##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1235369
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.497772730253065	31.0	31.0	34.0	30.0	34.0
2	31.61914618223381	31.0	31.0	34.0	30.0	34.0
3	31.77599486469225	31.0	31.0	34.0	30.0	34.0
4	35.47265230064863	37.0	35.0	37.0	33.0	37.0
5	35.34784991366952	37.0	35.0	37.0	33.0	37.0
6	35.42480910561945	37.0	35.0	37.0	33.0	37.0
7	35.26936000498637	37.0	35.0	37.0	32.0	37.0
8	35.309928450527735	37.0	35.0	37.0	32.0	37.0
9	36.87987475806824	39.0	37.0	39.0	33.0	39.0
10	36.72975119174919	39.0	35.0	39.0	32.0	39.0
11	36.75912460163725	39.0	35.0	39.0	32.0	39.0
12	36.62324050546841	39.0	35.0	39.0	32.0	39.0
13	36.63067957832842	39.0	35.0	39.0	32.0	39.0
14	37.61768912770192	39.0	36.0	41.0	32.0	41.0
15	37.6339385236314	39.0	36.0	41.0	32.0	41.0
16	37.51572283261115	39.0	36.0	41.0	32.0	41.0
17	37.57310730639995	39.0	36.0	41.0	32.0	41.0
18	37.45256599445186	39.0	36.0	41.0	32.0	41.0
19	37.532540479808056	39.0	36.0	41.0	32.0	41.0
20	37.431736590443826	39.0	36.0	41.0	32.0	41.0
21	37.28279647619456	39.0	36.0	41.0	31.0	41.0
22	37.18842710153808	39.0	36.0	40.0	31.0	41.0
23	36.989592583268646	39.0	36.0	40.0	31.0	41.0
24	37.19919068715501	39.0	36.0	41.0	31.0	41.0
25	37.1702114914653	39.0	36.0	41.0	31.0	41.0
26	36.99996600206092	39.0	36.0	41.0	31.0	41.0
27	36.928629421654584	39.0	36.0	41.0	31.0	41.0
28	36.73992628923018	39.0	35.0	40.0	30.0	41.0
29	36.58635355104426	39.0	35.0	40.0	30.0	41.0
30	36.41474085880413	38.0	35.0	40.0	30.0	41.0
31	36.15740236318056	38.0	35.0	40.0	30.0	41.0
32	36.01134964532864	38.0	35.0	40.0	29.0	41.0
33	35.812766873703325	38.0	35.0	40.0	28.0	41.0
34	35.71241062387028	38.0	34.0	40.0	28.0	41.0
35	35.47324321720878	38.0	34.0	40.0	27.0	41.0
36	35.31787263562547	38.0	34.0	40.0	26.0	41.0
37	35.1722707952037	38.0	34.0	40.0	25.0	41.0
38	35.08573956445402	38.0	34.0	40.0	25.0	41.0
39	34.98796715799085	38.0	34.0	40.0	24.0	41.0
40	34.86542077711194	38.0	33.0	40.0	24.0	41.0
41	34.6605054845961	38.0	33.0	40.0	23.0	41.0
42	34.436369214380484	38.0	33.0	40.0	23.0	41.0
43	34.09944882864958	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	2.0
16	17.0
17	37.0
18	106.0
19	239.0
20	497.0
21	1023.0
22	1911.0
23	3382.0
24	5079.0
25	7736.0
26	11154.0
27	15865.0
28	21254.0
29	27958.0
30	35596.0
31	43771.0
32	54255.0
33	66520.0
34	82682.0
35	100546.0
36	128878.0
37	172622.0
38	223625.0
39	230608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53795343739401	21.093292773252365	13.756294677946427	23.6124591114072
2	19.023223020814022	23.54009206965692	34.83218374429017	22.604501165238887
3	19.679140402584167	24.609327253638387	32.544850971653005	23.166681372124444
4	14.015650384621923	16.44002723073025	36.08897422551481	33.45534815913302
5	12.696692243370201	38.43669381375119	34.92810650097258	13.938507441906022
6	32.4412382049412	37.42833113021292	13.746580981067194	16.383849683778692
7	26.25231813328649	32.92295662267711	21.399759909792134	19.424965334244263
8	27.346485139257986	34.21512115003695	18.937094908484834	19.501298802220226
9	24.89790499842557	14.347130290625715	21.273400902888127	39.481563808060585
10	16.481877074784943	27.590541773348694	33.32065156240767	22.606929589458698
11	33.51557307978426	22.530434226534744	23.19849372940393	20.75549896427707
12	21.243774127406468	27.134240862446767	30.65731777307023	20.964667237076533
13	31.497552553123803	20.954629750301326	25.815606511091016	21.732211185483852
14	20.762784236936493	22.82532587429343	27.456249913993307	28.955639974776766
15	23.014581068490468	29.519924815986155	25.062956897898523	22.402537217624854
16	22.20275885180865	28.180082226444082	26.113897952757437	23.50326096898983
17	22.035683265485858	26.84987238630725	26.753302049832882	24.36114229837401
