##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088451_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1624290
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.479538752316397	31.0	31.0	34.0	30.0	34.0
2	31.586471627603444	31.0	31.0	34.0	30.0	34.0
3	31.747494597639584	31.0	31.0	34.0	30.0	34.0
4	35.44537367095777	37.0	35.0	37.0	33.0	37.0
5	35.33043791441184	37.0	35.0	37.0	33.0	37.0
6	35.40677403665602	37.0	35.0	37.0	33.0	37.0
7	35.26701943618443	37.0	35.0	37.0	32.0	37.0
8	35.297062716633114	37.0	35.0	37.0	32.0	37.0
9	36.864515572958034	39.0	37.0	39.0	33.0	39.0
10	36.707715371023646	39.0	35.0	39.0	32.0	39.0
11	36.74711720197748	39.0	35.0	39.0	32.0	39.0
12	36.614859415498465	39.0	35.0	39.0	32.0	39.0
13	36.62141120120175	39.0	35.0	39.0	32.0	39.0
14	37.61444384931262	39.0	36.0	41.0	32.0	41.0
15	37.63005251525282	39.0	36.0	41.0	32.0	41.0
16	37.50800903779498	39.0	36.0	41.0	32.0	41.0
17	37.57243103140449	39.0	36.0	41.0	32.0	41.0
18	37.456359393950585	39.0	36.0	41.0	32.0	41.0
19	37.53625584101361	39.0	36.0	41.0	32.0	41.0
20	37.433762443898566	39.0	36.0	41.0	32.0	41.0
21	37.27746769357689	39.0	36.0	41.0	31.0	41.0
22	37.16960579699438	39.0	36.0	40.0	31.0	41.0
23	36.9806272278965	39.0	36.0	40.0	31.0	41.0
24	37.173032524980144	39.0	36.0	41.0	31.0	41.0
25	37.15041895228069	39.0	36.0	41.0	31.0	41.0
26	36.9829328506609	39.0	36.0	41.0	31.0	41.0
27	36.90601801402459	39.0	36.0	41.0	31.0	41.0
28	36.713726612858544	39.0	35.0	40.0	30.0	41.0
29	36.565804135960946	39.0	35.0	40.0	30.0	41.0
30	36.396134310991265	38.0	35.0	40.0	30.0	41.0
31	36.16731002468771	38.0	35.0	40.0	30.0	41.0
32	36.00725978735324	38.0	35.0	40.0	29.0	41.0
33	35.80410702522333	38.0	35.0	40.0	28.0	41.0
34	35.69863324898879	38.0	34.0	40.0	27.0	41.0
35	35.46158998700971	38.0	34.0	40.0	27.0	41.0
36	35.301305801303954	38.0	34.0	40.0	26.0	41.0
37	35.14985809184321	38.0	34.0	40.0	25.0	41.0
38	35.0648831181624	38.0	34.0	40.0	25.0	41.0
39	34.964957612249044	38.0	33.0	40.0	24.0	41.0
40	34.85386907510358	38.0	33.0	40.0	24.0	41.0
41	34.640118451754304	38.0	33.0	40.0	23.0	41.0
42	34.43166368074667	38.0	33.0	40.0	23.0	41.0
43	34.08287497922169	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	6.0
15	12.0
16	24.0
17	63.0
18	154.0
19	323.0
20	732.0
21	1362.0
22	2585.0
23	4355.0
24	6939.0
25	10396.0
26	14726.0
27	20956.0
28	28060.0
29	36597.0
30	46454.0
31	57799.0
32	71263.0
33	88215.0
34	108667.0
35	132859.0
36	170154.0
37	228467.0
38	293933.0
39	299186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29971248976476	21.249592129484267	13.47862758497559	22.972067795775388
2	18.668587505925665	24.00341072099194	35.10684668378184	22.221155089300556
3	19.542446238048626	26.10746849392658	32.07493735724532	22.27514791077948
4	13.726982250706463	17.115293451292565	37.19766790413042	31.960056393870552
5	12.598981708931287	39.07251783856331	34.76780624149629	13.560694211009118
6	31.095863423403458	38.29291567392522	14.360859206176238	16.250361696495084
7	26.050520535126115	32.82775858990698	22.451717365741338	18.67000350922557
8	27.096516016228627	34.76497423489648	19.346729955857636	18.79177979301726
9	25.396142314488173	14.33771063049086	21.475290742416686	38.79085631260428
10	16.67694808193118	27.76696279605243	33.28143373412383	22.274655387892555
11	33.334256813746315	23.24098529203529	23.125550240412736	20.299207653805663
12	20.658133707650727	27.646109992673722	30.92046371029804	20.77529258937751
13	30.619409095666416	21.697418564418918	26.25239335340364	21.43077898651103
14	20.85163363685056	22.80645697504756	28.172740089516036	28.169169298585846
15	23.49364953302674	29.41845360126578	25.329405463310124	21.758491402397357
