##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	936645
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420849948486353	31.0	31.0	34.0	30.0	34.0
2	31.541191166343705	31.0	31.0	34.0	30.0	34.0
3	31.702041862178305	31.0	31.0	34.0	30.0	34.0
4	35.414457985682944	37.0	35.0	37.0	33.0	37.0
5	35.291423111210754	37.0	35.0	37.0	33.0	37.0
6	35.36422443935536	37.0	35.0	37.0	33.0	37.0
7	35.20101852889835	37.0	35.0	37.0	32.0	37.0
8	35.239152507086466	37.0	35.0	37.0	32.0	37.0
9	36.795307720641226	39.0	37.0	39.0	32.0	39.0
10	36.63832828873266	39.0	35.0	39.0	32.0	39.0
11	36.67293798611	39.0	35.0	39.0	32.0	39.0
12	36.521454766747276	38.0	35.0	39.0	32.0	39.0
13	36.52781149741898	38.0	35.0	39.0	32.0	39.0
14	37.47086889910265	39.0	36.0	41.0	32.0	41.0
15	37.499421872747945	39.0	36.0	41.0	32.0	41.0
16	37.362241831216735	39.0	36.0	41.0	32.0	41.0
17	37.43587805411869	39.0	36.0	41.0	32.0	41.0
18	37.31616567643024	39.0	36.0	41.0	32.0	41.0
19	37.401885452866345	39.0	36.0	41.0	32.0	41.0
20	37.29985960529336	39.0	36.0	41.0	31.0	41.0
21	37.14544464551671	39.0	36.0	40.0	31.0	41.0
22	37.046479722840566	39.0	36.0	40.0	31.0	41.0
23	36.828873265751696	39.0	35.0	40.0	30.0	41.0
24	37.026401678330636	39.0	36.0	40.0	31.0	41.0
25	37.00085838284515	39.0	36.0	41.0	31.0	41.0
26	36.81215508543792	39.0	36.0	40.0	30.0	41.0
27	36.73607610140448	39.0	35.0	40.0	30.0	41.0
28	36.531050718255045	39.0	35.0	40.0	30.0	41.0
29	36.359649600435596	39.0	35.0	40.0	30.0	41.0
30	36.16826011989601	38.0	35.0	40.0	30.0	41.0
31	35.89681149208078	38.0	35.0	40.0	29.0	41.0
32	35.751849420004376	38.0	34.0	40.0	28.0	41.0
33	35.52461071163568	38.0	34.0	40.0	27.0	41.0
34	35.4001718900971	38.0	34.0	40.0	27.0	41.0
35	35.161537188582656	38.0	34.0	40.0	26.0	41.0
36	35.009271388839956	38.0	33.0	40.0	25.0	41.0
37	34.83302852201208	38.0	33.0	40.0	25.0	41.0
38	34.72691895008248	38.0	33.0	40.0	24.0	41.0
39	34.61088459341586	38.0	33.0	40.0	24.0	41.0
40	34.487103438335765	38.0	33.0	40.0	23.0	41.0
41	34.26670083115802	38.0	33.0	40.0	22.0	41.0
42	34.0095436371304	38.0	33.0	40.0	21.0	41.0
43	33.65115385231331	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	2.0
15	10.0
16	15.0
17	50.0
18	95.0
19	209.0
20	496.0
21	935.0
22	1713.0
23	2849.0
24	4511.0
25	6696.0
26	9634.0
27	13356.0
28	17557.0
29	23327.0
30	28549.0
31	35334.0
32	43088.0
33	52841.0
34	64544.0
35	77815.0
36	99364.0
37	131122.0
38	164074.0
39	158455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.89986601113549	20.940164096322516	13.704124828510267	23.45584506403173
2	19.238985955191133	23.75937521686445	34.49086900586668	22.510769822077734
3	19.83825248626748	24.750679286175657	32.35420036406536	23.056867863491505
4	13.947973885516927	16.425006272387083	35.93955020311858	33.68746963897741
5	12.897202248450586	38.36864553806405	34.594430120269685	14.139722093215678
6	32.64182267561349	37.20982869710509	13.477678309284736	16.67067031799668
7	26.355556267315794	32.76449455236509	21.246470114077372	19.633479066241748
8	27.616759818287612	33.911247057316274	18.68626854357841	19.785724580817707
9	25.10075855847199	14.184883280218225	21.213907083259933	39.50045107804985
10	16.70280629267225	27.379743659550844	32.84253906229148	23.07491098548543
11	33.6515969230605	22.46443423068505	23.05985725648458	20.82411158976987
12	21.574128938925632	27.12756700777776	30.33668038584522	20.96162366745138
13	31.95842608458915	20.824431881876272	25.600307480422146	21.616834553112437
14	20.96386571219619	22.81152410998831	27.253121513486967	28.971488664328533
15	23.239327600104627	29.364487078882608	24.873778219069123	22.52240710194364
16	22.25827287819825	28.104030876159058	26.00013879324611	23.637557452396585
17	22.386282956723196	26.492320996749037	26.436269878128854	24.685126168398913