18	23.15138229953965	25.642540811692697	28.957097029308652	22.248979859458995
19	23.1052422393633	25.103835372265294	29.947246531198367	21.84367585717304
20	23.875376506938412	24.77284115110546	29.706832533437378	21.644949808518753
21	22.821440395541735	25.49732104334818	30.076681542114137	21.604557018995944
22	21.764590175081292	25.802088282934086	29.80858350824733	22.624738033737287
23	22.574712494809244	25.969973343996816	30.459563094103864	20.995751067090076
24	22.489231962272	26.132030186932003	28.561182934005952	22.817554916790044
25	22.46559529986587	26.188207733883562	29.428454170373385	21.91774279587718
26	22.14342435337134	26.900140767657277	29.39931307973569	21.55712179923569
27	21.33224971648147	26.905726143362834	29.13688136904844	22.625142771107257
28	21.706955573597845	26.511269102592017	29.260326266888683	22.521449056921455
29	22.020060403005093	26.550204837582942	28.690860787343702	22.738873972068266
30	21.84116648547924	26.745854882225473	30.166533238247034	21.246445394048255
31	22.403913324682748	26.448939547616945	30.026494108238104	21.120653019462203
32	22.054867816822345	26.390090734023598	29.01570300048002	22.53933844867404
33	21.75892385190174	26.333670344650063	29.659154471255146	22.248251332193053
34	21.129881031497472	26.751602152879016	30.125735711354256	21.992781104269252
35	20.865263739012395	28.464126912687625	29.17096025559974	21.49964909270024
36	22.701719081505203	26.408951495464105	29.059738426332537	21.829590996698155
37	21.77851314060819	27.06098339848256	29.07843729282506	22.08206616808419
38	21.6595203538376	25.787679632563226	30.32745681654631	22.22534319705286
39	21.077669910771597	25.69305203546471	31.00377296176284	22.22550509200085
40	20.304540586658725	26.161737909887655	30.738022404641853	22.795699098811774
41	21.318326750954572	25.192877593658253	30.861710144904077	22.627085510483102
42	20.18797622410794	26.836758895520283	30.356597907184007	22.618666973187768
43	20.45372678122893	26.55465694865259	29.890016667084897	23.101599603033588
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	147.0
2	231.0
3	523.0
4	815.0
5	815.0
6	1309.5
7	1804.0
8	2028.5
9	2253.0
10	3438.5
11	4624.0
12	4624.0
13	8826.0
14	13028.0
15	21025.0
16	29022.0
17	26477.0
18	23932.0
19	23932.0
20	25427.5
21	26923.0
22	21661.5
23	16400.0
24	18714.0
25	21028.0
26	21028.0
27	23416.5
28	25805.0
29	28340.5
30	30876.0
31	34042.0
32	37208.0
33	37208.0
34	41340.0
35	45472.0
36	50147.5
37	54823.0
38	58151.0
39	61479.0
40	61479.0
41	63776.0
42	66073.0
43	69742.0
44	73411.0
45	77762.0
46	82113.0
47	82113.0
48	102349.5
49	122586.0
50	115801.5
51	109017.0
52	101007.0
53	92997.0
54	92997.0
55	84499.0
56	76001.0
57	66436.0
58	56871.0
59	50862.0
60	44853.0
61	44853.0
62	39786.0
63	34719.0
64	30151.0
65	25583.0
66	22053.5
67	18524.0
68	18524.0
69	15953.0
70	13382.0
71	11405.5
72	9429.0
73	7733.0
74	6037.0
75	6037.0
76	4784.5
77	3532.0
78	2772.5
79	2013.0
80	1590.0
81	1167.0
82	1167.0
83	897.0
84	627.0
85	484.0
86	341.0
87	252.0
88	163.0
89	163.0
90	126.0
91	89.0
92	62.0
93	35.0
94	23.0
95	11.0
96	11.0
97	8.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1235369.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.80413513585192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.34612342911204	51.040510988757134
2	8.899912513562825	10.645031413167413
3	2.294052111417022	4.1158140743961
4	0.9909960128734219	2.3706263789189026
5	0.5414716599354918	1.61911221615081
6	0.34180376755594366	1.2264767222915383
7	0.2464287459026503	1.031622061492443
8	0.17491261653112922	0.8368398204794477
9	0.1348287687836268	0.7256986117682893
>10	0.8871938270749221	10.124083974501447
>50	0.0866456632313384	3.6142116768118506
