##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1301387
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.388774438349238	31.0	31.0	34.0	30.0	34.0
2	31.52446274628531	31.0	31.0	34.0	30.0	34.0
3	31.665231018905214	31.0	31.0	34.0	30.0	34.0
4	35.38369293684354	37.0	35.0	37.0	33.0	37.0
5	35.24742985752893	37.0	35.0	37.0	33.0	37.0
6	35.32117271803084	37.0	35.0	37.0	33.0	37.0
7	35.155467973784894	37.0	35.0	37.0	32.0	37.0
8	35.19568737047473	37.0	35.0	37.0	32.0	37.0
9	36.743409147317436	39.0	35.0	39.0	32.0	39.0
10	36.584373441566576	38.0	35.0	39.0	32.0	39.0
11	36.61542262217158	38.0	35.0	39.0	32.0	39.0
12	36.469139464279266	38.0	35.0	39.0	32.0	39.0
13	36.48773193523525	38.0	35.0	39.0	32.0	39.0
14	37.438987787645026	39.0	36.0	41.0	32.0	41.0
15	37.45829718600232	39.0	36.0	41.0	32.0	41.0
16	37.326130505376184	39.0	36.0	41.0	32.0	41.0
17	37.38581067737729	39.0	36.0	41.0	32.0	41.0
18	37.25863636258853	39.0	36.0	40.0	31.0	41.0
19	37.336404159562065	39.0	36.0	40.0	31.0	41.0
20	37.243282743718815	39.0	36.0	40.0	31.0	41.0
21	37.08259418604919	39.0	36.0	40.0	31.0	41.0
22	36.97584884434838	39.0	36.0	40.0	31.0	41.0
23	36.775056151628995	39.0	35.0	40.0	30.0	41.0
24	36.9934008868999	39.0	36.0	40.0	31.0	41.0
25	36.95322836327703	39.0	36.0	40.0	31.0	41.0
26	36.75508976192324	39.0	35.0	40.0	30.0	41.0
27	36.69306824180663	39.0	35.0	40.0	30.0	41.0
28	36.50673243239713	39.0	35.0	40.0	30.0	41.0
29	36.3261036109935	38.0	35.0	40.0	30.0	41.0
30	36.14050701290239	38.0	35.0	40.0	29.0	41.0
31	35.879986506704	38.0	35.0	40.0	28.0	41.0
32	35.738164742693755	38.0	34.0	40.0	28.0	41.0
33	35.55200413097718	38.0	34.0	40.0	27.0	41.0
34	35.42609154694184	38.0	34.0	40.0	27.0	41.0
35	35.18574490140135	38.0	34.0	40.0	26.0	41.0
36	35.02335123986946	38.0	33.0	40.0	25.0	41.0
37	34.85943228263383	38.0	33.0	40.0	25.0	41.0
38	34.762485717161766	38.0	33.0	40.0	24.0	41.0
39	34.660288599778546	38.0	33.0	40.0	24.0	41.0
40	34.53112871113666	38.0	33.0	40.0	23.0	41.0
41	34.31939461512986	38.0	33.0	40.0	22.0	41.0
42	34.09178053876364	38.0	33.0	40.0	21.0	41.0
43	33.74720202368704	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	5.0
15	14.0
16	22.0
17	43.0
18	152.0
19	289.0
20	638.0
21	1390.0
22	2362.0
23	3923.0
24	6170.0
25	9550.0
26	13317.0
27	18736.0
28	24981.0
29	32417.0
30	40227.0
31	49519.0
32	61205.0
33	74565.0
34	89439.0
35	108867.0
36	137412.0
37	178530.0
38	227781.0
39	219829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.270506006284066	21.3673565203894	13.772613373270213	23.589524100056323
2	19.35826929268542	23.713776148063566	34.227481909685594	22.700472649565427
3	19.831687269044487	24.60836015727835	32.41533840433322	23.14461416934394
4	14.098112244858754	16.57631434769212	35.986144014040406	33.33942939340873
5	12.656573332913268	38.66259613781297	34.80678691273234	13.874043616541428
6	32.65615839100898	37.31103814622399	13.562452982855982	16.47035047991105
7	26.39184193479726	32.796393386440776	21.264158931970275	19.547605746791692
8	27.358656571796093	34.13527259762085	18.83236884954283	19.673701981040228
9	25.02545361218454	14.2833761210155	21.122771320137666	39.56839894666229
10	16.625800011833526	27.61715000995092	33.171531604357504	22.585518373858047
11	33.70327197059753	22.5658470539509	23.043337608259495	20.68754336719208
12	21.295817462445836	27.287117513852525	30.389192453897262	21.02787256980437
13	31.60727746627252	20.928593877147996	25.68674806187552	21.777380594703956
14	20.84330026348811	22.953126164622823	27.101238908948684	29.102334662940386
15	23.06784991705004	29.616478418794717	24.90873199132925	22.40693967282599
16	22.249184908101892	28.220352593041113	26.115137157509643	23.415325341347348
17	22.19547298382418	26.829144597264303	26.616141086394745	24.359241332516767
