##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1946806
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.465701769976054	31.0	31.0	34.0	30.0	34.0
2	31.593550153430797	31.0	31.0	34.0	30.0	34.0
3	31.75184943954354	31.0	31.0	34.0	30.0	34.0
4	35.46722323641904	37.0	35.0	37.0	33.0	37.0
5	35.33109770567791	37.0	35.0	37.0	33.0	37.0
6	35.39534499071813	37.0	35.0	37.0	33.0	37.0
7	35.24474549595594	37.0	35.0	37.0	32.0	37.0
8	35.27000224983897	37.0	35.0	37.0	32.0	37.0
9	36.85074373101377	39.0	37.0	39.0	33.0	39.0
10	36.6790876954355	39.0	35.0	39.0	32.0	39.0
11	36.71105441425597	39.0	35.0	39.0	32.0	39.0
12	36.57299135096152	38.0	35.0	39.0	32.0	39.0
13	36.57524529922345	39.0	35.0	39.0	32.0	39.0
14	37.5868294015942	39.0	36.0	41.0	32.0	41.0
15	37.600837474304065	39.0	36.0	41.0	32.0	41.0
16	37.48139568092558	39.0	36.0	41.0	32.0	41.0
17	37.51747015367736	39.0	36.0	41.0	32.0	41.0
18	37.38953598869122	39.0	36.0	41.0	32.0	41.0
19	37.460479883460394	39.0	36.0	41.0	32.0	41.0
20	37.3573160345715	39.0	36.0	41.0	31.0	41.0
21	37.194298764232286	39.0	36.0	40.0	31.0	41.0
22	37.09208056683614	39.0	36.0	40.0	31.0	41.0
23	36.90486211774568	39.0	36.0	40.0	31.0	41.0
24	37.12185754512776	39.0	36.0	41.0	31.0	41.0
25	37.08375153970144	39.0	36.0	41.0	31.0	41.0
26	36.92033926338834	39.0	36.0	41.0	31.0	41.0
27	36.87069384417348	39.0	36.0	40.0	30.0	41.0
28	36.68383752669757	39.0	35.0	40.0	30.0	41.0
29	36.53566611156941	39.0	35.0	40.0	30.0	41.0
30	36.37820563528158	38.0	35.0	40.0	30.0	41.0
31	36.157999821245674	38.0	35.0	40.0	29.0	41.0
32	36.01991004753427	38.0	35.0	40.0	29.0	41.0
33	35.84356479279394	38.0	35.0	40.0	28.0	41.0
34	35.74635120294472	38.0	34.0	40.0	27.0	41.0
35	35.526520362069974	38.0	34.0	40.0	27.0	41.0
36	35.39227945671012	38.0	34.0	40.0	26.0	41.0
37	35.25008912033351	38.0	34.0	40.0	26.0	41.0
38	35.18856218852829	38.0	34.0	40.0	25.0	41.0
39	35.121547807023404	38.0	34.0	40.0	25.0	41.0
40	35.03414824075948	38.0	34.0	40.0	25.0	41.0
41	34.849975806526174	38.0	33.0	40.0	24.0	41.0
42	34.665108901451916	38.0	33.0	40.0	23.0	41.0
43	34.338054741972236	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	1.0
13	2.0
14	2.0
15	7.0
16	27.0
17	60.0
18	169.0
19	374.0
20	796.0
21	1649.0
22	2988.0
23	4955.0
24	8286.0
25	12337.0
26	18047.0
27	24830.0
28	33615.0
29	44190.0
30	56064.0
31	69585.0
32	85691.0
33	105775.0
34	129265.0
35	158875.0
36	201340.0
37	267565.0
38	356950.0
39	363358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59983069704942	20.95812320282555	13.102897772043029	24.339148328081997
2	19.23807508298207	23.000288677967912	34.73628086208898	23.02535537696103
3	19.216501284668325	24.932427781710146	31.973704621826727	23.877366311794805
4	13.8107238214799	16.528919676639582	36.769611353159995	32.89074514872052
5	12.753042676055035	38.280547727919476	35.328995287666054	13.637414308359435
6	32.41458060022416	38.07436385546377	14.016188567325147	15.49486697698692
7	26.750225754389493	32.22406341463916	21.8858478965033	19.139862934468045
8	26.862512237993926	34.5656423906645	19.05254041748382	19.519304953857755
9	25.220283890639333	14.061544909970486	20.92766305425399	39.79050814513619
10	16.44575782075872	27.297121541643083	33.791605326879	22.465515310719198
11	34.10581228946285	22.586431313649125	22.905209866828024	20.402546530060008
12	21.33761658840172	26.9947801681318	30.398046852125994	21.269556391340483
13	30.728588262004536	21.04878452192977	25.58164501239466	22.64098220367104
14	21.119669859246375	22.368330485934397	26.895386597329164	29.616613057490067
15	23.503728671475226	29.236760108608667	24.61688529827831	22.6426259216378
