FastQCFastQC Report
Wed 25 May 2016
SRR2088450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1342241
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT182871.3624229925922393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148581.1069547123057633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128700.9588442016001597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.4800926212207793No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG39960.2977110667905391No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG39470.2940604556111756No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA36140.2692512000452974No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34730.2587463801210066No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA34050.2536802258312777No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG30440.2267849067343346No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA21580.1607758964299258No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20830.15518822625743067No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.1470674789400711No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19300.14378937910554065No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17980.13395507960194927No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.1192781326155288No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14670.10929482857400422No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.1084753036153716No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC14060.10475019016704154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.023.054391
GTCATAG902.1527649E-618.51
ACGTTTA701.2193907E-418.526
TAGGTCG902.1527649E-618.521
GATCCTA500.007035482718.49999811
CGTCATT1005.8792684E-616.6526
ATTAGGT1151.2424971E-616.08695619
CGAACTA1504.671165E-916.03333324
TTATGCG1302.5897134E-715.6538464
AGTTCCG1902.7284841E-1115.57894832
GTATACG957.061087E-515.5789481
GTATTGG5700.015.5789471
CGCCAAT1201.9359904E-615.41666714
GTATCAA79000.015.3151892
GACCGTC855.3649815E-415.23529522
TACTGTG2550.015.2352947
CTAATAC2800.015.1964283
TTAACGG1101.4521498E-515.13636435
CGATTAA1353.9732913E-715.07407524
TCCGTAT750.004104882514.813
Produced by FastQC (version 0.11.5)