##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1342241
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45545993603235	31.0	31.0	34.0	30.0	34.0
2	31.571962859128874	31.0	31.0	34.0	30.0	34.0
3	31.718768835104875	31.0	31.0	34.0	30.0	34.0
4	35.420949740024334	37.0	35.0	37.0	33.0	37.0
5	35.3040221539947	37.0	35.0	37.0	33.0	37.0
6	35.38774780385937	37.0	35.0	37.0	33.0	37.0
7	35.222549452743586	37.0	35.0	37.0	32.0	37.0
8	35.275162955087794	37.0	35.0	37.0	32.0	37.0
9	36.82564233993746	39.0	37.0	39.0	32.0	39.0
10	36.68205113686737	39.0	35.0	39.0	32.0	39.0
11	36.716015976266554	39.0	35.0	39.0	32.0	39.0
12	36.57061734815134	38.0	35.0	39.0	32.0	39.0
13	36.58122125609336	39.0	35.0	39.0	32.0	39.0
14	37.52401021873121	39.0	36.0	41.0	32.0	41.0
15	37.560429907892846	39.0	36.0	41.0	32.0	41.0
16	37.43347059134686	39.0	36.0	41.0	32.0	41.0
17	37.52469191449226	39.0	36.0	41.0	32.0	41.0
18	37.401732624767085	39.0	36.0	41.0	32.0	41.0
19	37.49213665802192	39.0	36.0	41.0	32.0	41.0
20	37.397207356950055	39.0	36.0	41.0	32.0	41.0
21	37.26113268779601	39.0	36.0	41.0	31.0	41.0
22	37.15620741729689	39.0	36.0	40.0	31.0	41.0
23	36.953697584859945	39.0	36.0	40.0	31.0	41.0
24	37.141883611065374	39.0	36.0	41.0	31.0	41.0
25	37.12097082416645	39.0	36.0	41.0	31.0	41.0
26	36.93584535117017	39.0	36.0	41.0	31.0	41.0
27	36.86759605763793	39.0	35.0	40.0	31.0	41.0
28	36.66897524364104	39.0	35.0	40.0	30.0	41.0
29	36.494316594411885	39.0	35.0	40.0	30.0	41.0
30	36.31805316630918	38.0	35.0	40.0	30.0	41.0
31	36.038241269637865	38.0	35.0	40.0	29.0	41.0
32	35.90222173216285	38.0	35.0	40.0	29.0	41.0
33	35.69175431237758	38.0	34.0	40.0	28.0	41.0
34	35.5915562108444	38.0	34.0	40.0	27.0	41.0
35	35.336451501630485	38.0	34.0	40.0	26.0	41.0
36	35.17527552801621	38.0	34.0	40.0	26.0	41.0
37	35.01395502000013	38.0	34.0	40.0	25.0	41.0
38	34.902619574279136	38.0	33.0	40.0	25.0	41.0
39	34.786175507975095	38.0	33.0	40.0	24.0	41.0
40	34.644464742173724	38.0	33.0	40.0	23.0	41.0
41	34.410052293142584	38.0	33.0	40.0	23.0	41.0
42	34.151524949692345	38.0	33.0	40.0	21.0	41.0
43	33.80502905215978	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	1.0
15	7.0
16	16.0
17	54.0
18	110.0
19	260.0
20	580.0
21	1162.0
22	2148.0
23	3581.0
24	5825.0
25	8967.0
26	12876.0
27	18097.0
28	24164.0
29	31658.0
30	39582.0
31	48996.0
32	60523.0
33	75002.0
34	91015.0
35	111121.0
36	142004.0
37	189769.0
38	236568.0
39	238153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66613894226149	21.171011763163246	14.236489572289923	22.92635972228534
2	19.106255881022857	23.893324671202862	34.80433096589957	22.196088481874714
3	19.69541982401074	24.743172053304882	32.958686256789946	22.602721865894427
4	13.962097715685932	16.340657154713647	35.88565689768082	33.8115882319196
5	12.392632917635508	38.81545862479242	34.752626391236745	14.039282066335328
6	32.27460642313862	37.286672065597756	13.235402584185701	17.203318927077923
7	25.88186473219042	33.25356623735976	21.160730450045858	19.703838580403964
8	27.6171715809605	34.02764481192275	18.647843420071357	19.707340187045396
9	24.632014668006715	14.355022682215788	21.65102988211506	39.36193276766244
10	16.645147927980148	27.68385111168561	32.87248713159559	22.798513828738653
11	33.15619177182041	22.64317659794329	23.26027889179365	20.940352738442648
12	21.418582802939262	27.45952477982717	30.59100414903136	20.530888268202208
13	32.1419178821091	20.970153646029292	25.899447267666538	20.98848120419507
14	20.698369368839128	23.311312946035773	27.638553732153916	28.351763952971186
15	22.642431575253624	29.887404720910776	25.27929038078855	22.190873323047054
16	21.769041476158158	28.466944460793552	26.29416028865159	23.469853774396697
17	22.0239137382929	26.87244690037035	26.582484069552336	24.52115529178441
18	23.364656570615857	25.29866097071986	29.282893310515774	22.053789148148507
