FastQCFastQC Report
Wed 25 May 2016
SRR2088451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624290
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT177531.0929698514427841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154730.9526008286697573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135910.8367348195211446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65830.4052847705766827No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG24910.15335931391561852No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20280.12485455183495557No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.1225150681220718No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19620.12079123801784163No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG18890.11629696667467017No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA17260.10626181285361605No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.10115188790179093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42900.026.9522131
TATACGG1205.178663E-918.52
GTATCAA69000.016.7572482
CTCTAAT4150.016.0481931
CTAATAC4350.015.3103443
ATTGGAC5250.015.152383
TCTAATA4900.014.7244892
AATACTG5300.014.66037755
TATACAG3800.014.6052645
TACACAG2800.014.5357145
TTGGACC5750.014.4782614
GTCTAAC1152.2108445E-514.4782611
GTATTGG5050.014.2871291
ACCGTTA2353.6379788E-1214.1702128
TAGGACC3550.014.0704224
TAATACT5050.013.9207934
CTAGGGC800.00630009913.8753
GGACCGT2950.013.796616
ATACTGG4600.013.6739136
ATGCTAG3950.013.58227815
Produced by FastQC (version 0.11.5)