16	22.85127537104365	27.954043700296793	25.662084460393075	23.532596468266483
17	22.38137749729557	26.73913065811385	26.521492126077277	24.357999718513298
18	22.95005254760875	26.03479750935635	28.523591975779816	22.491557967255083
19	23.50768386783275	25.1281843183142	29.126014610598077	22.238117203254973
20	23.941060383006832	25.07363342829229	28.94212366306658	22.043182525634293
21	22.951850364134895	25.728449573301088	29.16068678645946	22.159013276104552
22	22.338383999227453	26.12823260252948	28.76306113706245	22.77032226118062
23	22.819890631115786	26.28875193522108	29.522664302452323	21.368693131210815
24	23.023454828061965	26.4291870890063	27.36127790853326	23.186080174398477
25	22.96212360142716	26.03315379138959	28.68739874440494	22.317323862778316
26	22.55530340465357	26.848797466208758	28.622163687599073	21.973735441538604
27	22.127885367109	26.72875468844867	28.002276549383964	23.141083395058367
28	22.244178413257405	26.525036392943107	28.52271875060997	22.708066443189512
29	22.884098364192425	26.5944834770388	27.998424085399364	22.522994073369407
30	22.48940058742371	26.61749552857347	29.077370832019216	21.815733051983607
31	22.770065430248316	26.45692482969541	29.151800436201658	21.62120930385462
32	22.29944842988978	26.27210929080761	28.40221367717174	23.026228602130875
33	22.128707226092377	26.268975953433472	29.008077846482905	22.594238973991246
34	21.906599835833667	26.438792565874564	29.410994213085434	22.24361338520633
35	21.62706504911121	27.892661107475526	28.42892409413162	22.05134974928164
36	23.210787310086367	26.38557719670065	28.18113361064225	22.222501882570732
37	22.206629730954187	26.55647249905743	28.41464429429537	22.822253475693007
38	22.057719156402847	25.682220005485906	29.49353967472876	22.76652116338248
39	21.586845325112	25.7680015368763	30.05954368334595	22.585609454665743
40	21.07708729066995	26.085804132512436	29.848531389362886	22.988577187454734
41	21.762106753318	25.011326244114716	30.172857490679604	23.053709511887675
42	20.745056261384033	26.575888917539807	29.68826888760359	22.990785933472573
43	21.278237276852444	25.797074798413398	29.335742749919614	23.588945174814544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	188.5
2	279.0
3	661.0
4	1043.0
5	1043.0
6	1643.5
7	2244.0
8	2558.5
9	2873.0
10	4262.0
11	5651.0
12	5651.0
13	10688.5
14	15726.0
15	25337.5
16	34949.0
17	32319.5
18	29690.0
19	29690.0
20	31928.5
21	34167.0
22	28721.5
23	23276.0
24	26465.5
25	29655.0
26	29655.0
27	33561.5
28	37468.0
29	42299.0
30	47130.0
31	53849.5
32	60569.0
33	60569.0
34	69794.0
35	79019.0
36	86615.0
37	94211.0
38	100923.0
39	107635.0
40	107635.0
41	111049.5
42	114464.0
43	122098.0
44	129732.0
45	130644.0
46	131556.0
47	131556.0
48	153691.5
49	175827.0
50	170484.5
51	165142.0
52	154712.0
53	144282.0
54	144282.0
55	131974.5
56	119667.0
57	105235.0
58	90803.0
59	82057.0
60	73311.0
61	73311.0
62	65728.0
63	58145.0
64	50944.0
65	43743.0
66	37703.5
67	31664.0
68	31664.0
69	27237.0
70	22810.0
71	19363.0
72	15916.0
73	13069.5
74	10223.0
75	10223.0
76	8210.0
77	6197.0
78	4871.0
79	3545.0
80	2736.0
81	1927.0
82	1927.0
83	1491.5
84	1056.0
85	812.5
86	569.0
87	420.0
88	271.0
89	271.0
90	222.0
91	173.0
92	113.0
93	53.0
94	42.0
95	31.0
96	31.0
97	22.0
98	13.0
99	8.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1946806.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.14337556139536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83788266592383	44.85122591891916
2	10.155777392504358	10.99736138960584
3	2.8713440145456985	4.663927720365373
4	1.2282965497959974	2.6601648558548336