>100	0.048710952952513605	5.297730555591621
>500	0.004206232609446167	1.7163020118589254
>1k	0.002170958766165764	2.3118265953317
>5k	2.713698457707205E-4	1.206466753927403
>10k+	2.713698457707205E-4	2.1176461445549797
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14249	1.1534205569348106	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11848	0.9590656718761763	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9853	0.7975754612589437	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5015	0.40595158207790544	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2969	0.24033305028699925	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2855	0.23110503825172884	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2667	0.215886913140932	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2510	0.2031781597239367	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2489	0.20147826277007114	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2361	0.1911169860988903	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1536	0.12433532005417004	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1512	0.12239258067832365	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1418	0.11478351812292521	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1341	0.10855056262541798	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1317	0.10660782324957159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.094747399360029E-5	0.0
5	0.0	0.0	0.0	8.094747399360029E-5	0.0
6	8.094747399360029E-5	0.0	0.0	8.094747399360029E-5	0.0
7	8.094747399360029E-5	0.0	0.0	8.094747399360029E-5	0.0
8	8.094747399360029E-5	0.0	0.0	8.094747399360029E-5	0.0
9	8.094747399360029E-5	0.0	0.0	2.428424219808009E-4	0.0
10	8.094747399360029E-5	0.0	0.0	2.428424219808009E-4	0.0
11	8.094747399360029E-5	0.0	0.0	4.0473736996800145E-4	0.0
12	1.6189494798720059E-4	0.0	0.0	5.666323179552021E-4	0.0
13	1.6189494798720059E-4	0.0	0.0	5.666323179552021E-4	0.0
14	1.6189494798720059E-4	0.0	0.0	6.475797919488023E-4	0.0
15	1.6189494798720059E-4	0.0	0.0	8.094747399360029E-4	0.0
16	2.428424219808009E-4	0.0	0.0	0.0012951595838976047	0.0
17	2.428424219808009E-4	0.0	0.0	0.0017808444278592064	0.0
18	2.428424219808009E-4	0.0	0.0	0.002428424219808009	0.0
19	2.428424219808009E-4	0.0	0.0	0.0031569514857504115	0.0
20	2.428424219808009E-4	0.0	0.0	0.004694953491628817	0.0
21	2.428424219808009E-4	0.0	0.0	0.00849948476932803	0.0
22	2.428424219808009E-4	0.0	0.0	0.014408650370860853	0.0
23	2.428424219808009E-4	0.0	0.0	0.018456024070540866	0.0
24	2.428424219808009E-4	0.0	0.0	0.022665292718208083	0.0
25	2.428424219808009E-4	0.0	0.0	0.026307929047920096	0.0
26	2.428424219808009E-4	0.0	0.0	0.03326941181136972	0.0
27	2.428424219808009E-4	0.0	0.0	0.09058022339883873	0.0
28	2.428424219808009E-4	0.0	0.0	0.19726899412240392	0.0
29	2.428424219808009E-4	0.0	0.0	0.3360129645474348	0.0
30	2.428424219808009E-4	0.0	0.0	0.5258347910624275	0.0
31	2.428424219808009E-4	0.0	0.0	0.7509497162386299	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	40	0.0019312681	23.125002	3
GGTATCA	3750	0.0	22.397333	1
CTACGGA	45	0.0038259681	20.555555	25
GTCTAAT	60	9.237347E-4	18.5	1
AATATAC	105	4.797985E-7	17.619047	3
TTATGCG	140	1.869921E-9	17.178572	4
TGTTACG	145	2.9795046E-9	16.586206	16
TATACTG	135	2.2195309E-8	16.444445	5
GTATTGG	475	0.0	15.968421	1
TAGAGCT	155	7.208655E-9	15.516129	4
CGTTATT	155	7.208655E-9	15.516129	10
TTAACGG	145	5.345828E-8	15.310345	35
ACCGTTA	185	3.0559022E-10	15.0	8
CTAATAC	285	0.0	14.929826	3
CGTTTAC	175	2.237357E-9	14.8	28
TCTATGC	100	1.0934686E-4	14.8	3
GCTAGGT	75	0.0041046385	14.799999	17
CGAACTA	190	4.5474735E-10	14.605264	24
GTATCAA	5730	0.0	14.561081	2
TCTAATA	280	0.0	14.535714	2
>>END_MODULE