18	23.165591787838668	25.62673516794005	28.918069720997675	22.289603323223606
19	23.15867608943381	25.042666017103294	29.8843464703428	21.914311423120104
20	23.97980001337035	24.799002909972206	29.584819888319153	21.636377188338287
21	22.937757945945364	25.45829949123512	29.921383877355467	21.68255868546405
22	21.939438460657744	25.906744112243324	29.524960676570462	22.628856750528474
23	22.71699348464369	26.035452943667025	30.297521029486234	20.95003254220305
24	22.63062409567638	26.047824359702382	28.216664220558528	23.104887324062712
25	22.58536469167127	26.173075341923656	29.300892048253136	21.94066791815194
26	22.282841307005526	26.763138098044625	29.331090597954336	21.622929996995513
27	21.494835894318907	26.846894889836765	28.944042010562576	22.71422720528175
28	21.987310461838025	26.529003286493563	28.927982222044633	22.555704029623776
29	22.132002240686283	26.646185953909175	28.511887701352478	22.709924104052064
30	22.024732074317633	26.782502053578224	29.99177031889822	21.200995553205924
31	22.5972750611463	26.550134587175066	29.7749247533593	21.07766559831933
32	22.14168421845308	26.44793593296998	28.85260110943171	22.557778739145235
33	21.893103281345212	26.39122720605016	29.39548343421288	22.320186078391746
34	21.328935973695756	26.705737801284325	29.89187689749475	22.07344932752517
35	20.97608167286134	28.424673060357907	29.06998456262434	21.52926070415641
36	22.90448575250867	26.41958156951007	28.84084442214345	21.83508825583781
37	21.919382935283664	27.001499169731986	28.872195588245464	22.20692230673889
38	21.777534276890734	25.728011729024498	30.191326638425004	22.30312735565977
39	21.283215523130323	25.620280516095516	30.897496286654164	22.199007674119997
40	20.496362726844513	26.067726202889684	30.552787141718795	22.883123928547004
41	21.50351893787167	25.018922119246618	30.721453341703892	22.75610560117782
42	20.33622588822541	26.702510475362057	30.26839825509245	22.692865381320086
43	20.638211385237444	26.44163496331222	29.71521922379738	23.204934427652958
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	154.5
2	215.0
3	508.5
4	802.0
5	802.0
6	1258.5
7	1715.0
8	1980.0
9	2245.0
10	3544.0
11	4843.0
12	4843.0
13	9368.5
14	13894.0
15	21956.5
16	30019.0
17	27259.0
18	24499.0
19	24499.0
20	26226.0
21	27953.0
22	22920.0
23	17887.0
24	20037.0
25	22187.0
26	22187.0
27	24376.0
28	26565.0
29	29024.0
30	31483.0
31	34929.5
32	38376.0
33	38376.0
34	42837.5
35	47299.0
36	51877.0
37	56455.0
38	60366.0
39	64277.0
40	64277.0
41	66730.0
42	69183.0
43	73674.5
44	78166.0
45	82071.0
46	85976.0
47	85976.0
48	107375.0
49	128774.0
50	122305.5
51	115837.0
52	107574.0
53	99311.0
54	99311.0
55	89795.0
56	80279.0
57	69856.5
58	59434.0
59	53509.0
60	47584.0
61	47584.0
62	42122.0
63	36660.0
64	32360.0
65	28060.0
66	24358.5
67	20657.0
68	20657.0
69	17634.0
70	14611.0
71	12441.0
72	10271.0
73	8452.5
74	6634.0
75	6634.0
76	5353.0
77	4072.0
78	3228.5
79	2385.0
80	1852.0
81	1319.0
82	1319.0
83	985.5
84	652.0
85	505.5
86	359.0
87	271.0
88	183.0
89	183.0
90	140.0
91	97.0
92	70.0
93	43.0
94	32.5
95	22.0
96	22.0
97	14.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1301387.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.51452344692876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.83124013351731	47.376825861107804
2	9.471280348390714	10.705297906431255
3	2.620810756172253	4.443416127889797
4	1.145256764225142	2.588945610182223
5	0.6323481053335275	1.7868425912746309
6	0.40130937040265	1.3607884693855672
7	0.287698480300109	1.13813997573964
8	0.21809244834612224	0.9860312628524021
9	0.1620914188076753	0.8244467359877087
>10	1.0683960344029466	11.493412627319314
>50	0.09490816079646934	3.7119094307419123
>100	0.058792632609002346	6.119893384363424
