FastQCFastQC Report
Wed 25 May 2016
SRR2088454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924740
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118890.6176938183858599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106730.5545164541704334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88120.4578280702848177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46730.24278603863378947No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG26580.13809657408273324No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA23030.11965252449681515No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22810.1185095129731808No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG22700.1179380072113636No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA20550.10676766732130054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33550.023.3248881
TGCGGTA1850.020.00000236
GTATTGG6300.017.3253971
TTCGCTA1005.881937E-616.65000226
GTCTAGA904.4484634E-516.4444451
ATTAGAG5350.016.2523373
TCGCTAC1151.2431847E-616.08695627
TAATACT7450.015.6442954
TTAACGG3700.015.50000135
GTATTAG6400.015.031251
TCGGGTA1601.0975782E-815.0312525
CTCTAAT5250.014.8000011
GTATAGA2006.184564E-1114.81
CCGGATT750.004105744414.79999931
CCGTATA750.004105744414.7999992
TATTAGA5800.014.6724142
TAGCGAA2151.2732926E-1114.62790710
TTAAGAC1651.646913E-814.5757573
TGGACCC8000.014.568755
ATACTGG6800.014.4191186
Produced by FastQC (version 0.11.5)