FastQCFastQC Report
Wed 25 May 2016
SRR2088453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987318
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115191.166696039168738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96470.9770914740742092No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82710.8377240159705384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40450.40969576164923566No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG25570.25898444067666143No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG24630.2494636986259746No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA23690.2399429565752878No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22560.22849780921648344No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA21300.2157359634889671No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG19400.19649191040779163No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA13570.1374430527955532No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13010.13177112136110147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.12903644013377655No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11520.11667973236586388No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC10930.1107039474617094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.022.8395061
GACCGTG603.7256028E-521.5833327
GTTATCG450.003825431220.55555711
AGTGTCG450.003825431220.5555578
CGCAATA609.235561E-418.528
AGTACCG1103.849891E-818.55
ACCGTTA1950.018.0256428
CGCCGTT1900.017.52631625
TAATACT2050.017.1463434
ATACTGA1301.3922545E-817.0769236
GGGTTAG650.001579720517.0769237
TAGGCGA650.001579720517.0769234
CTAATAC1950.017.0769233
CCGTTTA1851.8189894E-1217.027
GGACCGT2400.016.9583326
GTATTGG3950.016.860761
ATAGAAC1107.804865E-716.8181823
GACCGTT2100.016.7380947
ATAGTAC1554.0017767E-1016.7096793
TCGTATA904.444934E-516.44444530
Produced by FastQC (version 0.11.5)