FastQCFastQC Report
Wed 25 May 2016
SRR2088459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301387
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147561.1338671740227926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121310.9321593038811669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98100.7538111261292759No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.41156089618230396No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG31880.24496940571866788No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29620.2276033186131412No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA28480.21884343396699057No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26030.20001736608710555No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA24610.1891059308261109No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23610.18142182148738228No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15590.11979526459077892No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA15200.11679846194867477No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15120.11618373320157648No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.11026696901075546No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13900.1068091198083276No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13360.10265970076541414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.022.990291
GAGTCGC450.003826075220.55555726
TATACCG450.003826075220.5555575
CTTACTC1254.129106E-1019.243
ATAGACG701.2193544E-418.53
TCTATAC1002.8756403E-718.53
GTATTGG4200.018.0595231
AGACGCG650.001580086117.07692321
TGTTACG1554.0017767E-1016.70967916
ACCGTTA2550.016.6862748
CCTAGAC803.382994E-416.18752
TAGCGCA803.382994E-416.18754
GGACCGT3100.015.51612956
ACCGTTC855.364826E-415.2352948
TTTAGAC1101.4520825E-515.1363643
GTATCAA63700.014.8116182
ATTAGAC1252.9592902E-614.7999993
TTGGACC6750.014.5259264
GACCGTT2950.014.4237297
ATTGGAC4750.014.4105263
Produced by FastQC (version 0.11.5)