##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658619
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41916798634719	31.0	31.0	34.0	30.0	34.0
2	31.537483734905916	31.0	31.0	34.0	30.0	34.0
3	31.688281085119016	31.0	31.0	34.0	30.0	34.0
4	35.4034988362012	37.0	35.0	37.0	33.0	37.0
5	35.2789898256807	37.0	35.0	37.0	33.0	37.0
6	35.364234861126086	37.0	35.0	37.0	33.0	37.0
7	35.195077882660534	37.0	35.0	37.0	32.0	37.0
8	35.24601476726301	37.0	35.0	37.0	32.0	37.0
9	36.79789832968682	39.0	37.0	39.0	32.0	39.0
10	36.64457144418852	39.0	35.0	39.0	32.0	39.0
11	36.681604994693444	39.0	35.0	39.0	32.0	39.0
12	36.52604768462495	38.0	35.0	39.0	32.0	39.0
13	36.53957143659688	38.0	35.0	39.0	32.0	39.0
14	37.47049356304631	39.0	36.0	41.0	32.0	41.0
15	37.494903730381296	39.0	36.0	41.0	32.0	41.0
16	37.36755696388959	39.0	36.0	41.0	32.0	41.0
17	37.45306467016591	39.0	36.0	41.0	32.0	41.0
18	37.328475188234776	39.0	36.0	40.0	32.0	41.0
19	37.410754928114734	39.0	36.0	41.0	32.0	41.0
20	37.33159687163595	39.0	36.0	41.0	31.0	41.0
21	37.17319876893925	39.0	36.0	40.0	31.0	41.0
22	37.06903384202399	39.0	36.0	40.0	31.0	41.0
23	36.86437378818406	39.0	36.0	40.0	30.0	41.0
24	37.05525045587813	39.0	36.0	40.0	31.0	41.0
25	37.03239353860123	39.0	36.0	41.0	31.0	41.0
26	36.81681518449969	39.0	36.0	40.0	30.0	41.0
27	36.758335243896695	39.0	35.0	40.0	30.0	41.0
28	36.55180005435616	39.0	35.0	40.0	30.0	41.0
29	36.37766599505936	38.0	35.0	40.0	30.0	41.0
30	36.18592691677586	38.0	35.0	40.0	30.0	41.0
31	35.90869531550107	38.0	35.0	40.0	29.0	41.0
32	35.7613506443027	38.0	34.0	40.0	28.0	41.0
33	35.53576498704107	38.0	34.0	40.0	27.0	41.0
34	35.405730779099905	38.0	34.0	40.0	27.0	41.0
35	35.1456714731886	38.0	34.0	40.0	26.0	41.0
36	34.98656127442421	38.0	33.0	40.0	25.0	41.0
37	34.8127840223255	38.0	33.0	40.0	25.0	41.0
38	34.711701302270356	38.0	33.0	40.0	24.0	41.0
39	34.5871573701943	38.0	33.0	40.0	24.0	41.0
40	34.4485370145714	38.0	33.0	40.0	23.0	41.0
41	34.21322342659413	38.0	33.0	40.0	22.0	41.0
42	33.95496789494381	38.0	33.0	40.0	21.0	41.0
43	33.599038290726504	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	3.0
16	12.0
17	31.0
18	63.0
19	143.0
20	330.0
21	632.0
22	1151.0
23	1963.0
24	2999.0
25	4608.0
26	6742.0
27	9271.0
28	12473.0
29	16131.0
30	20167.0
31	24519.0
32	30525.0
33	37545.0
34	45872.0
35	55484.0
36	70011.0
37	92869.0
38	115049.0
39	110023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61556225981941	21.19783972220662	14.020700890803333	23.16589712717064
2	19.290515457343318	23.731778160059154	34.66040305548428	22.317303327113247
3	19.947799866083425	24.552434715670213	32.729241033131444	22.770524385114914
4	13.967103894664442	16.476900909326943	35.84530661884944	33.710688577159175
5	12.688215796993404	38.550664344636274	34.66131405258579	14.099805805784527
6	32.584999825392224	37.15425762087033	13.283704235681023	16.97703831805642
7	26.216826420130605	33.04687535585824	21.030064422678365	19.70623380133279
8	27.557662320704384	34.16724995786639	18.48823067661273	19.786857044816504
9	24.90438326255392	14.295366516908867	21.3387406072403	39.46150961329691
10	16.715126651372035	27.48053123277646	32.798628645696525	23.005713470154976
11	33.41765117617317	22.66636704984217	23.058854967743112	20.857126806241546
12	21.569222873922556	27.2623474269646	30.440512648435593	20.72791705067725
13	32.14954321087002	20.901310165664825	25.625437468399788	21.32370915506537
14	20.81400627677003	23.214179973550717	27.33644185788749	28.635371891791767
15	22.98840452522627	29.48700234885419	25.24312235146572	22.28147077445382
16	22.03808271550016	28.286156336212592	26.262072609505648	23.4136883387816
17	22.148920696183986	26.652131201802558	26.563764482956003	24.63518361905745
18	23.40761502477153	25.322379099297166	29.020268167180113	22.24973770875119
