22.044749309283397
16	22.022693393734738	28.48085906062542	26.39685160448143	23.099595941158412
17	21.949265862973995	26.957272367740558	26.83451287539998	24.258948893885464
18	22.82728489605372	25.92206172337595	29.270066734701217	21.980586645869113
19	22.94924926918173	25.094238911889725	30.209759362355875	21.74675245657266
20	23.677613038295707	24.803882117699768	30.100171192623502	21.41833365138102
21	22.493853382782834	25.71076743523581	30.57050149205849	21.224877689922867
22	21.418748496187575	26.164089097601924	29.963756392067058	22.453406014143443
23	22.37534604970947	26.25255475260039	30.942098727532695	20.43000047015745
24	22.37949449777505	26.466061546375503	28.317583058844352	22.836860897005096
25	22.186660803526735	26.425303044131194	29.72757141553345	21.660464736808628
26	21.924167752177244	27.215202126217914	29.643530771804933	21.217099349799906
27	21.10796059527464	27.206939800487305	29.079964102096074	22.605135502141984
28	21.486713903661975	26.75016109806655	29.457438305663185	22.305686692608294
29	21.965859655236308	26.852282061280874	28.848999256044983	22.332859027437834
30	21.716572496895576	26.887025313830097	30.556223916632785	20.840178272641538
31	22.231671465038264	26.81017531341525	30.20301813424931	20.755135087297173
32	21.725837364242036	26.51833199200179	29.33208603328162	22.423744610474554
33	21.37145618823998	26.631722905794277	29.99545744936819	22.001363456597552
34	20.89469579430335	26.98143707772256	30.493858914046925	21.63000821392717
35	20.478468171723623	28.917172085922655	29.35379624482481	21.25056349752891
36	22.688761301063938	26.717768632754485	29.102884277658397	21.49058578852318
37	21.443777466183235	27.303218366009276	29.294093163081026	21.958911004726463
38	21.395033201412687	25.869894988951298	30.697616854867928	22.037454954768087
39	20.83692865499017	25.84808106620646	31.399361415561106	21.91562886324226
40	19.85958886114038	26.521719891255348	30.97580486806552	22.642886379538748
41	21.078610325210672	25.223947054740155	31.213199808618263	22.48424281143091
42	19.874246710972095	27.14377967868887	30.59639472206781	22.385578888271233
43	20.237132205508587	26.61481797992704	30.071235767366094	23.076814047198276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	320.0
2	487.0
3	1157.0
4	1827.0
5	1827.0
6	2920.5
7	4014.0
8	4605.5
9	5197.0
10	8045.5
11	10894.0
12	10894.0
13	21210.0
14	31526.0
15	49781.5
16	68037.0
17	62271.0
18	56505.0
19	56505.0
20	60004.5
21	63504.0
22	51030.5
23	38557.0
24	43165.0
25	47773.0
26	47773.0
27	52815.5
28	57858.0
29	63750.0
30	69642.0
31	78672.0
32	87702.0
33	87702.0
34	99285.0
35	110868.0
36	122756.0
37	134644.0
38	144072.0
39	153500.0
40	153500.0
41	158208.0
42	162916.0
43	174509.5
44	186103.0
45	192187.0
46	198271.0
47	198271.0
48	246514.5
49	294758.0
50	279189.0
51	263620.0
52	242226.0
53	220832.0
54	220832.0
55	197290.0
56	173748.0
57	148074.0
58	122400.0
59	108116.0
60	93832.0
61	93832.0
62	82549.5
63	71267.0
64	61888.5
65	52510.0
66	44810.0
67	37110.0
68	37110.0
69	31898.5
70	26687.0
71	22452.5
72	18218.0
73	14886.5
74	11555.0
75	11555.0
76	9363.0
77	7171.0
78	5619.5
79	4068.0
80	3232.5
81	2397.0
82	2397.0
83	1807.5
84	1218.0
85	922.5
86	627.0
87	485.0
88	343.0
89	343.0
90	263.0
91	183.0
92	131.0
93	79.0
94	55.5
95	32.0
96	32.0
97	20.5
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2892648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.24972718424008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98122103472627	37.79090873753221
2	11.300757386965158	10.67915407018779
3	3.61784635999737	5.128267605131153
4	1.521409849962135	2.8754480138450607
5	0.8304842486595514	1.9620077089986203
6	0.5058822554883923	1.4341679135504746
7	0.342156814878782	1.1316771310075693
8	0.2538354221621695	0.9594923557487133
9	0.18541057192595123	0.7884539046517607
>10	1.2123835118247968	11.06467033593449
>50	0.13344685518380858	4.362166651069985
>100	0.09822432675444868	9.239879545369293
>500	0.010533107349555378	3.3832237535024463
>1k	0.005671673188217288	4.5075426852647125
