FastQCFastQC Report
Wed 25 May 2016
SRR2088452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290241
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT186561.4459314190139672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT151521.1743542485473646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130541.011748967828491No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64470.49967409189446005No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG45040.3490820707139209No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41890.32466802713601567No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG39120.30319916976750855No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37820.2931235327353572No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA35470.2749098811772374No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG32970.2555336561154079No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA22290.17275842265127211No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA22240.17237089815003553No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.15702492790106654No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19950.1546222759933997No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19720.15283966328771137No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16070.12455037469744025No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG15820.1226127521912573No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.1171099042736977No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14990.11617984547072989No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC14880.11532729156800939No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14670.11369968866281571No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG13660.10587169373783659No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC13160.10199644872547066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54500.022.8110081
GTATTGG7150.020.69931
CAATGCG555.143565E-420.18181819
ATTGGAC8350.019.0538923
TATTGGA7800.018.737182
TGCGACG609.237654E-418.522
TATACTG1750.017.971435
TAGGGGT1258.580173E-917.764
TTGGACC9900.017.5656574
TGGACCC9300.017.5053775
ACCGTTA2700.017.129638
CCGTTTA2700.016.44444527
GGACCCT9800.016.423476
TGTTACG1606.2937033E-1016.187516
CGAACTA1951.8189894E-1216.12820624
CCTAGAT700.00259272415.8571433
TATACAG1403.473724E-815.8571435
TCTATAC1302.589477E-715.6538463
GACCGTT3200.015.6093757
GTTTAGG1557.208655E-915.51612951
Produced by FastQC (version 0.11.5)