>500	0.004910745281828891	1.8604426627861055
>1k	0.002318963049752532	2.3616898427237536
>5k	2.728191823238273E-4	1.1691655999591462
>10k+	2.728191823238273E-4	2.072751911255543
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14756	1.1338671740227926	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12131	0.9321593038811669	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9810	0.7538111261292759	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5356	0.41156089618230396	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3188	0.24496940571866788	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2962	0.2276033186131412	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2848	0.21884343396699057	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2603	0.20001736608710555	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2461	0.1891059308261109	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2361	0.18142182148738228	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1559	0.11979526459077892	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1520	0.11679846194867477	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1512	0.11618373320157648	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1435	0.11026696901075546	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1390	0.1068091198083276	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1336	0.10265970076541414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.684109338728603E-5	0.0
5	0.0	0.0	0.0	7.684109338728603E-5	0.0
6	7.684109338728603E-5	0.0	0.0	7.684109338728603E-5	0.0
7	7.684109338728603E-5	0.0	0.0	7.684109338728603E-5	0.0
8	7.684109338728603E-5	0.0	0.0	7.684109338728603E-5	0.0
9	7.684109338728603E-5	0.0	0.0	3.073643735491441E-4	0.0
10	7.684109338728603E-5	0.0	0.0	4.6104656032371614E-4	0.0
11	7.684109338728603E-5	0.0	0.0	5.378876537110022E-4	0.0
12	7.684109338728603E-5	0.0	0.0	6.915698404855742E-4	0.0
13	7.684109338728603E-5	0.0	0.0	6.915698404855742E-4	0.0
14	7.684109338728603E-5	0.0	0.0	7.684109338728603E-4	0.0
15	7.684109338728603E-5	0.0	0.0	9.989342140347183E-4	0.0
16	1.5368218677457206E-4	0.0	0.0	0.0019210273346821508	0.0
17	2.3052328016185807E-4	0.0	0.0	0.002612597175167725	0.0
18	2.3052328016185807E-4	0.0	0.0	0.0029968026421041552	0.0
19	2.3052328016185807E-4	0.0	0.0	0.004149419042913445	0.0
20	2.3052328016185807E-4	0.0	0.0	0.006454651844532026	0.0
21	2.3052328016185807E-4	0.0	0.0	0.010757753074220043	0.0
22	2.3052328016185807E-4	0.0	0.0	0.016290311798104636	0.0
23	2.3052328016185807E-4	0.0	0.0	0.019978684280694367	0.0
24	2.3052328016185807E-4	0.0	0.0	0.025357560817804388	0.0
25	2.3052328016185807E-4	0.0	0.0	0.03127432500862541	0.0
26	2.3052328016185807E-4	0.0	0.0	0.038497387787030296	0.0
27	2.3052328016185807E-4	0.0	0.0	0.09044196691683565	0.0
28	2.3052328016185807E-4	0.0	0.0	0.19156484581450406	0.0
29	2.3052328016185807E-4	0.0	0.0	0.3193515841175607	0.0
30	2.3052328016185807E-4	0.0	0.0	0.4965471454686423	0.0
31	2.3052328016185807E-4	0.0	0.0	0.7060928071357713	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4120	0.0	22.99029	1
GAGTCGC	45	0.0038260752	20.555557	26
TATACCG	45	0.0038260752	20.555557	5
CTTACTC	125	4.129106E-10	19.24	3
ATAGACG	70	1.2193544E-4	18.5	3
TCTATAC	100	2.8756403E-7	18.5	3
GTATTGG	420	0.0	18.059523	1
AGACGCG	65	0.0015800861	17.076923	21
TGTTACG	155	4.0017767E-10	16.709679	16
ACCGTTA	255	0.0	16.686274	8
CCTAGAC	80	3.382994E-4	16.1875	2
TAGCGCA	80	3.382994E-4	16.1875	4
GGACCGT	310	0.0	15.5161295	6
ACCGTTC	85	5.364826E-4	15.235294	8
TTTAGAC	110	1.4520825E-5	15.136364	3
GTATCAA	6370	0.0	14.811618	2
ATTAGAC	125	2.9592902E-6	14.799999	3
TTGGACC	675	0.0	14.525926	4
GACCGTT	295	0.0	14.423729	7
ATTGGAC	475	0.0	14.410526	3
>>END_MODULE