FastQCFastQC Report
Wed 25 May 2016
SRR2088449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658619
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85141.292704887043951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70871.0760394097346113No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60330.9160075855691986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.4561058821564516No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG18560.28180177006736823No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA17970.27284363190251115No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG17700.2687441449457122No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA16340.24809487731146537No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15010.227901108227974No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG14540.2207649642661387No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA10130.15380667730508837No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9520.14454487343972766No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.14059721933318048No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC8480.12875425701353893No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8370.12708409566076898No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.11934062029792641No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA6860.10415733527274493No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6720.10203167536921953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAACG250.0054946629.64
GGTATGA551.9010597E-523.5454541
GTATACT502.7004044E-422.24
GGTATCA23100.021.6233771
CGTTATA450.003824098720.55555710
TGTTACG909.4629286E-820.55555716
TACCCTC1101.7516868E-920.1818185
TGGATAG555.139889E-420.1818185
GTGTAGA656.896412E-519.9230771
GTATTAG759.255924E-619.7333341
TAGTACT851.2436558E-619.5882364
GTATTGG2650.019.547171
ACCGTTA1650.019.0606068
TTAGGTC500.0070317718.520
GACCGTT1700.018.57
TTAGACC500.0070317718.54
ATACTAC609.2311075E-418.56
TGGACCC3550.018.2394375
CCGTTAT1850.018.09
CGAACTA953.601659E-617.52631629
Produced by FastQC (version 0.11.5)