##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	987318
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46346567164784	31.0	31.0	34.0	30.0	34.0
2	31.588184353977137	31.0	31.0	34.0	30.0	34.0
3	31.74435997317987	31.0	31.0	34.0	30.0	34.0
4	35.44722065231263	37.0	35.0	37.0	33.0	37.0
5	35.32861145041415	37.0	35.0	37.0	33.0	37.0
6	35.40302010091987	37.0	35.0	37.0	33.0	37.0
7	35.238831865721075	37.0	35.0	37.0	32.0	37.0
8	35.28451826058068	37.0	35.0	37.0	32.0	37.0
9	36.84314982609453	39.0	37.0	39.0	33.0	39.0
10	36.6918824532724	39.0	35.0	39.0	32.0	39.0
11	36.72783946003213	39.0	35.0	39.0	32.0	39.0
12	36.577912081011384	38.0	35.0	39.0	32.0	39.0
13	36.58489767227985	39.0	35.0	39.0	32.0	39.0
14	37.55335666927981	39.0	36.0	41.0	32.0	41.0
15	37.57150988840475	39.0	36.0	41.0	32.0	41.0
16	37.445549458229266	39.0	36.0	41.0	32.0	41.0
17	37.51240329863327	39.0	36.0	41.0	32.0	41.0
18	37.38971638317138	39.0	36.0	41.0	32.0	41.0
19	37.46684350938603	39.0	36.0	41.0	32.0	41.0
20	37.37152568878518	39.0	36.0	41.0	32.0	41.0
21	37.209122086298436	39.0	36.0	41.0	31.0	41.0
22	37.12089114145594	39.0	36.0	40.0	31.0	41.0
23	36.90837197336623	39.0	36.0	40.0	31.0	41.0
24	37.11342748739514	39.0	36.0	41.0	31.0	41.0
25	37.08509720272496	39.0	36.0	41.0	31.0	41.0
26	36.90342929025906	39.0	36.0	41.0	31.0	41.0
27	36.84248742553058	39.0	35.0	40.0	30.0	41.0
28	36.652827153966605	39.0	35.0	40.0	30.0	41.0
29	36.48951097822586	39.0	35.0	40.0	30.0	41.0
30	36.30352530795549	38.0	35.0	40.0	30.0	41.0
31	36.04173731259837	38.0	35.0	40.0	29.0	41.0
32	35.89467831033163	38.0	35.0	40.0	29.0	41.0
33	35.698342378038284	38.0	34.0	40.0	27.0	41.0
34	35.58461610139793	38.0	34.0	40.0	27.0	41.0
35	35.32464818832433	38.0	34.0	40.0	26.0	41.0
36	35.17697337635899	38.0	34.0	40.0	25.0	41.0
37	35.01541549936292	38.0	33.0	40.0	25.0	41.0
38	34.92818423243575	38.0	33.0	40.0	25.0	41.0
39	34.830641191591766	38.0	33.0	40.0	24.0	41.0
40	34.71116398161484	38.0	33.0	40.0	24.0	41.0
41	34.50931310884639	38.0	33.0	40.0	23.0	41.0
42	34.2626256180886	38.0	33.0	40.0	22.0	41.0
43	33.92924974526951	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	2.0
15	4.0
16	15.0
17	28.0
18	97.0
19	226.0
20	455.0
21	865.0
22	1647.0
23	2699.0
24	4201.0
25	6516.0
26	9546.0
27	13327.0
28	17863.0
29	23300.0
30	29241.0
31	36443.0
32	43971.0
33	54299.0
34	66361.0
35	81155.0
36	103460.0
37	138721.0
38	176999.0
39	175873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7852201620957	20.926287173939905	13.64778116067974	23.640711503284656
2	19.276666686923566	23.26687045106035	34.73298369927419	22.723479162741892
3	19.63713818648095	24.582353405893542	32.4303821058666	23.350126301758905
4	13.922869835250648	16.32270453896313	35.967945484636154	33.786480141150065
5	12.596954577957659	38.49073955908836	35.00280558037026	13.909500282583725
6	32.85354870467266	37.138287765441326	13.441363370261659	16.566800159624357
7	26.552539303446306	32.65958890651239	21.213023564849422	19.574848225191886
8	27.443437676614828	34.18736415217792	18.614266122971525	19.754932048235727
9	24.904032945818873	14.146100850992285	21.17240848439915	39.77745771878969
10	16.6486380274643	27.371930826744777	33.157300889885526	22.822130255905392
11	33.802989513003915	22.289171270046733	23.01619133855556	20.89164787839379
12	21.476363238591823	27.024423741894708	30.452498587081365	21.046714432432104
13	31.630538489119008	20.814165243619584	25.783081033668992	21.77221523359242
14	20.921830656384266	22.627360181825917	27.232259515171403	29.218549646618413
15	23.176423401578823	29.353460587166445	24.945053164228746	22.525062847025985
16	22.42509505549377	27.9780172143119	25.953542830172243	23.643344900022083