18	23.594318018032446	25.334785324215687	28.726678731002675	22.344217926749195
19	23.365629454062102	24.707866907953388	29.868093034180504	22.058410603804003
20	24.234688702763588	24.509926386197545	29.511180863614285	21.744204047424585
21	23.10234934260045	25.17880306839838	30.03624639004105	21.682601198960118
22	21.86559475575058	25.67600318156826	29.682110084396967	22.776291978284195
23	22.830528108301436	25.782019868787	30.305825579595258	21.081626443316303
24	22.766576451056697	25.852270604124293	28.298341420709022	23.08281152410999
25	22.620843542644227	25.950600280789416	29.2897522540557	22.138803922510665
26	22.3454990951748	26.804178744348178	29.23434171964832	21.615980440828704
27	21.546904109881545	26.721329852825775	28.86579226921619	22.865973768076486
28	21.917268548916613	26.487303087082086	28.972449540647737	22.622978823353566
29	22.156419988362718	26.407550352588228	28.48357702224429	22.952452636804765
30	22.013996765049725	26.580401326009323	30.079379060369725	21.32622284857123
31	22.674545852484133	26.323206764569285	29.77947888474289	21.222768498203695
32	22.224428678955206	26.359613300663536	28.75817412146544	22.657783898915813
33	21.99456571059473	26.317655034725	29.409327973778755	22.278451280901514
34	21.2340854859632	26.875710648111077	29.854320473605263	22.035883392320464
35	20.887315898766342	28.720166124839185	28.933907723844143	21.458610252550326
36	22.97423250004004	26.449722146597697	28.814011712014693	21.762033641347575
37	21.83762257845822	27.276823129360643	28.682691948390264	22.202862343790873
38	21.796198132697022	25.854405884833636	30.08407667793027	22.265319304539073
39	21.35302062147345	25.66009534028367	30.81647796123398	22.1704060770089
40	20.32317473535865	26.261710680140286	30.511239583833788	22.903875000667277
41	21.57348835471283	25.17495956312157	30.557148119084605	22.694403963080997
42	20.31100363531541	26.82830741636372	30.18710397215594	22.67358497616493
43	20.695033870890253	26.63698626480684	29.49377832583316	23.174201538469752
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	115.0
2	173.0
3	388.5
4	604.0
5	604.0
6	995.0
7	1386.0
8	1552.5
9	1719.0
10	2735.0
11	3751.0
12	3751.0
13	7040.5
14	10330.0
15	16846.0
16	23362.0
17	21375.0
18	19388.0
19	19388.0
20	20143.5
21	20899.0
22	16782.5
23	12666.0
24	14111.0
25	15556.0
26	15556.0
27	17171.5
28	18787.0
29	20249.0
30	21711.0
31	23995.0
32	26279.0
33	26279.0
34	29091.0
35	31903.0
36	34948.5
37	37994.0
38	40771.5
39	43549.0
40	43549.0
41	44739.0
42	45929.0
43	49574.5
44	53220.0
45	56805.0
46	60390.0
47	60390.0
48	77933.0
49	95476.0
50	90863.5
51	86251.0
52	80147.0
53	74043.0
54	74043.0
55	66443.5
56	58844.0
57	51451.0
58	44058.0
59	39492.0
60	34926.0
61	34926.0
62	31072.0
63	27218.0
64	23710.5
65	20203.0
66	17515.5
67	14828.0
68	14828.0
69	12861.5
70	10895.0
71	9315.0
72	7735.0
73	6494.5
74	5254.0
75	5254.0
76	4231.0
77	3208.0
78	2504.5
79	1801.0
80	1448.5
81	1096.0
82	1096.0
83	814.5
84	533.0
85	417.5
86	302.0
87	219.5
88	137.0
89	137.0
90	118.5
91	100.0
92	71.5
93	43.0
94	37.0
95	31.0
96	31.0
97	18.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	936645.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.8308752288234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.19961146503661	50.123677116438
2	8.542548143251318	10.051311679036704
3	2.378196697747053	4.197341795842701
4	1.0678276272769627	2.512849356248861
5	0.6033583952584426	1.7748051234856275
6	0.3800232689751504	1.3414260912676002
7	0.2965534718785578	1.2212550211933275
8	0.21727524122561195	1.0225994085485186
9	0.17152440276578682	0.9081837664031209
>10	1.0047607879879445	11.06267936384093
>50	0.08540362010152151	3.424036539968931
>100	0.04580047075918432	4.961603701202843