5	0.6260576020254334	1.6948435934764818
6	0.4091861296125782	1.3292830974076575
7	0.2794661166058822	1.0591867235653893
8	0.21011741574959428	0.9101172922336105
9	0.16459980267456736	0.8020790040186604
>10	1.0235066533134622	10.859664378500046
>50	0.10734258163512814	4.005276095802172
>100	0.0749773329455881	8.030165896655804
>500	0.008393544623200827	3.1075931734445468
>1k	0.0026706732892002633	2.4793610013639
>5k	9.538118890000939E-5	0.3089260052105572
>10k+	2.861435667000282E-4	2.240823853575942
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17228	0.8849366603554745	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14057	0.7220544830866558	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12106	0.621839053300637	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5982	0.3072725274115654	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3317	0.17038164049217025	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3311	0.17007344337340238	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3133	0.16093026218328893	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2913	0.1496297011618004	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2842	0.14598270192304727	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2655	0.13637722505478203	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	2042	0.10488975275399809	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.13661864613115E-5	0.0	0.0	0.0	0.0
3	5.13661864613115E-5	0.0	0.0	0.0	0.0
4	5.13661864613115E-5	0.0	0.0	0.0	0.0
5	5.13661864613115E-5	0.0	0.0	0.0	0.0
6	5.13661864613115E-5	0.0	0.0	0.0	0.0
7	5.13661864613115E-5	0.0	0.0	0.0	0.0
8	5.13661864613115E-5	0.0	0.0	0.0	0.0
9	5.13661864613115E-5	0.0	0.0	1.02732372922623E-4	0.0
10	5.13661864613115E-5	0.0	0.0	2.05464745845246E-4	0.0
11	5.13661864613115E-5	0.0	0.0	2.05464745845246E-4	0.0
12	5.13661864613115E-5	0.0	0.0	4.10929491690492E-4	0.0
13	5.13661864613115E-5	0.0	0.0	4.6229567815180353E-4	0.0
14	5.13661864613115E-5	0.0	0.0	5.13661864613115E-4	0.0
15	5.13661864613115E-5	0.0	0.0	7.19126610458361E-4	0.0
16	5.13661864613115E-5	0.0	0.0	8.732251698422955E-4	0.0
17	5.13661864613115E-5	0.0	0.0	0.0012327884750714762	0.0
18	5.13661864613115E-5	0.0	0.0	0.0014896194073780336	0.0
19	5.13661864613115E-5	0.0	0.0	0.0019519150855298371	0.0
20	5.13661864613115E-5	0.0	0.0	0.0030306050012173786	0.0
21	5.13661864613115E-5	0.0	0.0	0.005085252459669839	0.0
22	5.13661864613115E-5	0.0	0.0	0.00852678695257771	0.0
23	5.13661864613115E-5	0.0	0.0	0.011711490513179022	0.0
24	5.13661864613115E-5	0.0	0.0	0.015512588311316074	0.0
25	5.13661864613115E-5	0.0	0.0	0.017824066702075092	0.0
26	5.13661864613115E-5	0.0	0.0	0.0245530371285069	0.0
27	5.13661864613115E-5	0.0	0.0	0.06831702799354429	0.0
28	5.13661864613115E-5	0.0	0.0	0.15892698091129778	0.0
29	5.13661864613115E-5	0.0	0.0	0.2761446184160106	0.0
30	5.13661864613115E-5	0.0	0.0	0.4290103893248737	0.0
31	5.13661864613115E-5	0.0	0.0	0.6193734763504941	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4695	0.0	22.578274	1
TATACCG	70	1.21974896E-4	18.5	5
CCGTTTA	295	0.0	17.559322	27
CGTTTAC	310	0.0	16.709679	28
CGCCGTT	330	0.0	15.69697	25
TGTTACG	225	0.0	15.622222	16
GTATTGG	775	0.0	15.038711	1
ACCGTTA	360	0.0	14.902778	8
CCGTTAT	360	0.0	14.902778	9
GGACCGT	425	0.0	14.8	6
TAGCGAA	250	0.0	14.799999	10
GACCGTT	380	0.0	14.605264	7
GTATCAA	7275	0.0	14.520276	2
GTATTAG	665	0.0	14.466165	1
GTATACG	90	8.277651E-4	14.388888	1
TGTCGGG	285	0.0	14.280702	23
ACCGTAT	80	0.00630069	13.875001	8
TGGACCC	975	0.0	13.661538	5
ATAACGC	95	0.0012456168	13.631579	3
CATAGCG	150	1.3067147E-6	13.566666	2
>>END_MODULE