17	22.27144648431407	26.50179577400594	26.519723128718407	24.70703461296158
18	23.427203798573508	25.40458089490924	28.841163637247575	22.32705166926968
19	23.264439623302724	24.63583161656123	29.93898622328368	22.16074253685236
20	24.1168498903089	24.326103646444206	29.776829754952306	21.780216708294592
21	23.04141117654089	25.124529280333185	30.104181226312086	21.72987831681383
22	21.930522891307564	25.636015954332848	29.52179540938178	22.911665744977807
23	22.669190676154997	25.861171375382604	30.50121642672371	20.96842152173869
24	22.8122043758951	25.883251394181006	28.149998278163675	23.154545951760223
25	22.632930828770466	25.85843669415528	29.321859826317358	22.1867726507569
26	22.40159705383676	26.57806299490134	29.336546077352992	21.683793873908915
27	21.520219422718924	26.61634853208389	28.8222234376361	23.04120860756109
28	21.960300531338433	26.18538302755546	29.131546269793525	22.722770171312586
29	22.31074486639563	26.240177936591856	28.5038862858775	22.945190911135015
30	22.06725695267381	26.367289971417517	30.10063626916556	21.464816806743116
31	22.71456612763061	26.153984835686174	29.87274616688848	21.25870286979474
32	22.26152060430378	25.930855104434436	28.957438231653832	22.85018605960795
33	21.981468989727727	26.00104525593578	29.621763200913993	22.3957225534225
34	21.475350393692814	26.405676793089967	29.984564243739097	22.134408569478122
35	20.961534176425427	28.45486459276545	29.03836453908467	21.545236691724448
36	23.089318740264027	26.17333017325725	28.81888104946937	21.918470037009353
37	22.022894346097203	26.86469810132095	28.841163637247575	22.271243915334267
38	21.769480552365096	25.616062909822368	30.154823471262553	22.459633066549987
39	21.397158767489298	25.394148592449444	30.813071371128654	22.395621268932604
40	20.507475808199587	26.015731506971413	30.486023753238573	22.99076893159043
41	21.702531504540584	24.934114439319448	30.600272657846812	22.763081398293153
42	20.559637320498563	26.680968036640678	30.17680220557105	22.58259243728971
43	20.82905406363502	26.368100247336724	29.396405210884435	23.40644047814382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	120.5
2	177.0
3	398.5
4	620.0
5	620.0
6	1001.5
7	1383.0
8	1576.5
9	1770.0
10	2679.0
11	3588.0
12	3588.0
13	6883.5
14	10179.0
15	16509.0
16	22839.0
17	21034.0
18	19229.0
19	19229.0
20	20071.5
21	20914.0
22	16673.0
23	12432.0
24	14020.0
25	15608.0
26	15608.0
27	17362.0
28	19116.0
29	20847.0
30	22578.0
31	25170.0
32	27762.0
33	27762.0
34	31137.5
35	34513.0
36	38169.0
37	41825.0
38	45145.0
39	48465.0
40	48465.0
41	49913.0
42	51361.0
43	55043.0
44	58725.0
45	62024.0
46	65323.0
47	65323.0
48	82717.0
49	100111.0
50	94729.0
51	89347.0
52	83031.0
53	76715.0
54	76715.0
55	69481.5
56	62248.0
57	54236.5
58	46225.0
59	41745.5
60	37266.0
61	37266.0
62	33135.5
63	29005.0
64	25489.5
65	21974.0
66	18863.0
67	15752.0
68	15752.0
69	13434.0
70	11116.0
71	9375.5
72	7635.0
73	6286.5
74	4938.0
75	4938.0
76	3906.0
77	2874.0
78	2266.5
79	1659.0
80	1305.5
81	952.0
82	952.0
83	719.5
84	487.0
85	370.5
86	254.0
87	192.0
88	130.0
89	130.0
90	114.5
91	99.0
92	68.0
93	37.0
94	26.5
95	16.0
96	16.0
97	11.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	987318.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.333569335406956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.51842513442013	49.88594982033506
2	8.442851903778747	9.850033738353005
3	2.3258339357618603	4.070225854632207
4	1.0251589078944283	2.3920471293387884
5	0.5666335126037465	1.652687764761792
6	0.3690629081086184	1.2917254043568611
7	0.26551034990096906	1.084171648365144
8	0.19897113522106163	0.9285357209729946
9	0.1576576034726596	0.8277057669083838
>10	0.9738460949720464	10.78400504083085