19	23.208106659540643	24.77061852148207	30.31676887548036	21.704505943496923
20	24.120166591003297	24.46179050406988	29.870228462889774	21.54781444203705
21	23.015734438271597	25.23431604615111	30.34759094408148	21.40235857149581
22	21.680516353157138	25.77787765005261	30.040129422321556	22.501476574468697
23	22.575571005391584	25.95779957760101	30.81599528710833	20.650634129899075
24	22.52561799765874	26.007297086783105	28.459397618349914	23.00768729720825
25	22.453649226639378	26.167177078098263	29.551834975911717	21.827338719350642
26	22.17442861502629	26.77374931485426	29.648856167222625	21.40296590289682
27	21.1547191927351	26.87486999312197	29.295996623237407	22.674414190905516
28	21.71407141306279	26.50758632836283	29.31467206381838	22.463670194756
29	21.973098255592383	26.49483236894168	28.638560381647054	22.893508993818884
30	21.734113349296027	26.812466691668472	30.563041758588806	20.89037820044669
31	22.501780240169204	26.457633320629988	30.18194130445675	20.858645134744062
32	21.938328532884718	26.396900180529258	29.170886354629914	22.493884931956106
33	21.70010279083962	26.38019856700156	29.658877135339246	22.26082150681957
34	20.926514418806626	26.828560973795167	30.396025623311807	21.8488989840864
35	20.52537202844133	28.745754373924832	29.424447214550447	21.304426383083392
36	22.66712621409343	26.5760629438264	29.179085328543515	21.57772551353666
37	21.685526837215445	27.33583452648648	29.114860032886998	21.863778603411077
38	21.576814516435146	25.786835788217466	30.575188386608946	22.061161308738438
39	20.992713541516416	25.708034538936776	31.263294863950176	22.035957055596636
40	19.865050962696188	26.28803602689871	30.948089866827406	22.898823143577697
41	21.11858297437517	25.02068722584681	31.275745157670826	22.584984642107198
42	19.80583615109798	26.955341403755433	30.7423563547362	22.49646609041039
43	20.190276927935574	26.844351590221354	29.88176775950891	23.083603722334157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	23.0
1	66.0
2	109.0
3	288.0
4	467.0
5	467.0
6	732.5
7	998.0
8	1149.5
9	1301.0
10	2025.5
11	2750.0
12	2750.0
13	5293.0
14	7836.0
15	12715.5
16	17595.0
17	16065.5
18	14536.0
19	14536.0
20	15060.0
21	15584.0
22	12247.0
23	8910.0
24	9924.5
25	10939.0
26	10939.0
27	11890.5
28	12842.0
29	13988.0
30	15134.0
31	16594.5
32	18055.0
33	18055.0
34	20279.5
35	22504.0
36	24868.0
37	27232.0
38	29100.5
39	30969.0
40	30969.0
41	31847.5
42	32726.0
43	35219.0
44	37712.0
45	40300.5
46	42889.0
47	42889.0
48	56171.5
49	69454.0
50	65470.0
51	61486.0
52	57078.0
53	52670.0
54	52670.0
55	46589.0
56	40508.0
57	35097.5
58	29687.0
59	26336.5
60	22986.0
61	22986.0
62	20328.5
63	17671.0
64	15301.5
65	12932.0
66	11323.0
67	9714.0
68	9714.0
69	8446.0
70	7178.0
71	6109.5
72	5041.0
73	4189.0
74	3337.0
75	3337.0
76	2759.5
77	2182.0
78	1693.5
79	1205.0
80	947.5
81	690.0
82	690.0
83	521.0
84	352.0
85	279.0
86	206.0
87	164.0
88	122.0
89	122.0
90	89.0
91	56.0
92	36.0
93	16.0
94	13.5
95	11.0
96	11.0
97	7.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	658619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.20733912034159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.46615828178086	52.05897314104312
2	7.748945325116999	9.330867580286096
3	2.175962697634118	3.9302677214901194
4	0.9889723490160233	2.3817357439139415
5	0.5692182329378095	1.713555759198414
6	0.3783730175630425	1.3668499549443036
7	0.2566964382117671	1.081850665447977
8	0.19511665900034314	0.9397963885169367
9	0.14809840076198988	0.8024949574071643
>10	0.9402337255858777	10.68912190715627
>50	0.08004420152627442	3.3312286037719248
>100	0.04508815249486955	5.1948709860425675
>500	0.004306171867479237	1.739503228333942
>1k	0.002026433819990229	2.139531017911351
>5k	7.599126824963359E-4	3.299352344535902