16	21.734727173103323	28.50667060684976	26.764001502194805	22.994600717852105
17	21.609380098381447	27.59642674645538	27.576787396339324	23.217405758823855
18	22.469571320392294	26.125137752494936	29.35276336122244	22.05252756589033
19	23.235444409557406	25.532386458083224	30.25580407439558	20.976365057963786
20	23.190193869321366	25.588903459357624	30.221265906950112	20.999636764370894
21	22.37685388692906	26.037530243983525	30.481625818049736	21.103990051037684
22	21.50748942614927	26.533131398949696	30.238996730879336	21.720382444021695
23	21.80903656366782	26.683227748739448	30.813709374557497	20.694026313035234
24	21.874234280824236	26.866877220200823	29.271989607767086	21.98689889120785
25	21.732079862586115	26.75433574053894	30.02702719341989	21.48655720345505
26	21.665343011408062	27.19994582248244	29.990642065148464	21.144069100961037
27	21.355299854090095	27.318951665035186	29.597916628188315	21.727831852686403
28	21.267138257330895	27.137333850482364	29.87994754631254	21.715580345874198
29	21.677717648942	27.199576430317247	29.514187737411422	21.60851818332933
30	21.396979603395945	27.36537194712767	30.332452948672962	20.905195500803426
31	21.657647341299892	27.13819576553448	30.266701143268754	20.937455749896877
32	21.235370531124367	27.109383176649487	29.830879953702848	21.824366338523294
33	21.168510549224585	27.17802855401437	30.160439330415134	21.49302156634591
34	20.928713468654	27.31488835121807	30.377888184991598	21.378509995136337
35	20.61959379174901	28.394560084713937	29.767221370568066	21.21862475296899
36	21.77025038632264	27.14730743894255	29.646368567189356	21.43607360754545
37	21.08324252442606	27.20881123444705	29.752260987877783	21.955685253249115
38	20.96411355115158	26.6257257016912	30.52349026343818	21.88667048371904
39	20.678696538179757	26.580044203929106	30.784527393507314	21.956731864383823
40	20.137044493286297	27.009770422769336	30.74161633698416	22.111568746960213
41	20.752821232661656	26.059262816369	30.88998885666968	22.297927094299663
42	19.784336540888635	27.208995930529646	30.633199736500256	22.373467792081463
43	20.120114019048323	26.79127495705816	30.242013433561745	22.846597590331776
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	75.0
1	215.5
2	356.0
3	848.0
4	1340.0
5	1340.0
6	2110.5
7	2881.0
8	3325.5
9	3770.0
10	5601.5
11	7433.0
12	7433.0
13	13017.0
14	18601.0
15	28390.0
16	38179.0
17	36588.5
18	34998.0
19	34998.0
20	37336.0
21	39674.0
22	33546.5
23	27419.0
24	30223.0
25	33027.0
26	33027.0
27	36603.5
28	40180.0
29	44130.5
30	48081.0
31	52826.0
32	57571.0
33	57571.0
34	63143.5
35	68716.0
36	74205.5
37	79695.0
38	83654.0
39	87613.0
40	87613.0
41	89683.5
42	91754.0
43	96332.0
44	100910.0
45	101856.0
46	102802.0
47	102802.0
48	117272.0
49	131742.0
50	127120.0
51	122498.0
52	114982.0
53	107466.0
54	107466.0
55	98848.0
56	90230.0
57	80103.5
58	69977.0
59	63623.5
60	57270.0
61	57270.0
62	51796.0
63	46322.0
64	41014.0
65	35706.0
66	30968.0
67	26230.0
68	26230.0
69	22834.5
70	19439.0
71	16283.0
72	13127.0
73	10767.5
74	8408.0
75	8408.0
76	6709.0
77	5010.0
78	3881.0
79	2752.0
80	2091.0
81	1430.0
82	1430.0
83	1101.5
84	773.0
85	609.5
86	446.0
87	329.5
88	213.0
89	213.0
90	161.5
91	110.0
92	74.0
93	38.0
94	28.5
95	19.0
96	19.0
97	13.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1624290.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.0231807670524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.54127883032353	54.76624757598636
2	9.055058750859821	11.594673265251561
3	2.4341808082931253	4.6753199372712135
4	0.9549260910375276	2.4454962298268144
5	0.48771468922998984	1.5612522855659212
6	0.27113337707664864	1.0415292727535792
7	0.1978913100995494	0.8868741783112591