19	23.034835025900712	24.747120673560115	30.574762654396643	21.643281646142533
20	23.935641959975893	24.49835759748063	30.23413828068134	21.33186216186214
21	22.772140025524475	25.210524786532375	30.85548720386279	21.161847984080357
22	21.43244022496705	25.68055960144266	30.442968140594722	22.444032032995565
23	22.39240196060171	25.96106064410192	31.268155271668796	20.378382123627574
24	22.113018451976956	25.98557189059193	29.000827720208218	22.900581937222896
25	22.17165173765367	26.247372863740566	29.99893461755378	21.582040781051987
26	21.883402459021887	26.905898419136353	29.96645162828434	21.244247493557417
27	20.767581976709103	27.007221504930932	29.596249853789296	22.62894666457067
28	21.33335220724147	26.667789167519096	29.58283944537531	22.416019179864122
29	21.526387586133936	26.562368456931356	29.066687725974695	22.844556230960013
30	21.432067713622217	26.883175227101543	30.8503465473041	20.83441051197214
31	22.271633782606852	26.534877119682683	30.696797370963935	20.496691726746537
32	21.761367742454595	26.482651029137095	29.47279959411164	22.28318163429667
33	21.382449202490463	26.462609918785073	30.12774904059703	22.02719183812743
34	20.537742476947134	27.057883047828224	30.667890490604893	21.73648398461975
35	20.126266445444596	29.127183568375575	29.699137487232175	21.047412498947654
36	22.473013415623573	26.70585982696103	29.494554256649884	21.32657250076551
37	21.458292512298463	27.496477905234606	29.428321739538575	21.616907842928356
38	21.181814592163402	25.99384164244722	30.877837884552772	21.9465058808366
39	20.759237722584842	25.751485761498866	31.635749466750006	21.85352704916628
40	19.572118568871012	26.26182630392009	31.482423797216747	22.683631329992153
41	20.826066257847884	25.273181194733287	31.56452529761794	22.336227249800896
42	19.663309346086137	27.088428978104528	31.004789750871865	22.243471924937474
43	19.831609971681686	27.071218953973244	30.266546767681813	22.83062430666326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	159.5
2	240.0
3	598.5
4	957.0
5	957.0
6	1584.5
7	2212.0
8	2600.5
9	2989.0
10	4494.5
11	6000.0
12	6000.0
13	11490.5
14	16981.0
15	27035.5
16	37090.0
17	33831.5
18	30573.0
19	30573.0
20	31789.5
21	33006.0
22	25994.5
23	18983.0
24	21019.0
25	23055.0
26	23055.0
27	25166.0
28	27277.0
29	29815.0
30	32353.0
31	35222.0
32	38091.0
33	38091.0
34	41859.5
35	45628.0
36	49985.0
37	54342.0
38	57976.5
39	61611.0
40	61611.0
41	64058.5
42	66506.0
43	71289.0
44	76072.0
45	81862.5
46	87653.0
47	87653.0
48	116010.5
49	144368.0
50	135753.0
51	127138.0
52	116875.5
53	106613.0
54	106613.0
55	94827.5
56	83042.0
57	71766.5
58	60491.0
59	53275.0
60	46059.0
61	46059.0
62	40706.5
63	35354.0
64	30485.5
65	25617.0
66	21907.5
67	18198.0
68	18198.0
69	15381.0
70	12564.0
71	10449.5
72	8335.0
73	6939.5
74	5544.0
75	5544.0
76	4369.0
77	3194.0
78	2528.0
79	1862.0
80	1425.5
81	989.0
82	989.0
83	767.5
84	546.0
85	416.5
86	287.0
87	223.0
88	159.0
89	159.0
90	134.5
91	110.0
92	76.0
93	42.0
94	31.0
95	20.0
96	20.0
97	13.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1342241.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38611107717605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.92794012090427	46.48442214014984
2	9.401505182129197	10.41425620620108
3	2.6426412995357915	4.390968736596666
4	1.1191035838032473	2.4793118159756977
5	0.6157781925135714	1.705277968472968
6	0.39878561612635127	1.3252310658452509
7	0.2817042893085577	1.09217535409925
8	0.2182191806644228	0.9669049423560055
9	0.16034744809631343	0.7992919414083721
>10	1.072422879112158	11.321285538419753
>50	0.09716563736255888	3.734943826130395
>100	0.05520779007250453	5.573314088866402
>500	0.005129318442646858	2.019751714582024
>1k	0.0035095336712846923	3.7709607818891504
>5k	1.3498206428018048E-4	0.4817619206679954
>10k+	4.0494619284054144E-4	3.4401419583392006