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8514	1.292704887043951	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7087	1.0760394097346113	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6033	0.9160075855691986	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.4561058821564516	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1856	0.28180177006736823	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1797	0.27284363190251115	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1770	0.2687441449457122	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1634	0.24809487731146537	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1501	0.227901108227974	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1454	0.2207649642661387	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1013	0.15380667730508837	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	952	0.14454487343972766	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.14059721933318048	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	848	0.12875425701353893	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	837	0.12708409566076898	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	786	0.11934062029792641	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	686	0.10415733527274493	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.10203167536921953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.0366570050362955E-4	0.0
10	0.0	0.0	0.0	3.0366570050362955E-4	0.0
11	0.0	0.0	0.0	4.554985507554443E-4	0.0
12	0.0	0.0	0.0	0.0010628299517627035	0.0
13	0.0	0.0	0.0	0.0012146628020145182	1.5183285025181477E-4
14	0.0	3.0366570050362955E-4	0.0	0.001366495652266333	1.5183285025181477E-4
15	0.0	3.0366570050362955E-4	0.0	0.0018219942030217773	1.5183285025181477E-4
16	0.0	3.0366570050362955E-4	0.0	0.002732991304532666	1.5183285025181477E-4
17	0.0	3.0366570050362955E-4	0.0	0.00349215555579174	1.5183285025181477E-4
18	0.0	3.0366570050362955E-4	0.0	0.004099486956798999	1.5183285025181477E-4
19	1.5183285025181477E-4	3.0366570050362955E-4	0.0	0.004554985507554444	1.5183285025181477E-4
20	1.5183285025181477E-4	3.0366570050362955E-4	0.0	0.006832478261331665	1.5183285025181477E-4
21	1.5183285025181477E-4	3.0366570050362955E-4	0.0	0.013209457971907887	1.5183285025181477E-4
22	1.5183285025181477E-4	3.0366570050362955E-4	0.0	0.020497434783994996	1.5183285025181477E-4
23	1.5183285025181477E-4	3.0366570050362955E-4	0.0	0.024900587441297625	1.5183285025181477E-4
24	1.5183285025181477E-4	4.554985507554443E-4	0.0	0.03234039710363655	1.5183285025181477E-4
25	1.5183285025181477E-4	4.554985507554443E-4	0.0	0.03735088116194644	1.5183285025181477E-4
26	1.5183285025181477E-4	4.554985507554443E-4	0.0	0.04706818357806258	1.5183285025181477E-4
27	1.5183285025181477E-4	6.073314010072591E-4	0.0	0.10931965218130664	1.5183285025181477E-4
28	1.5183285025181477E-4	6.073314010072591E-4	0.0	0.22835660677872943	1.5183285025181477E-4
29	1.5183285025181477E-4	6.073314010072591E-4	0.0	0.40858220002763357	1.5183285025181477E-4
30	1.5183285025181477E-4	6.073314010072591E-4	0.0	0.6387608010093848	1.5183285025181477E-4
31	1.5183285025181477E-4	6.073314010072591E-4	0.0	0.8979394763892327	1.5183285025181477E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAACG	25	0.00549466	29.6	4
GGTATGA	55	1.9010597E-5	23.545454	1
GTATACT	50	2.7004044E-4	22.2	4
GGTATCA	2310	0.0	21.623377	1
CGTTATA	45	0.0038240987	20.555557	10
TGTTACG	90	9.4629286E-8	20.555557	16
TACCCTC	110	1.7516868E-9	20.181818	5
TGGATAG	55	5.139889E-4	20.181818	5
GTGTAGA	65	6.896412E-5	19.923077	1
GTATTAG	75	9.255924E-6	19.733334	1
TAGTACT	85	1.2436558E-6	19.588236	4
GTATTGG	265	0.0	19.54717	1
ACCGTTA	165	0.0	19.060606	8
TTAGGTC	50	0.00703177	18.5	20
GACCGTT	170	0.0	18.5	7
TTAGACC	50	0.00703177	18.5	4
ATACTAC	60	9.2311075E-4	18.5	6
TGGACCC	355	0.0	18.239437	5
CCGTTAT	185	0.0	18.0	9
CGAACTA	95	3.601659E-6	17.526316	29
>>END_MODULE