##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1290241
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.448663466747686	31.0	31.0	34.0	30.0	34.0
2	31.558488685447138	31.0	31.0	34.0	30.0	34.0
3	31.70779025003856	31.0	31.0	34.0	30.0	34.0
4	35.415079043372515	37.0	35.0	37.0	33.0	37.0
5	35.30287287413747	37.0	35.0	37.0	33.0	37.0
6	35.38933114046136	37.0	35.0	37.0	33.0	37.0
7	35.213326812587724	37.0	35.0	37.0	32.0	37.0
8	35.273469840130645	37.0	35.0	37.0	32.0	37.0
9	36.825759683656	39.0	37.0	39.0	32.0	39.0
10	36.68893020761238	39.0	35.0	39.0	32.0	39.0
11	36.71649792558134	39.0	35.0	39.0	32.0	39.0
12	36.56489911574659	38.0	35.0	39.0	32.0	39.0
13	36.57575135187922	39.0	35.0	39.0	32.0	39.0
14	37.50055919785528	39.0	36.0	41.0	32.0	41.0
15	37.5365718497552	39.0	36.0	41.0	32.0	41.0
16	37.41093408130729	39.0	36.0	41.0	32.0	41.0
17	37.51169355182481	39.0	36.0	41.0	32.0	41.0
18	37.39377139619653	39.0	36.0	41.0	32.0	41.0
19	37.483811163960844	39.0	36.0	41.0	32.0	41.0
20	37.392987046606024	39.0	36.0	41.0	32.0	41.0
21	37.23982031263927	39.0	36.0	41.0	31.0	41.0
22	37.13726427853401	39.0	36.0	40.0	31.0	41.0
23	36.93340158931549	39.0	36.0	40.0	31.0	41.0
24	37.11349895097118	39.0	36.0	41.0	31.0	41.0
25	37.08561888825421	39.0	36.0	41.0	31.0	41.0
26	36.896703794097384	39.0	36.0	40.0	31.0	41.0
27	36.83596242872456	39.0	35.0	40.0	30.0	41.0
28	36.64409749806431	39.0	35.0	40.0	30.0	41.0
29	36.46656089831279	39.0	35.0	40.0	30.0	41.0
30	36.24818386642495	38.0	35.0	40.0	30.0	41.0
31	35.9612987031105	38.0	35.0	40.0	29.0	41.0
32	35.81590183539354	38.0	35.0	40.0	29.0	41.0
33	35.59183051848453	38.0	34.0	40.0	27.0	41.0
34	35.464595374042524	38.0	34.0	40.0	27.0	41.0
35	35.19773592685397	38.0	34.0	40.0	26.0	41.0
36	35.04028859724656	38.0	34.0	40.0	25.0	41.0
37	34.8628643796004	38.0	33.0	40.0	25.0	41.0
38	34.74511195970365	38.0	33.0	40.0	24.0	41.0
39	34.62351452170564	38.0	33.0	40.0	24.0	41.0
40	34.46488369227144	38.0	33.0	40.0	23.0	41.0
41	34.22457897400563	38.0	33.0	40.0	22.0	41.0
42	33.943626810805114	38.0	33.0	40.0	20.0	41.0
43	33.59127558339876	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	1.0
15	6.0
16	28.0
17	51.0
18	99.0
19	257.0
20	547.0
21	1123.0
22	2161.0
23	3597.0
24	5746.0
25	8786.0
26	12864.0
27	17642.0
28	24278.0
29	31119.0
30	38973.0
31	48164.0
32	59147.0
33	72215.0
34	87996.0
35	107966.0
36	137264.0
37	184174.0
38	226090.0
39	219944.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82017158034817	21.13023845932659	14.197812656705219	22.851777303620022
2	19.127976866337374	23.885460158218503	35.006173265304696	21.980389710139423
3	19.926122329084254	24.57478874101815	33.09645252321078	22.402636406686813
4	13.777581087564261	16.281531899854368	36.001336184480266	33.939550828101105
5	12.411479715805033	38.71594531564258	34.80125030905079	14.071324659501597
6	32.2966794575587	37.242809676641805	13.032991510888275	17.427519354911215
7	26.051644615230796	33.1796927860764	20.923532890366996	19.84512970832581
8	27.563687714155726	34.00426741980762	18.52925151192684	19.902793354109814
9	24.51611753153093	14.420329225315271	21.656884256507116	39.40666898664668
10	16.852355490175867	27.3882166199958	32.71946868840783	23.03995920142051
11	33.11373611596593	22.408371769305113	23.503593514699965	20.974298600028987
12	21.611311375161694	27.436114648348642	30.41160527374343	20.54096870274623
13	32.209253930079726	20.752789595122152	26.100162682785623	20.9377937920125
14	20.632346980137818	23.149396120569723	27.662583966871306	28.55567293242115
15	22.697542552127857	29.462170245713786	25.654121981862303	22.186165220296054
16	21.663007143626658	28.431122557723715	26.48590457131652	23.41996572733311
17	21.995115641186413	26.524269496938945	26.6758690818227	24.80474578005194
18	23.388266223131957	25.11569543984418	29.538667582257887	21.95737075476597
19	23.05670025987393	24.289570708108023	30.792309343758262	21.86141968825979
