FastQCFastQC Report
Wed 25 May 2016
SRR2088457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946806
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT172280.8849366603554745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140570.7220544830866558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT121060.621839053300637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59820.3072725274115654No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG33170.17038164049217025No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG33110.17007344337340238No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA31330.16093026218328893No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29130.1496297011618004No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA28420.14598270192304727No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG26550.13637722505478203No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG20420.10488975275399809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46950.022.5782741
TATACCG701.21974896E-418.55
CCGTTTA2950.017.55932227
CGTTTAC3100.016.70967928
CGCCGTT3300.015.6969725
TGTTACG2250.015.62222216
GTATTGG7750.015.0387111
ACCGTTA3600.014.9027788
CCGTTAT3600.014.9027789
GGACCGT4250.014.86
TAGCGAA2500.014.79999910
GACCGTT3800.014.6052647
GTATCAA72750.014.5202762
GTATTAG6650.014.4661651
GTATACG908.277651E-414.3888881
TGTCGGG2850.014.28070223
ACCGTAT800.0063006913.8750018
TGGACCC9750.013.6615385
ATAACGC950.001245616813.6315793
CATAGCG1501.3067147E-613.5666662
Produced by FastQC (version 0.11.5)