>50	0.09311017665327256	3.7819967065292475
>100	0.05526718272324492	6.019599714987575
>500	0.005055988186138384	2.0031092626647746
>1k	0.002092133042540021	2.4344245745535495
>5k	3.486888404233368E-4	1.8222843099323434
>10k+	1.743444202116684E-4	1.1714975424774339
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11519	1.166696039168738	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9647	0.9770914740742092	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8271	0.8377240159705384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4045	0.40969576164923566	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2557	0.25898444067666143	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2463	0.2494636986259746	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2369	0.2399429565752878	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2256	0.22849780921648344	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2130	0.2157359634889671	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1940	0.19649191040779163	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1357	0.1374430527955532	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1301	0.13177112136110147	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1274	0.12903644013377655	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1152	0.11667973236586388	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1093	0.1107039474617094	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.0256897980184703E-4	0.0
10	1.0128448990092351E-4	0.0	0.0	3.038534697027705E-4	0.0
11	1.0128448990092351E-4	0.0	0.0	5.064224495046175E-4	0.0
12	1.0128448990092351E-4	0.0	0.0	7.089914293064646E-4	0.0
13	1.0128448990092351E-4	0.0	0.0	7.089914293064646E-4	0.0
14	1.0128448990092351E-4	0.0	0.0	7.089914293064646E-4	0.0
15	1.0128448990092351E-4	0.0	0.0	9.115604091083116E-4	0.0
16	1.0128448990092351E-4	0.0	0.0	0.0014179828586129292	0.0
17	1.0128448990092351E-4	0.0	0.0	0.0019244053081175468	0.0
18	1.0128448990092351E-4	0.0	0.0	0.002430827757622164	0.0
19	1.0128448990092351E-4	0.0	0.0	0.0030385346970277054	0.0
20	1.0128448990092351E-4	0.0	0.0	0.004962940005145252	0.0
21	1.0128448990092351E-4	0.0	0.0	0.008507897151677575	1.0128448990092351E-4
22	2.0256897980184703E-4	0.0	0.0	0.014382397565931139	1.0128448990092351E-4
23	2.0256897980184703E-4	0.0	0.0	0.017724785732661615	1.0128448990092351E-4
24	2.0256897980184703E-4	0.0	0.0	0.024308277576221643	1.0128448990092351E-4
25	2.0256897980184703E-4	0.0	0.0	0.029575071051069664	1.0128448990092351E-4
26	2.0256897980184703E-4	0.0	0.0	0.038386821672450014	1.0128448990092351E-4
27	2.0256897980184703E-4	0.0	0.0	0.08973805805221823	1.0128448990092351E-4
28	2.0256897980184703E-4	0.0	0.0	0.1900097030541325	1.0128448990092351E-4
29	2.0256897980184703E-4	0.0	0.0	0.32552835054156815	1.0128448990092351E-4
30	2.0256897980184703E-4	0.0	0.0	0.5031813458277881	1.0128448990092351E-4
31	2.0256897980184703E-4	0.0	0.0	0.7129415244126006	1.0128448990092351E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3240	0.0	22.839506	1
GACCGTG	60	3.7256028E-5	21.583332	7
GTTATCG	45	0.0038254312	20.555557	11
AGTGTCG	45	0.0038254312	20.555557	8
CGCAATA	60	9.235561E-4	18.5	28
AGTACCG	110	3.849891E-8	18.5	5
ACCGTTA	195	0.0	18.025642	8
CGCCGTT	190	0.0	17.526316	25
TAATACT	205	0.0	17.146343	4
ATACTGA	130	1.3922545E-8	17.076923	6
GGGTTAG	65	0.0015797205	17.076923	7
TAGGCGA	65	0.0015797205	17.076923	4
CTAATAC	195	0.0	17.076923	3
CCGTTTA	185	1.8189894E-12	17.0	27
GGACCGT	240	0.0	16.958332	6
GTATTGG	395	0.0	16.86076	1
ATAGAAC	110	7.804865E-7	16.818182	3
GACCGTT	210	0.0	16.738094	7
ATAGTAC	155	4.0017767E-10	16.709679	3
TCGTATA	90	4.444934E-5	16.444445	30
>>END_MODULE