20	24.04651534093243	24.033649527491377	30.54723884917624	21.372596282399954
21	22.73389235034385	24.99587286406183	31.19308718293714	21.077147602657178
22	21.185809472803918	25.479503441605093	30.741156109595032	22.593530975995957
23	22.4058916124972	25.780687483966176	31.684855775006376	20.12856512853025
24	22.324588972137764	25.768209195026355	28.983189962185357	22.92401187065052
25	22.11772839337767	26.028005620655364	30.23427406197757	21.619991923989392
26	21.891104065054513	26.87474665585732	30.21001502820016	21.024134250888014
27	20.763330261555787	26.950933972800428	29.744443092414517	22.541292673229265
28	21.300594230070196	26.441416758574558	29.824893178871235	22.43309583248401
29	21.700984544747843	26.3956113625284	29.116420885710497	22.786983207013265
30	21.443048236724767	26.646494724628965	31.23059955465684	20.679857483989426
31	22.26522021854832	26.297102634314058	30.946389085449926	20.4912880616877
32	21.914433040028953	26.137830064305817	29.601059026956978	22.346677868708248
33	21.501564436411492	26.20944459213434	30.304571006501885	21.984419964952284
34	20.60188755434062	27.004722373572072	30.89422828758348	21.499161784503826
35	20.142361000774272	29.261975088374964	29.676393789997373	20.91927012085339
36	22.638328808338905	26.486989639919983	29.56184154743184	21.312840004309273
37	21.48916365237192	27.45440580480701	29.43651612373192	21.61991441908915
38	21.354692650442825	25.653269427959586	31.05528347029741	21.93675445130018
39	20.796037329460155	25.655517070066754	31.723608225130036	21.824837375343055
40	19.540922974855086	26.380575411880415	31.386849433555437	22.691652179709063
41	21.286410833324936	25.05276146084336	31.440482824526583	22.22034488130512
42	19.73298011766794	27.26188363259267	30.843927607322975	22.161208642416415
43	20.043697262759437	27.14624632142367	29.941925578244682	22.868130837572206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	142.0
2	203.0
3	580.0
4	957.0
5	957.0
6	1556.0
7	2155.0
8	2392.0
9	2629.0
10	4149.5
11	5670.0
12	5670.0
13	10941.5
14	16213.0
15	26807.0
16	37401.0
17	33839.0
18	30277.0
19	30277.0
20	31290.5
21	32304.0
22	24958.5
23	17613.0
24	19651.0
25	21689.0
26	21689.0
27	23374.5
28	25060.0
29	26982.5
30	28905.0
31	31862.0
32	34819.0
33	34819.0
34	38829.0
35	42839.0
36	47269.0
37	51699.0
38	55097.0
39	58495.0
40	58495.0
41	60618.0
42	62741.0
43	68001.5
44	73262.0
45	78937.5
46	84613.0
47	84613.0
48	114265.0
49	143917.0
50	135042.5
51	126168.0
52	115493.5
53	104819.0
54	104819.0
55	92472.5
56	80126.0
57	67808.0
58	55490.0
59	48718.0
60	41946.0
61	41946.0
62	37047.0
63	32148.0
64	27711.0
65	23274.0
66	20439.0
67	17604.0
68	17604.0
69	15191.0
70	12778.0
71	10827.0
72	8876.0
73	7332.0
74	5788.0
75	5788.0
76	4631.5
77	3475.0
78	2736.5
79	1998.0
80	1540.5
81	1083.0
82	1083.0
83	813.5
84	544.0
85	420.0
86	296.0
87	222.0
88	148.0
89	148.0
90	116.0
91	84.0
92	57.5
93	31.0
94	24.0
95	17.0
96	17.0
97	11.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1290241.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.42869836727485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.18557329161851	42.781257573234825
2	9.525241315883504	9.797415250201539
3	2.72837810158146	4.209508032543327
4	1.2386189365552067	2.5480223872036984
5	0.6716410606356935	1.727081275925482
6	0.45355926292689464	1.3995577514850464
7	0.3128253146054863	1.1261739122545391
8	0.23853565069222016	0.9814062423433462
9	0.1929462157436812	0.8930675457530026
>10	1.2483403983343238	12.24962235270765
>50	0.1131119465004369	4.057913723154365
>100	0.08001437579847989	7.44977056125656
>500	0.006970945131233466	2.5986275464712385
>1k	0.0036370148510783303	4.025873632862019
>5k	1.5154228546159708E-4	0.5024548418588631
>10k+	4.546268563847913E-4	3.6522473707445395
