##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222594
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.466187466975953	31.0	31.0	34.0	30.0	34.0
2	31.581717234012274	31.0	31.0	34.0	30.0	34.0
3	31.7428892993095	31.0	31.0	34.0	30.0	34.0
4	35.44700530184182	37.0	35.0	37.0	33.0	37.0
5	35.32302219706624	37.0	35.0	37.0	33.0	37.0
6	35.394195456545674	37.0	35.0	37.0	33.0	37.0
7	35.242944100821695	37.0	35.0	37.0	32.0	37.0
8	35.28113748308923	37.0	35.0	37.0	32.0	37.0
9	36.83929088479086	39.0	37.0	39.0	33.0	39.0
10	36.68876830738577	39.0	35.0	39.0	32.0	39.0
11	36.72305851329223	39.0	35.0	39.0	32.0	39.0
12	36.57941393463406	38.0	35.0	39.0	32.0	39.0
13	36.592041184563314	39.0	35.0	39.0	32.0	39.0
14	37.5586016289954	39.0	36.0	41.0	32.0	41.0
15	37.58825088295869	39.0	36.0	41.0	32.0	41.0
16	37.464967110913356	39.0	36.0	41.0	32.0	41.0
17	37.527271522680465	39.0	36.0	41.0	32.0	41.0
18	37.40375300385901	39.0	36.0	41.0	32.0	41.0
19	37.48209790003877	39.0	36.0	41.0	32.0	41.0
20	37.38542885046058	39.0	36.0	41.0	32.0	41.0
21	37.22999458528342	39.0	36.0	41.0	31.0	41.0
22	37.135390816575246	39.0	36.0	40.0	31.0	41.0
23	36.92823864668075	39.0	36.0	40.0	31.0	41.0
24	37.13374022774527	39.0	36.0	41.0	31.0	41.0
25	37.09737737957163	39.0	36.0	41.0	31.0	41.0
26	36.929444279949024	39.0	36.0	41.0	31.0	41.0
27	36.8659236017844	39.0	35.0	40.0	30.0	41.0
28	36.675519428362975	39.0	35.0	40.0	30.0	41.0
29	36.5077114724921	39.0	35.0	40.0	30.0	41.0
30	36.33037950456161	38.0	35.0	40.0	30.0	41.0
31	36.08075779858236	38.0	35.0	40.0	29.0	41.0
32	35.9394762284127	38.0	35.0	40.0	29.0	41.0
33	35.730946659316174	38.0	34.0	40.0	28.0	41.0
34	35.618349182148776	38.0	34.0	40.0	27.0	41.0
35	35.38598586284572	38.0	34.0	40.0	26.0	41.0
36	35.22686762735626	38.0	34.0	40.0	26.0	41.0
37	35.066699983804924	38.0	34.0	40.0	25.0	41.0
38	34.97924822140465	38.0	33.0	40.0	25.0	41.0
39	34.88095393892004	38.0	33.0	40.0	24.0	41.0
40	34.78025738716205	38.0	33.0	40.0	24.0	41.0
41	34.5601385251359	38.0	33.0	40.0	23.0	41.0
42	34.32375261125116	38.0	33.0	40.0	22.0	41.0
43	33.98782588496263	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	9.0
16	19.0
17	49.0
18	109.0
19	224.0
20	496.0
21	1039.0
22	1918.0
23	3246.0
24	5264.0
25	8074.0
26	11508.0
27	16161.0
28	21723.0
29	28377.0
30	36151.0
31	44564.0
32	54678.0
33	67400.0
34	82200.0
35	100330.0
36	127907.0
37	171436.0
38	219048.0
39	220660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69871600874861	21.21121157146199	13.760168952244161	23.329903467545236
2	19.046224666569607	23.62305066113526	34.85531582847617	22.47540884381896
3	19.648223367691973	24.89051966556355	32.53827517556932	22.922981791175157
4	13.990008130254198	16.500980701688377	36.27148505554583	33.237526112511595
5	12.641481963759023	38.517692709108665	34.935227884318095	13.905597442814214
6	32.422210480339345	37.41569155418725	13.68107482942007	16.48102313605334
7	26.13017894738564	32.87248260665437	21.564804015069598	19.432534430890385
8	27.196845395936837	34.44062378843672	18.94316510632311	19.419365709303335
9	24.911131577612846	14.41713275216466	21.502722899016355	39.16901277120614
10	16.62391603426812	27.5118314011029	33.20268216595206	22.66157039867691
11	33.40610210748621	22.66901358913916	23.080024930598384	20.844859372776245
12	21.08222353455031	27.31094705192402	30.72728968079346	20.87953973273221
13	31.51569531667913	21.076825176632635	25.888725120522434	21.51875438616581
14	20.73705580102634	22.96355126885949	27.427747886870048	28.871645043244122
15	23.053278520915367	29.563698169629493	25.0733276950484	22.309695614406742
16	22.113718863334846	28.327719586387634	26.17622857628943	23.382332973988092
17	21.83562163727288	26.910159873187666	26.805219067000163	24.448999422539288
