FastQCFastQC Report
Wed 25 May 2016
SRR2088455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222594
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140311.1476418173162963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115650.94593953511959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98400.804846089544035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50670.4144466601341083No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG27980.22885765838863925No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG27690.22648565263693426No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26300.21511638368910693No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25810.21110851190174335No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA24350.1991666898414355No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG22210.18166292325988842No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.12637065125462746No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA14830.12129946654408578No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14550.11900925409416371No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13760.11254758325331223No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC13160.10763998514633641No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.10134190090905076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.022.5056321
GTATAAC603.7265367E-521.5833321
CGTATAC656.90259E-519.9230773
GTATTGG5800.018.8189661
CTCTTAG801.616807E-518.51
CCGTATA609.237275E-418.52
CTAATAC2600.017.0769233
TGTTACG1005.8784044E-616.65000216
TCTAATA2700.016.4444452
GACCGTT2150.016.3488377
TAATACT2850.016.228074
ACCGTTA2100.015.8571448
TTGGACC7450.015.3959734
ATTGGAC6400.015.3203133
CGCCGTT1954.1836756E-1115.17948625
CGTTATT1101.451939E-515.13636410
ATACCGT750.004104603514.86
TTATACC1152.2099615E-514.478264
CCGTTTA2201.8189894E-1114.29545427
TCTTAGA1304.4442295E-614.2307692
Produced by FastQC (version 0.11.5)