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18656	1.4459314190139672	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15152	1.1743542485473646	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13054	1.011748967828491	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6447	0.49967409189446005	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4504	0.3490820707139209	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4189	0.32466802713601567	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3912	0.30319916976750855	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3782	0.2931235327353572	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3547	0.2749098811772374	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3297	0.2555336561154079	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2229	0.17275842265127211	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2224	0.17237089815003553	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2026	0.15702492790106654	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1995	0.1546222759933997	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1972	0.15283966328771137	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1607	0.12455037469744025	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1582	0.1226127521912573	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1511	0.1171099042736977	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1499	0.11617984547072989	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1488	0.11532729156800939	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1467	0.11369968866281571	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1366	0.10587169373783659	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1316	0.10199644872547066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.750490024731813E-5	0.0	0.0	0.0	0.0
7	7.750490024731813E-5	0.0	0.0	0.0	0.0
8	7.750490024731813E-5	0.0	0.0	0.0	0.0
9	1.5500980049463627E-4	0.0	0.0	1.5500980049463627E-4	0.0
10	2.3251470074195442E-4	0.0	0.0	3.1001960098927254E-4	0.0
11	2.3251470074195442E-4	0.0	0.0	6.200392019785451E-4	0.0
12	2.3251470074195442E-4	0.0	0.0	8.525539027204995E-4	0.0
13	2.3251470074195442E-4	0.0	0.0	9.300588029678177E-4	0.0
14	2.3251470074195442E-4	0.0	0.0	0.0010850686034624538	0.0
15	2.3251470074195442E-4	0.0	0.0	0.0013175833042044084	0.0
16	2.3251470074195442E-4	0.0	0.0	0.0018601176059356353	0.0
17	2.3251470074195442E-4	0.0	0.0	0.003565225411376634	0.0
18	2.3251470074195442E-4	0.0	0.0	0.004495284214344452	0.0
19	2.3251470074195442E-4	0.0	0.0	0.005347838117064951	0.0
20	2.3251470074195442E-4	0.0	0.0	0.008990568428688905	0.0
21	2.3251470074195442E-4	0.0	0.0	0.015423475149216308	0.0
22	2.3251470074195442E-4	0.0	0.0	0.025111587680131078	0.0
23	2.3251470074195442E-4	0.0	0.0	0.03177700910140044	0.0
24	2.3251470074195442E-4	0.0	0.0	0.04131011183182057	0.0
25	2.3251470074195442E-4	0.0	0.0	0.047200484250616744	0.0
26	2.3251470074195442E-4	0.0	0.0	0.059213743788951057	0.0
27	2.3251470074195442E-4	0.0	0.0	0.1174974287749343	0.0
28	2.3251470074195442E-4	0.0	0.0	0.24987579839735366	0.0
29	2.3251470074195442E-4	0.0	0.0	0.42030907404120627	0.0
30	2.3251470074195442E-4	0.0	0.0	0.6615818285111076	0.0
31	3.1001960098927254E-4	0.0	0.0	0.9256410236537205	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5450	0.0	22.811008	1
GTATTGG	715	0.0	20.6993	1
CAATGCG	55	5.143565E-4	20.181818	19
ATTGGAC	835	0.0	19.053892	3
TATTGGA	780	0.0	18.73718	2
TGCGACG	60	9.237654E-4	18.5	22
TATACTG	175	0.0	17.97143	5
TAGGGGT	125	8.580173E-9	17.76	4
TTGGACC	990	0.0	17.565657	4
TGGACCC	930	0.0	17.505377	5
ACCGTTA	270	0.0	17.12963	8
CCGTTTA	270	0.0	16.444445	27
GGACCCT	980	0.0	16.42347	6
TGTTACG	160	6.2937033E-10	16.1875	16
CGAACTA	195	1.8189894E-12	16.128206	24
CCTAGAT	70	0.002592724	15.857143	3
TATACAG	140	3.473724E-8	15.857143	5
TCTATAC	130	2.589477E-7	15.653846	3
GACCGTT	320	0.0	15.609375	7
GTTTAGG	155	7.208655E-9	15.5161295	1
>>END_MODULE