18	23.140960940426666	25.67688046890464	29.0006330801558	22.18152551051289
19	23.166235070677594	25.084124410883746	29.91524578069253	21.834394737746138
20	23.996109910567203	24.753679471680705	29.71706061047249	21.533150007279602
21	22.976801783748325	25.430600837236238	30.08243128953684	21.5101660894786
22	21.75971745321832	25.825826071451356	29.87762086187238	22.536835613457942
23	22.493076197004076	26.160769642252458	30.558877272422407	20.787276888321063
24	22.570452660490727	26.132550953137347	28.54120010404108	22.75579628233085
25	22.388953323834404	26.28525904756608	29.42980253461084	21.89598509398868
26	22.270762002758072	26.890284100854412	29.356106769704414	21.4828471266831
27	21.294149979469882	27.043319368490277	29.076619057512143	22.5859115945277
28	21.686676034726165	26.684573946870344	29.199309010186536	22.429441008216955
29	21.893449501633413	26.551741624774866	28.8242867215118	22.73052215207992
30	21.8422468947173	26.795976423898693	30.082349496235057	21.279427185148954
31	22.42126167803866	26.517143058120684	30.057484332493047	21.00411093134761
32	21.937045331483716	26.489496922118054	29.154486280809493	22.41897146558874
33	21.652404641279116	26.476082820625653	29.806051722812317	22.065460815282915
34	21.1277824036434	26.777818310902884	30.189171548363564	21.905227737090154
35	20.7794247313499	28.52549579009876	29.292798754124426	21.402280724426916
36	22.700667596929154	26.600326846033923	29.025252863992463	21.673752693044463
37	21.629257136874546	27.180650322183812	29.11473473614299	22.07535780479865
38	21.568811886856963	25.863941750082205	30.35161304570446	22.215633317356374
39	21.07551648380411	25.78942805215795	30.962854389928303	22.17220107410964
40	20.207362378680084	26.213526321902446	30.7390679162502	22.840043383167266
41	21.299548337387556	25.29973155438355	30.842536443005606	22.55818366522329
42	20.09595990165173	26.861247478721474	30.500558648251175	22.542233971375616
43	20.342239533320136	26.650138966819732	29.849811139266187	23.15781036059395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	151.0
2	228.0
3	531.5
4	835.0
5	835.0
6	1308.5
7	1782.0
8	2078.5
9	2375.0
10	3565.5
11	4756.0
12	4756.0
13	8898.0
14	13040.0
15	20909.5
16	28779.0
17	26522.0
18	24265.0
19	24265.0
20	25699.0
21	27133.0
22	22251.0
23	17369.0
24	19666.5
25	21964.0
26	21964.0
27	24126.5
28	26289.0
29	28583.5
30	30878.0
31	33958.0
32	37038.0
33	37038.0
34	40834.5
35	44631.0
36	48966.0
37	53301.0
38	56659.0
39	60017.0
40	60017.0
41	61986.0
42	63955.0
43	68194.0
44	72433.0
45	76062.5
46	79692.0
47	79692.0
48	100199.0
49	120706.0
50	114648.5
51	108591.0
52	101334.5
53	94078.0
54	94078.0
55	84501.0
56	74924.0
57	65632.5
58	56341.0
59	50343.5
60	44346.0
61	44346.0
62	39382.0
63	34418.0
64	29894.0
65	25370.0
66	21771.0
67	18172.0
68	18172.0
69	15620.5
70	13069.0
71	10906.5
72	8744.0
73	7126.5
74	5509.0
75	5509.0
76	4429.5
77	3350.0
78	2645.5
79	1941.0
80	1492.5
81	1044.0
82	1044.0
83	812.5
84	581.0
85	422.5
86	264.0
87	205.0
88	146.0
89	146.0
90	126.5
91	107.0
92	71.0
93	35.0
94	26.5
95	18.0
96	18.0
97	11.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1222594.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.1717864600501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.69501370152776	51.56422065139395
2	8.67547280607959	10.440373942547854
3	2.2371003595386654	4.038309753715857
4	0.9317929561115077	2.242705871204819
5	0.534641188899338	1.6085157725599135
6	0.35194648826511143	1.270634936235169
7	0.24465476800345168	1.0304920119715755
8	0.17115070490146136	0.8238754934254227
9	0.13479772297129985	0.7299917821737041
>10	0.8824172932147242	10.181715532267031
>50	0.08679437447818267	3.6128282456407104
>100	0.04752579306709118	5.2040159263267185
