FastQCFastQC Report
Wed 25 May 2016
SRR2088456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235369
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142491.1534205569348106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118480.9590656718761763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98530.7975754612589437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50150.40595158207790544No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG29690.24033305028699925No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG28550.23110503825172884No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA26670.215886913140932No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25100.2031781597239367No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA24890.20147826277007114No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23610.1911169860988903No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA15360.12433532005417004No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15120.12239258067832365No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14180.11478351812292521No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13410.10855056262541798No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13170.10660782324957159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC400.001931268123.1250023
GGTATCA37500.022.3973331
CTACGGA450.003825968120.55555525
GTCTAAT609.237347E-418.51
AATATAC1054.797985E-717.6190473
TTATGCG1401.869921E-917.1785724
TGTTACG1452.9795046E-916.58620616
TATACTG1352.2195309E-816.4444455
GTATTGG4750.015.9684211
TAGAGCT1557.208655E-915.5161294
CGTTATT1557.208655E-915.51612910
TTAACGG1455.345828E-815.31034535
ACCGTTA1853.0559022E-1015.08
CTAATAC2850.014.9298263
CGTTTAC1752.237357E-914.828
TCTATGC1001.0934686E-414.83
GCTAGGT750.004104638514.79999917
CGAACTA1904.5474735E-1014.60526424
GTATCAA57300.014.5610812
TCTAATA2800.014.5357142
Produced by FastQC (version 0.11.5)