>5k	4.4194856012082765E-4	1.351723355232955
>10k+	2.946323734138851E-4	3.3412162329727693
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33135	1.1454902221079093	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27894	0.9643067528437611	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22432	0.7754832250588388	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12542	0.43358196365406365	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7475	0.25841374408500445	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6928	0.23950373498607502	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6847	0.23670353254180942	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6060	0.2094966273117227	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5948	0.2056247424505159	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5581	0.1929374054499545	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3745	0.1294661500466009	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3726	0.1288093124362176	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3597	0.12434973076572053	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3451	0.11930245228593316	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3324	0.11491201141652907	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3240	0.11200809777062401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.457040054648889E-5	0.0
2	0.0	0.0	0.0	3.457040054648889E-5	0.0
3	0.0	0.0	0.0	3.457040054648889E-5	0.0
4	0.0	0.0	0.0	3.457040054648889E-5	0.0
5	0.0	0.0	0.0	3.457040054648889E-5	0.0
6	0.0	0.0	0.0	3.457040054648889E-5	3.457040054648889E-5
7	0.0	0.0	0.0	1.3828160218595556E-4	3.457040054648889E-5
8	0.0	0.0	0.0	1.7285200273244446E-4	3.457040054648889E-5
9	0.0	0.0	0.0	7.259784114762667E-4	3.457040054648889E-5
10	3.457040054648889E-5	0.0	0.0	8.642600136622223E-4	3.457040054648889E-5
11	6.914080109297778E-5	0.0	0.0	0.0010716824169411557	3.457040054648889E-5
12	6.914080109297778E-5	0.0	0.0	0.0013136752207665778	3.457040054648889E-5
13	6.914080109297778E-5	0.0	0.0	0.0013482456213130668	3.457040054648889E-5
14	6.914080109297778E-5	0.0	0.0	0.0014865272234990223	3.457040054648889E-5
15	6.914080109297778E-5	0.0	0.0	0.0016939496267779556	3.457040054648889E-5
16	1.0371120163946668E-4	0.0	0.0	0.0021433648338823113	3.457040054648889E-5
17	1.0371120163946668E-4	0.0	0.0	0.002523639239893689	3.457040054648889E-5
18	1.0371120163946668E-4	0.0	0.0	0.002834772844812089	3.457040054648889E-5
19	1.0371120163946668E-4	0.0	0.0	0.0033878992535559113	3.457040054648889E-5
20	1.0371120163946668E-4	0.0	0.0	0.006153531297275023	3.457040054648889E-5
21	1.0371120163946668E-4	0.0	0.0	0.010025416158481779	6.914080109297778E-5
22	1.0371120163946668E-4	0.0	0.0	0.015452969044280535	6.914080109297778E-5
23	1.0371120163946668E-4	0.0	0.0	0.01846059389182507	6.914080109297778E-5
24	1.0371120163946668E-4	0.0	0.0	0.024130139581449248	6.914080109297778E-5
25	1.0371120163946668E-4	0.0	0.0	0.029281129262876092	6.914080109297778E-5
26	1.0371120163946668E-4	0.0	0.0	0.037647166195126404	6.914080109297778E-5
27	1.0371120163946668E-4	0.0	0.0	0.09257953266349725	6.914080109297778E-5
28	1.0371120163946668E-4	0.0	0.0	0.2032048144122617	6.914080109297778E-5
29	1.0371120163946668E-4	0.0	0.0	0.3433532182277277	6.914080109297778E-5
30	1.0371120163946668E-4	0.0	0.0	0.538537699713204	6.914080109297778E-5
31	1.0371120163946668E-4	0.0	0.0	0.7677740257369718	6.914080109297778E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9690	0.0	22.891127	1
GTATTGG	1095	0.0	20.273973	1
GTATTAG	910	0.0	16.263735	1
ATTGGAC	1465	0.0	16.163822	3
TAATACT	920	0.0	15.88587	4
TTAACGG	585	0.0	15.811965	35
TGGACCC	1645	0.0	15.519757	5
AATACTG	965	0.0	15.145078	5
TTGGACC	1835	0.0	15.021799	4
AGTACCG	325	0.0	14.8	5
GTATCAA	15430	0.0	14.387557	2
GGACCCT	1740	0.0	14.247127	6
ATACTGG	820	0.0	14.213415	6
TAACGGC	640	0.0	14.1640625	36
ACCGTTA	510	0.0	14.1470585	8
GGTAAAC	750	0.0	14.060001	35
CTAATAC	900	0.0	13.9777775	3
GTGACAC	795	0.0	13.962263	24
ATTAGAG	875	0.0	13.954286	3
TATTAGA	975	0.0	13.661538	2
>>END_MODULE