>500	0.003960478475795343	1.594945050005055
>1k	0.002185091572852603	2.3290191461303142
>5k	2.731364466065754E-4	1.2249907702831344
>10k+	2.731364466065754E-4	2.1033651141186764
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14031	1.1476418173162963	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11565	0.94593953511959	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9840	0.804846089544035	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5067	0.4144466601341083	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2798	0.22885765838863925	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2769	0.22648565263693426	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2630	0.21511638368910693	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2581	0.21110851190174335	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2435	0.1991666898414355	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2221	0.18166292325988842	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1545	0.12637065125462746	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1483	0.12129946654408578	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1455	0.11900925409416371	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1376	0.11254758325331223	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1316	0.10763998514633641	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1239	0.10134190090905076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.179330178293039E-5	0.0
4	0.0	0.0	0.0	8.179330178293039E-5	0.0
5	0.0	0.0	0.0	8.179330178293039E-5	0.0
6	8.179330178293039E-5	0.0	0.0	8.179330178293039E-5	0.0
7	8.179330178293039E-5	0.0	0.0	8.179330178293039E-5	0.0
8	8.179330178293039E-5	0.0	0.0	8.179330178293039E-5	0.0
9	8.179330178293039E-5	0.0	0.0	3.2717320713172154E-4	0.0
10	8.179330178293039E-5	0.0	0.0	4.907598106975823E-4	0.0
11	8.179330178293039E-5	0.0	0.0	5.725531124805127E-4	0.0
12	8.179330178293039E-5	0.0	0.0	8.997263196122344E-4	0.0
13	8.179330178293039E-5	0.0	0.0	8.997263196122344E-4	0.0
14	8.179330178293039E-5	0.0	0.0	0.0012268995267439558	0.0
15	8.179330178293039E-5	0.0	0.0	0.0013904861303098166	0.0
16	8.179330178293039E-5	0.0	0.0	0.0017176593374415383	0.0
17	8.179330178293039E-5	0.0	0.0	0.002372005751704981	0.0
18	1.6358660356586077E-4	0.0	0.0	0.0026991789588367027	0.0
19	1.6358660356586077E-4	0.0	0.0	0.0039260784855806585	0.0
20	1.6358660356586077E-4	0.0	0.0	0.006052704331936849	0.0
21	1.6358660356586077E-4	0.0	0.0	0.011369268947827325	0.0
22	1.6358660356586077E-4	0.0	0.0	0.01873066610829106	0.0
23	1.6358660356586077E-4	0.0	0.0	0.023474677611701023	0.0
24	3.2717320713172154E-4	0.0	0.0	0.030508901565033036	0.0
25	3.2717320713172154E-4	0.0	0.0	0.03541649967200886	0.0
26	3.2717320713172154E-4	0.0	0.0	0.045068109282394644	0.0
27	3.2717320713172154E-4	0.0	0.0	0.10297776694470936	0.0
28	3.2717320713172154E-4	0.0	0.0	0.21806094255329242	0.0
29	3.2717320713172154E-4	0.0	0.0	0.3643891594429549	0.0
30	4.08966508914652E-4	0.0	0.0	0.5570941784435389	0.0
31	4.08966508914652E-4	0.0	0.0	0.7817803784412487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3995	0.0	22.505632	1
GTATAAC	60	3.7265367E-5	21.583332	1
CGTATAC	65	6.90259E-5	19.923077	3
GTATTGG	580	0.0	18.818966	1
CTCTTAG	80	1.616807E-5	18.5	1
CCGTATA	60	9.237275E-4	18.5	2
CTAATAC	260	0.0	17.076923	3
TGTTACG	100	5.8784044E-6	16.650002	16
TCTAATA	270	0.0	16.444445	2
GACCGTT	215	0.0	16.348837	7
TAATACT	285	0.0	16.22807	4
ACCGTTA	210	0.0	15.857144	8
TTGGACC	745	0.0	15.395973	4
ATTGGAC	640	0.0	15.320313	3
CGCCGTT	195	4.1836756E-11	15.179486	25
CGTTATT	110	1.451939E-5	15.136364	10
ATACCGT	75	0.0041046035	14.8	6
TTATACC	115	2.2099615E-5	14.47826	4
CCGTTTA	220	1.8189894E-11	14.295454	27
TCTTAGA	130	4.4442295E-6	14.230769	2
>>END_MODULE