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18287	1.3624229925922393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14858	1.1069547123057633	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12870	0.9588442016001597	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6444	0.4800926212207793	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3996	0.2977110667905391	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3947	0.2940604556111756	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3614	0.2692512000452974	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3473	0.2587463801210066	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3405	0.2536802258312777	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3044	0.2267849067343346	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2158	0.1607758964299258	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2083	0.15518822625743067	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1974	0.1470674789400711	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1930	0.14378937910554065	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1798	0.13395507960194927	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1601	0.1192781326155288	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1467	0.10929482857400422	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1456	0.1084753036153716	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1406	0.10475019016704154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.4900453793320275E-4	0.0	0.0	0.0	0.0
3	1.4900453793320275E-4	0.0	0.0	0.0	0.0
4	1.4900453793320275E-4	0.0	0.0	0.0	0.0
5	1.4900453793320275E-4	0.0	0.0	0.0	0.0
6	1.4900453793320275E-4	0.0	0.0	0.0	0.0
7	1.4900453793320275E-4	0.0	0.0	0.0	0.0
8	2.980090758664055E-4	0.0	0.0	0.0	0.0
9	2.980090758664055E-4	0.0	0.0	7.450226896660138E-5	0.0
10	2.980090758664055E-4	0.0	0.0	1.4900453793320275E-4	0.0
11	3.725113448330069E-4	0.0	0.0	6.705204206994125E-4	0.0
12	3.725113448330069E-4	0.0	0.0	7.450226896660138E-4	0.0
13	3.725113448330069E-4	0.0	0.0	8.195249586326152E-4	0.0
14	3.725113448330069E-4	0.0	0.0	8.940272275992165E-4	0.0
15	4.4701361379960826E-4	0.0	0.0	0.001341040841398825	0.0
16	4.4701361379960826E-4	0.0	0.0	0.001937058993131636	0.0
17	4.4701361379960826E-4	0.0	0.0	0.00268208168279765	0.0
18	4.4701361379960826E-4	0.0	0.0	0.003278099834530461	0.0
19	4.4701361379960826E-4	0.0	0.0	0.004991652020762292	0.0
20	4.4701361379960826E-4	0.0	0.0	0.007524729165626739	0.0
21	4.4701361379960826E-4	0.0	0.0	0.013410408413988249	0.0
22	4.4701361379960826E-4	0.0	0.0	0.021903667076180806	0.0
23	4.4701361379960826E-4	0.0	0.0	0.02659731002107669	0.0
24	4.4701361379960826E-4	0.0	0.0	0.03516507095223585	0.0
25	4.4701361379960826E-4	0.0	0.0	0.042466293310962785	0.0
26	4.4701361379960826E-4	0.0	0.0	0.054237651807685805	0.0
27	4.4701361379960826E-4	0.0	0.0	0.12322675287075868	0.0
28	4.4701361379960826E-4	0.0	0.0	0.25151966003124626	0.0
29	4.4701361379960826E-4	0.0	0.0	0.4209378196612978	0.0
30	4.4701361379960826E-4	0.0	0.0	0.6547259396784929	0.0
31	4.4701361379960826E-4	0.0	0.0	0.9168994241719631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5240	0.0	23.05439	1
GTCATAG	90	2.1527649E-6	18.5	1
ACGTTTA	70	1.2193907E-4	18.5	26
TAGGTCG	90	2.1527649E-6	18.5	21
GATCCTA	50	0.0070354827	18.499998	11
CGTCATT	100	5.8792684E-6	16.65	26
ATTAGGT	115	1.2424971E-6	16.086956	19
CGAACTA	150	4.671165E-9	16.033333	24
TTATGCG	130	2.5897134E-7	15.653846	4
AGTTCCG	190	2.7284841E-11	15.578948	32
GTATACG	95	7.061087E-5	15.578948	1
GTATTGG	570	0.0	15.578947	1
CGCCAAT	120	1.9359904E-6	15.416667	14
GTATCAA	7900	0.0	15.315189	2
GACCGTC	85	5.3649815E-4	15.235295	22
TACTGTG	255	0.0	15.235294	7
CTAATAC	280	0.0	15.196428	3
TTAACGG	110	1.4521498E-5	15.136364	35
CGATTAA	135	3.9732913E-7	15.074075	24
TCCGTAT	75	0.0041048825	14.8	13
>>END_MODULE