8	0.1432879180802274	0.7338998624787995
9	0.09883612800058628	0.5695022960367357
>10	0.7093585011651005	8.568582731802465
>50	0.06539608322893592	2.923793672525659
>100	0.0368825539851418	4.56304046052808
>500	0.002703305746583007	1.2582496701448334
>1k	9.654663380653596E-4	1.1107656991797337
>5k	9.654663380653596E-5	0.4069098830105835
>10k+	2.8963990141960786E-4	2.893862979326521
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17753	1.0929698514427841	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15473	0.9526008286697573	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13591	0.8367348195211446	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6583	0.4052847705766827	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2491	0.15335931391561852	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2028	0.12485455183495557	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1990	0.1225150681220718	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1962	0.12079123801784163	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1889	0.11629696667467017	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1726	0.10626181285361605	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1643	0.10115188790179093	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	6.156536086536271E-5	0.0	0.0	6.156536086536271E-5	0.0
9	6.156536086536271E-5	0.0	0.0	3.0782680432681357E-4	0.0
10	6.156536086536271E-5	6.156536086536271E-5	0.0	3.0782680432681357E-4	0.0
11	6.156536086536271E-5	6.156536086536271E-5	0.0	5.540882477882644E-4	0.0
12	6.156536086536271E-5	6.156536086536271E-5	0.0	7.387843303843525E-4	0.0
13	6.156536086536271E-5	6.156536086536271E-5	0.0	7.387843303843525E-4	0.0
14	6.156536086536271E-5	6.156536086536271E-5	0.0	8.003496912497152E-4	0.0
15	6.156536086536271E-5	6.156536086536271E-5	0.0	0.001046611134711166	0.0
16	6.156536086536271E-5	6.156536086536271E-5	0.0	0.0015391340216340677	0.0
17	6.156536086536271E-5	6.156536086536271E-5	0.0	0.002401049073749146	0.0
18	6.156536086536271E-5	6.156536086536271E-5	0.0	0.00295513732153741	0.0
19	6.156536086536271E-5	6.156536086536271E-5	0.0	0.003693921651921763	0.0
20	6.156536086536271E-5	6.156536086536271E-5	0.0	0.006649058973459173	0.0
21	6.156536086536271E-5	6.156536086536271E-5	0.0	0.012189941451341817	0.0
22	6.156536086536271E-5	6.156536086536271E-5	0.0	0.020562830529031146	0.0
23	6.156536086536271E-5	6.156536086536271E-5	0.0	0.025919016924317702	0.0
24	6.156536086536271E-5	6.156536086536271E-5	0.0	0.035092255693256746	0.0
25	6.156536086536271E-5	6.156536086536271E-5	0.0	0.0404484420885433	0.0
26	6.156536086536271E-5	6.156536086536271E-5	0.0	0.04894446188796336	0.0
27	6.156536086536271E-5	6.156536086536271E-5	0.0	0.0969038780020809	0.0
28	6.156536086536271E-5	6.156536086536271E-5	0.0	0.19362305992156573	0.0
29	6.156536086536271E-5	1.8469608259608813E-4	0.0	0.3152146476306571	0.0
30	6.156536086536271E-5	1.8469608259608813E-4	0.0	0.4675889157724298	0.0
31	6.156536086536271E-5	1.8469608259608813E-4	0.0	0.6763570544668748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4290	0.0	26.952213	1
TATACGG	120	5.178663E-9	18.5	2
GTATCAA	6900	0.0	16.757248	2
CTCTAAT	415	0.0	16.048193	1
CTAATAC	435	0.0	15.310344	3
ATTGGAC	525	0.0	15.15238	3
TCTAATA	490	0.0	14.724489	2
AATACTG	530	0.0	14.6603775	5
TATACAG	380	0.0	14.605264	5
TACACAG	280	0.0	14.535714	5
TTGGACC	575	0.0	14.478261	4
GTCTAAC	115	2.2108445E-5	14.478261	1
GTATTGG	505	0.0	14.287129	1
ACCGTTA	235	3.6379788E-12	14.170212	8
TAGGACC	355	0.0	14.070422	4
TAATACT	505	0.0	13.920793	4
CTAGGGC	80	0.006300099	13.875	3
GGACCGT	295	0.0	13.79661	6
ATACTGG	460	0.0	13.673913	6
ATGCTAG	395	0.0	13.582278	15
>>END_MODULE