FastQCFastQC Report
Wed 25 May 2016
SRR2088458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936645
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115931.2377154631690768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92470.9872470359634653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82230.8779206636452443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39270.4192623672789584No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG24800.2647748079581912No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG24720.2639206956744551No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22410.23925820348157523No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22150.23648233855943288No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA21170.22601946308366563No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG20070.21427541918229426No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13140.14028794260365454No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12540.13388210047563376No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12300.13131976362442546No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC11340.12107041621959226No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA10670.11391722584330242No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.10153259772912897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGC405.938975E-527.7525
TCGTTCC400.00193092223.12528
TATTGCG400.00193092223.1259
GGTATCA35800.020.7220651
GTATTGG3600.020.5555551
CGCCTAT450.003825286920.55555534
TCTATCG450.003825286920.55555529
GCTTTAT851.2448054E-619.5882361
TTGGACC5950.018.9663874
TCTATGC609.235072E-418.58
ATTGGAC4600.018.0978263
TTAGAAC752.0667512E-417.2666663
CGAACAA650.00157963917.07692314
CACCTAA650.00157963917.0769231
GATACTG2550.016.68627436
TGGACCC6200.016.4112915
GGACCCT6350.016.314966
ATGTGAC803.3817298E-416.187511
GATCATC855.362835E-415.23529411
CAATGCG855.362835E-415.23529419
Produced by FastQC (version 0.11.5)