>500	0.004379327837475296	1.7601084794002637
>1k	0.002189663918737648	2.518213266026555
>5k	3.649439864562747E-4	1.8754022404929622
>10k+	1.8247199322813734E-4	1.2445070506030287
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11593	1.2377154631690768	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9247	0.9872470359634653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8223	0.8779206636452443	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3927	0.4192623672789584	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2480	0.2647748079581912	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2472	0.2639206956744551	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2241	0.23925820348157523	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2215	0.23648233855943288	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2117	0.22601946308366563	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2007	0.21427541918229426	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1314	0.14028794260365454	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1254	0.13388210047563376	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1230	0.13131976362442546	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1134	0.12107041621959226	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1067	0.11391722584330242	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	951	0.10153259772912897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.0676403546701258E-4	0.0
10	1.0676403546701258E-4	0.0	0.0	1.0676403546701258E-4	0.0
11	2.1352807093402517E-4	0.0	0.0	2.1352807093402517E-4	0.0
12	2.1352807093402517E-4	0.0	0.0	7.473482482690881E-4	0.0
13	2.1352807093402517E-4	0.0	0.0	7.473482482690881E-4	0.0
14	2.1352807093402517E-4	0.0	0.0	8.541122837361007E-4	0.0
15	2.1352807093402517E-4	0.0	0.0	8.541122837361007E-4	0.0
16	2.1352807093402517E-4	0.0	0.0	0.0013879324610711636	0.0
17	2.1352807093402517E-4	0.0	0.0	0.002348808780274277	0.0
18	2.1352807093402517E-4	0.0	0.0	0.0026691008866753145	0.0
19	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.00373674124134544	0.0
20	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.007046426340822831	0.0
21	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.01281168425604151	0.0
22	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.01943105445499629	0.0
23	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.024128672015544844	0.0
24	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.02989392993076352	0.0
25	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.03448478345584506	0.0
26	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.04452060278974425	0.0
27	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.09971760912618975	0.0
28	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.21224690250842101	0.0
29	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.3522145530056745	0.0
30	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.5505821309033839	0.0
31	2.1352807093402517E-4	0.0	1.0676403546701258E-4	0.7695551676462267	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTAGC	40	5.938975E-5	27.75	25
TCGTTCC	40	0.001930922	23.125	28
TATTGCG	40	0.001930922	23.125	9
GGTATCA	3580	0.0	20.722065	1
GTATTGG	360	0.0	20.555555	1
CGCCTAT	45	0.0038252869	20.555555	34
TCTATCG	45	0.0038252869	20.555555	29
GCTTTAT	85	1.2448054E-6	19.588236	1
TTGGACC	595	0.0	18.966387	4
TCTATGC	60	9.235072E-4	18.5	8
ATTGGAC	460	0.0	18.097826	3
TTAGAAC	75	2.0667512E-4	17.266666	3
CGAACAA	65	0.001579639	17.076923	14
CACCTAA	65	0.001579639	17.076923	1
GATACTG	255	0.0	16.686274	36
TGGACCC	620	0.0	16.411291	5
GGACCCT	635	0.0	16.31496	6
ATGTGAC	80	3.3817298E-4	16.1875	11
GATCATC	85	5.362835E-4	15.235294	11
CAATGCG	85	5.362835E-4	15.235294	19
>>END_MODULE